Quantitative proteomics analysis reveals novel insights into mechanisms of action of disulfiram (DSF)

2021 ◽  
pp. 2100031
Author(s):  
Peng Zheng ◽  
Chenglinzi Liu ◽  
Yaoqin Wu ◽  
Ruifeng Xu ◽  
Ying Chen ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Mechanisms Of Action ◽  
Proteomics Analysis

Quantitative Proteomics Analysis Reveals Unique But Overlapping Protein Signatures in HIV Infection

2019 ◽  
Author(s):  
Maha Al-Mozaini ◽  
Ibtihag S. Alsharif ◽  
Al-Hussain J. Alzahrani ◽  
Zakia Shinwari ◽  
Magid Halim ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Quantitative Proteomics ◽  
Proteomics Analysis ◽  
Protein Signatures

scholarly journals Quantitative Proteomics Analysis Reveals Unique but Overlapping Protein Signatures in HIV Infections

2021 ◽  
Author(s):  
Maha Al-Mozaini ◽  
Ibtihag Alsharif ◽  
Alhusain Alzahrani ◽  
Zakia Shinwari ◽  
Magid Halim ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Hiv Infections ◽  
Proteomics Analysis ◽  
Protein Signatures

A Gel-Free Quantitative Proteomics Analysis of Factors Released From Hypoxic-Conditioned Placentae

2009 ◽  
Vol 17 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Richard T. Blankley ◽  
Nicola J. Robinson ◽  
John D. Aplin ◽  
Ian P. Crocker ◽  
Simon J. Gaskell ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Proteomics Analysis

scholarly journals Quantitative proteomics analysis reveals similar release profiles following specific PAR-1 or PAR-4 stimulation of platelets

2014 ◽  
Vol 103 (1) ◽  
pp. 140-146 ◽  
Author(s):  
Thijs C. van Holten ◽  
Onno B. Bleijerveld ◽  
Patrick Wijten ◽  
Philip G. de Groot ◽  
Albert J.R. Heck ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Proteomics Analysis ◽  
Stimulation Of ◽  
Release Profiles

scholarly journals Comparative Proteomics Analysis of Mouse Habu Nephritis Models with and without Unilateral Nephrectomy

2016 ◽  
Vol 39 (5) ◽  
pp. 1761-1776 ◽  
Author(s):  
Lei Chen ◽  
Yang Lu ◽  
Jun Wen ◽  
Xu Wang ◽  
Lingling Wu ◽  
...  
Keyword(s):  
Renal Injury ◽  
Quantitative Proteomics ◽  
Mesangial Cells ◽  
Unilateral Nephrectomy ◽  
Label Free ◽  
Proteomics Analysis ◽  
Single Kidney ◽  
Regulation Of Actin Cytoskeleton ◽  
Insight Into

Background/Aims: Individuals possessing a single kidney are at greater risk of renal injury upon exposure to harmful stimuli. This study aimed to explore the pathogenesis of renal injury in glomerulonephritis with versus without unilateral nephrectomy (UNX). Methods: Histological analysis and label-free quantitative proteomics were performed on two models—the Habu snake venom-induced glomerulonephritis model with versus without UNX (HabuU and Habu models, respectively). The role of villin 1, a differentially expressed protein (DEP) in mouse mesangial cells, was investigated. Results: Persistent mesangiolysis and focal hypercellularity together with reduced activation of cell proliferation in the HabuU model induced more serious renal injury compared with that in the Habu model. The DEPs between the two models were identified by label-free liquid chromatography-mass spectrometry. The KEGG pathway results indicated that regulation of actin cytoskeleton and focal adhesion were specifically enriched in the HabuU model. The cytoskeleton regulation protein villin 1 was downregulated in the HabuU model, but unchanged in the Habu model. Knockdown of villin 1 promoted apoptosis and inhibited the proliferation of mouse mesangial cells, suggesting villin 1 to be involved in qlomerular lesion self-repair insufficiency. Conclusion: By assessing the proteomic profiles of the two models, this study identified several important differences, particularly villin 1 expression, in regulatory mechanisms between the two models. Our findings provide novel insight into the mechanism of serious renal injury in glomerulonephritis with UNX.

scholarly journals iTRAQ-Based Quantitative Proteomics Analysis of the Protective Effect of Yinchenwuling Powder on Hyperlipidemic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-12
Author(s):  
Zheyu Zhang ◽  
Wenbo Wang ◽  
Ling Jin ◽  
Xin Cao ◽  
Gonghui Jian ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Serum Levels ◽  
Treated Group ◽  
Research Approach ◽  
Therapeutic Effects ◽  
Proteomics Analysis ◽  
Chinese Medicine Formula ◽  
Calcitonin Gene ◽  
Proteomics Technology ◽  
Proteomics Research

Yinchenwuling powder (YCL) is an effective traditional Chinese medicine formula to modulate lipid levels. In this study, we established hyperlipidemic rat models and treated them with YCL. The serum concentrations of lipid, malondialdehyde (MDA), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP) were measured. Adventitia-free vascular proteins between hyperlipidemic rats and YCL-treated rats were identified using iTRAQ-based quantitative proteomics research approach. Proteins with 1.3-fold difference were analyzed through bioinformatics, and proteomic results were verified by Western blot. The results showed that the serum levels of TC, TG, LDL-C, ET-1, and MDA were significantly decreased, whereas the HDL-C and CGRP levels were significantly increased in the YCL-treated group. Proteomics technology identified 4,382 proteins, and 15 proteins were selected on the basis of their expression levels and bioinformatics. Of these proteins, 2 (Adipoq and Gsta1) were upregulated and 13 (C3, C4, C6, Cfh, Cfp, C8g, C8b, Lgals1, Fndc1, Fgb, Fgg, Kng1, and ApoH) were downregulated in the YCL-treated rats. Their functions were related to immunity, inflammation, coagulation and hemostasis, oxidation and antioxidation, and lipid metabolism and transport. The validated results of ApoH were consistent with the proteomics results. This study enhanced our understanding on the therapeutic effects and mechanism of YCL on hyperlipidemia.

scholarly journals Quantitative proteomics analysis reveals the tolerance of wheat to salt stress in response to Enterobacter cloacae SBP-8

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0183513 ◽  
Author(s):  
Rajnish Prakash Singh ◽  
Ashish Runthala ◽  
Shahid Khan ◽  
Prabhat Nath Jha
Keyword(s):  
Salt Stress ◽  
Quantitative Proteomics ◽  
Enterobacter Cloacae ◽  
Proteomics Analysis
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