scholarly journals Modulating long-range energetics via helix stabilization: A case study using T4 lysozyme

2018 ◽  
Vol 27 (12) ◽  
pp. 2084-2093
Author(s):  
Sabriya N. Rosemond ◽  
Kambiz M. Hamadani ◽  
Jamie H. D. Cate ◽  
Susan Marqusee
Keyword(s):  
Long Range ◽  
T4 Lysozyme

scholarly journals Modulating long-range energetics via helix stabilization: a case study using T4 lysozyme

2018 ◽  
Author(s):  
Sabriya N. Rosemond ◽  
Kambiz M. Hamadani ◽  
Jamie H.D. Cate ◽  
Susan Marqusee
Keyword(s):  
Protein Folding ◽  
Long Range ◽  
Salt Bridge ◽  
Globular Protein ◽  
Full Length ◽  
T4 Lysozyme ◽  
Long Distance ◽  
Terminal Fragment ◽  
The Stability

Cooperative protein folding requires distant regions of a protein to interact and provide mutual stabilization. The mechanism of this long-distance coupling remains poorly understood. Here, we use T4 lysozyme (T4L*) as a model to investigate long-range communications across a globular protein. T4L* is composed of two structurally distinct subdomains, although it behaves in a two-state manner at equilibrium. The subdomains of T4L* are connected via two topological connections: the N-terminal helix that is structurally part of the C-terminal subdomain (the A-helix) and a long helix that spans both subdomains (the C-helix). To understand the role that the C-helix plays in cooperative folding, we analyzed a circularly permuted version of T4L* (CP13*), whose subdomains are connected only by the C-helix. We demonstrate that when isolated as individual fragments, both subdomains of CP13* can fold autonomously into marginally stable conformations. The energetics of the N-terminal subdomain depend on the formation of a salt bridge known to be important for stability in the full-length protein. We show that the energetic contribution of the salt bridge to the stability of the N-terminal fragment increases when the C-helix is stabilized, such as occurs upon folding of the C-terminal subdomain. These results suggest a model where long-range energetic coupling is mediated by helix stabilization.

scholarly journals The Long-Range Memory and the Fractal Dimension: a Case Study for Alcântara

2017 ◽  
Vol 9 (4) ◽  
pp. 461-468 ◽  
Author(s):  
Cleber Souza Corrêa ◽  
Daniel Andrade Schuch ◽  
Antonio Paulo De Queiroz ◽  
Gilberto Fernando Fisch ◽  
Felipe Do Nascimento Corrêa ◽  
...  
Keyword(s):  
Fractal Dimension ◽  
Long Range

Centralizing Integrated Long Range Planning and Budgeting

2011 ◽  
pp. 55-75
Author(s):  
Taylor Valore
Keyword(s):  
Long Range ◽  
State Of The Art ◽  
Technical Aspects ◽  
American University ◽  
Independent Events ◽  
Long Range Planning ◽  
American University In Cairo ◽  
Annual Planning

Upon relocation to a new, state-of-the-art, 260-acre campus outside of Cairo, Egypt, the American University in Cairo (AUC) sought to revamp its annual planning and budgeting processes to address several deficiencies. Primarily, long-range planning and annual budgeting were two independent events with little synchronization. This case study will detail the process and technical aspects of AUC’s transition to a centralized and synchronized planning and budgeting cycle focused on determining appropriate workflows and leveraging database technologies to track planning initiatives throughout an approvals process. Readers will be able to weigh the drawbacks of centralization against the benefits of standardized budget review and planning.

scholarly journals Validation of a Long-Range Trajectory Model Using Gas Balloon Tracks from the Gordon Bennett Cup 95

1997 ◽  
Vol 36 (6) ◽  
pp. 711-720 ◽  
Author(s):  
Kathrin Baumann ◽  
Andreas Stohl
Keyword(s):  
Long Range ◽  
Computation Time ◽  
Wind Fields ◽  
Time Step ◽  
Long Distance ◽  
Trajectory Model ◽  
Trajectory Calculations ◽  
Stochastic Errors ◽  
Divergent Wind

Abstract In September 1995, 18 gas balloon teams competed at the Gordon Bennett Cup, a long-distance ballooning event. The landing positions, travel times of all teams, and detailed information on the tracks of four teams are available. A special version of the trajectory model FLEXTRA (flexible trajectories) is used that allows the heights of calculated trajectories to be adjusted to the respective balloon heights at every computation time step. The comparison of calculated and observed balloon trajectories allows a validation of the trajectory model. In this case study, the agreement between calculated and balloon trajectories was good, with average relative transport errors of less than 20% of the travel distance after 46 h of travel time. Most of the trajectory errors originate from interpolation errors and from amplifications of small position disturbances in divergent wind fields. Trajectory ensembles, taking into account stochastic errors occurring during the trajectory calculations, are shown to be very reliable in assessing the uncertainties of the computed trajectories. In the present study, the balloon tracks were enveloped by the ensemble trajectories most of the time, suggesting that errors in the analyzed wind fields were relatively small.

Long-Range Transport of Air Pollution: A Case Study, August 1970

1973 ◽  
Vol 101 (5) ◽  
pp. 404-411 ◽  
Author(s):  
FRANKLIN P. HALL ◽  
CLAUDE E. DUCHON ◽  
LAURENCE G. LEE ◽  
RICHARD R. HAGAN
Keyword(s):  
Air Pollution ◽  
Long Range ◽  
Long Range Transport ◽  
Range Transport
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