Measuring fucosylated alpha‐fetoprotein in hepatocellular carcinoma: A comparison of μTAS and parallel reaction monitoring

2021 ◽  
pp. 2000096
Author(s):  
Kwang Hoe Kim ◽  
Sang Yoon Lee ◽  
Je‐Hyun Baek ◽  
Soo‐Youn Lee ◽  
Jin Young Kim ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Alpha Fetoprotein ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
Parallel Reaction Monitoring

scholarly journals PRM-MS Quantitative Analysis of Isomeric N-Glycopeptides Derived from Human Serum Haptoglobin of Patients with Cirrhosis and Hepatocellular Carcinoma

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 563
Author(s):  
Cristian D. Gutierrez Reyes ◽  
Yifan Huang ◽  
Mojgan Atashi ◽  
Jie Zhang ◽  
Jianhui Zhu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Operating Characteristic ◽  
Roc Curves ◽  
Glycosylation Site ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
Serum Haptoglobin ◽  
Parallel Reaction Monitoring ◽  
Early Hcc ◽  
Auc Value

Currently, surveillance strategies have inadequate performance for cirrhosis and early detection of hepatocellular carcinoma (HCC). The glycosylation of serum haptoglobin has shown to have significant differences between cirrhosis and HCC, thus can be used for diagnosis. We performed a comprehensive liquid chromatography—parallel reaction monitoring—mass spectrometry (LC-PRM-MS) approach, where a targeted parallel reaction monitoring (PRM) strategy was coupled to a powerful LC system, to study the site-specific isomerism of haptoglobin (Hp) extracted from cirrhosis and HCC patients. We found that our strategy was able to identify a large number of isomeric N-glycopeptides, mainly located in the Hp glycosylation site Asn207. Four N-glycopeptides were found to have significant changes in abundance between cirrhosis and HCC samples (p < 0.05). Strategic combinations of the significant N-glycopeptides, either with alpha-fetoprotein (AFP) or themselves, better estimate the areas under the curve (AUC) of their respective receiver operating characteristic (ROC) curves with respect to AFP. The combination of AFP with the isomeric sialylated fucosylated N-glycopeptides Asn207 + 5-6-1-2 and Asn207 + 5-6-1-3, resulted with an AUC value of 0.98, while the AUC value for AFP alone was 0.85. When comparing cirrhosis vs. early HCC, the isomeric N-glycopeptide Asn207 + 5-6-0-1 better estimated AUC with respect to AFP (AUCAFP = 0.81, and AUCAsn207 + 5-6-0-1 = 0.88, respectively).

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