scholarly journals Role of the Cys 2-Cys 10 disulfide bond for the structure, stability, and folding kinetics of ribonuclease T1

1994 ◽  
Vol 3 (2) ◽  
pp. 227-239 ◽  
Author(s):  
Lorenz M. Mayr ◽  
Dieter Willbold ◽  
Olfert Landt ◽  
Franz X. Schmidm
Keyword(s):  
Disulfide Bond ◽  
Structure Stability ◽  
Folding Kinetics ◽  
Ribonuclease T1 ◽  
Kinetics Of

scholarly journals Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance in Saccharomyces cerevisiae

2020 ◽  
Vol 295 (27) ◽  
pp. 8958-8971 ◽  
Author(s):  
Katarina Jurikova ◽  
Martin Gajarsky ◽  
Mona Hajikazemi ◽  
Jozef Nosek ◽  
Katarina Prochazkova ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Secondary Structures ◽  
Telomere Maintenance ◽  
Folding Kinetics ◽  
Mobility Shift ◽  
Dna Structures ◽  
Electrophoretic Mobility Shift Assays ◽  
Kinetics Of

The ends of eukaryotic chromosomes typically contain a 3′ ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 in Saccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs of S. cerevisiae form distinct Hoogsteen pairing–based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13 in vitro as demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance.

Stability and Folding Kinetics of Ribonuclease T1 are Strongly Altered by the Replacement of Cis-proline 39 with Alanine

1993 ◽  
Vol 231 (3) ◽  
pp. 897-912 ◽  
Author(s):  
Lorenz M. Mayr ◽  
Olfert Landt ◽  
Ulrich Hahn ◽  
Franz X. Schmid
Keyword(s):  
Folding Kinetics ◽  
Ribonuclease T1 ◽  
Kinetics Of

scholarly journals The Role of Electrostatic Relaxation on the Folding Kinetics of a Bacterial Ribozyme

2011 ◽  
Vol 100 (3) ◽  
pp. 236a
Author(s):  
Joon Ho Roh ◽  
Robert M. Briber ◽  
Duncan Kilburn ◽  
Reza Behrouzi ◽  
Liang Guo ◽  
...  
Keyword(s):  
Folding Kinetics ◽  
Kinetics Of

Role of Entropy in Protein Thermostability:  Folding Kinetics of a Hyperthermophilic Cold Shock Protein at High Temperatures Using19F NMR†

Biochemistry ◽  
2002 ◽  
Vol 41 (39) ◽  
pp. 11670-11680 ◽  
Author(s):  
Benjamin Schuler ◽  
Werner Kremer ◽  
Hans Robert Kalbitzer ◽  
Rainer Jaenicke
Keyword(s):  
High Temperatures ◽  
Cold Shock ◽  
Cold Shock Protein ◽  
Folding Kinetics ◽  
Protein Thermostability ◽  
Kinetics Of
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