scholarly journals Recombinant expression, purification, and biophysical characterization of the transmembrane and membrane proximal domains of HIV-1 gp41

2014 ◽  
Vol 23 (11) ◽  
pp. 1607-1618 ◽  
Author(s):  
Zhen Gong ◽  
Sarah A. Kessans ◽  
Lusheng Song ◽  
Katerina Dörner ◽  
Ho-Hsien Lee ◽  
...  
Keyword(s):  
Recombinant Expression ◽  
Biophysical Characterization ◽  
Hiv 1

Biophysical characterization of V3-lipopeptide liposomes influencing HIV-1 infectivity

2007 ◽  
Vol 355 (4) ◽  
pp. 963-969 ◽  
Author(s):  
Apostolos K. Rizos ◽  
Stavroula Baritaki ◽  
Ioannis Tsikalas ◽  
David C. Doetschman ◽  
Demetrios A. Spandidos ◽  
...  
Keyword(s):  
Biophysical Characterization ◽  
Hiv 1

scholarly journals Biophysical characterization of the SARS-CoV-2 E protein

2021 ◽  
Author(s):  
Aujan Mehregan ◽  
Sergio Perez-Conesa ◽  
Yuxuan Zhuang ◽  
Ahmad Elbahnsi ◽  
Diletta Pasini ◽  
...  
Keyword(s):  
Recombinant Expression ◽  
Structural Data ◽  
Md Simulations ◽  
Full Length ◽  
Coarse Grained ◽  
Biophysical Characterization ◽  
Viral Budding ◽  
Nmr Structures ◽  
E Protein

SARS-CoV-2 is the virus responsible for the COVID-19 pandemic which continues to wreak havoc across the world, over a year and a half after its effects were first reported in the general media. Current fundamental research efforts largely focus on the SARS-CoV-2 Spike protein. Since successful antiviral therapies are likely to target multiple viral components, there is considerable interest in understanding the biophysical role of its other proteins, in particular structural membrane proteins. Here, we have focused our efforts on the characterization of the full-length E protein from SARS-CoV-2, combining experimental and computational approaches. Recombinant expression of the full-length E protein from SARS-CoV-2 reveals that this membrane protein is capable of independent multimerization, possibly as a tetrameric or smaller species. Fluorescence microscopy shows that the protein localizes intracellularly, and coarse-grained MD simulations indicate it causes bending of the surrounding lipid bilayer, corroborating a potential role for the E protein in viral budding. Although we did not find robust electrophysiological evidence of ion-channel activity, cells transfected with the E protein exhibited reduced intracellular Ca2+, which may further promote viral replication. However, our atomistic MD simulations revealed that previous NMR structures are relatively unstable, and result in models incapable of ion conduction. Our study highlights the importance of using high-resolution structural data obtained from a full-length protein to gain detailed molecular insights, and eventually permitting virtual drug screening.

scholarly journals De Novo Design and Biophysical Characterization of an Affinity-Enhanced Protein Displaying the Structure of the Broadly Neutralizing HIV-1 2F5 Antibody Epitope

2016 ◽  
Vol 110 (3) ◽  
pp. 346a
Author(s):  
Isabelle Freire Tabosa Viana ◽  
Eduardo Nascimento ◽  
Jodi Craigo ◽  
Marco Krieger ◽  
Robbie Mailliard ◽  
...  
Keyword(s):  
De Novo ◽  
De Novo Design ◽  
Biophysical Characterization ◽  
Antibody Epitope ◽  
Hiv 1

Biophysical characterization of recombinant HIV-1 subtype C virus infectivity factor

Amino Acids ◽  
2010 ◽  
Vol 40 (3) ◽  
pp. 981-989 ◽  
Author(s):  
Daniela Gallerano ◽  
Siva Charan Devanaboyina ◽  
Ines Swoboda ◽  
Birgit Linhart ◽  
Irene Mittermann ◽  
...  
Keyword(s):  
Virus Infectivity ◽  
Subtype C ◽  
Biophysical Characterization ◽  
Hiv 1

scholarly journals Biophysical characterization of small molecule antiviral-loaded nanolipogels for HIV-1 chemoprophylaxis and topical mucosal application

2016 ◽  
Vol 36 ◽  
pp. 122-131 ◽  
Author(s):  
R. Ramanathan ◽  
Y. Jiang ◽  
B. Read ◽  
S. Golan-Paz ◽  
K.A. Woodrow
Keyword(s):  
Small Molecule ◽  
Biophysical Characterization ◽  
Hiv 1

scholarly journals Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0136507 ◽  
Author(s):  
Zhen Gong ◽  
Jose M. Martin-Garcia ◽  
Sasha M. Daskalova ◽  
Felicia M. Craciunescu ◽  
Lusheng Song ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Binding Protein ◽  
Maltose Binding Protein ◽  
Protein Fusion ◽  
Biophysical Characterization ◽  
Maltose Binding Protein Fusion ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document