BoxCar increases the depth and reproducibility of diabetic urinary proteome analysis

2021 ◽  
pp. 2000092
Author(s):  
Shu Ye ◽  
Linhui Zhai ◽  
Hao Hu ◽  
Minjia Tan ◽  
Shichun Du
Keyword(s):  
Proteome Analysis ◽  
Urinary Proteome

scholarly journals Urinary proteome analysis enables assessment of renoprotective treatment in type 2 diabetic patients with microalbuminuria

2010 ◽  
Vol 11 (1) ◽  
Author(s):  
Sten Andersen ◽  
Harald Mischak ◽  
Petra Zürbig ◽  
Hans-Henrik Parving ◽  
Peter Rossing
Keyword(s):  
Proteome Analysis ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Urinary Proteome ◽  
Type 2 Diabetic

Advances in urinary proteome analysis and applications in systems biology

Bioanalysis ◽  
2014 ◽  
Vol 6 (19) ◽  
pp. 2549-2569 ◽  
Author(s):  
Szymon Filip ◽  
Jerome Zoidakis ◽  
Antonia Vlahou ◽  
Harald Mischak
Keyword(s):  
Systems Biology ◽  
Proteome Analysis ◽  
Urinary Proteome

Physiologic changes of urinary proteome by caffeine and excessive water intake

2017 ◽  
Vol 55 (7) ◽  
Author(s):  
Paleerath Peerapen ◽  
Nardtaya Ausakunpipat ◽  
Suchitra Sutthimethakorn ◽  
Siripat Aluksanasuwan ◽  
Arada Vinaiphat ◽  
...  
Keyword(s):  
Western Blotting ◽  
Proteome Analysis ◽  
Caffeine Intake ◽  
Water Drinking ◽  
Urinary Proteome ◽  
Flight Mass Spectrometry ◽  
Proteomics Approach ◽  
Prostaglandin D Synthase ◽  
Post Hoc ◽  
Excessive Water

AbstractBackground:Diurnal variations and physiologic changes of urinary proteome have been suggested in the urinary proteomics field. However, no clear evidence has been demonstrated. The present study thus aimed to define changes in urinary proteome by physiological stimuli, i.e. caffeine intake and excessive water drinking, both of which cause physiologic diuresis.Methods:Urine samples were collected from 30 healthy individuals under three different conditions: (i) morning void as the control; (ii) after drinking a cup of coffee; and (iii) after drinking 1 L of water within 20 min. Thereafter, differentially excreted proteins were analyzed by 2-DE proteomics approach and validated by Western blotting and ELISA.Results:Spot matching, quantitative intensity analysis, and ANOVA followed by Tukey’s post-hoc multiple comparisons and the Bonferroni correction revealed significant differences in levels of five protein spots among three different conditions. These proteins were identified by quadrupole time-of-flight mass spectrometry (Q-TOF MS) and/or MS/MS analyses as kininogen 1 isoform 3, β-actin, prostaglandin D synthase (PGDS), fibrinogen α-chain and immunoglobulin light chain. Among these, the decreased level of immunoglobulin was successfully validated by Western blotting and ELISA.Conclusions:These data indicated that caffeine intake and excessive water drinking could affect urinary excretion of some proteins and may affect urinary proteome analysis.

scholarly journals An individual urinary proteome analysis in normal human beings to define the minimal sample number to represent the normal urinary proteome

2012 ◽  
Vol 10 (1) ◽  
pp. 70 ◽  
Author(s):  
Xuejiao Liu ◽  
Chen Shao ◽  
Lilong Wei ◽  
Jindan Duan ◽  
Shuzhen Wu ◽  
...  
Keyword(s):  
Proteome Analysis ◽  
Human Beings ◽  
Sample Number ◽  
Urinary Proteome ◽  
Minimal Sample ◽  
Normal Human

Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease

2010 ◽  
Vol 25 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Cécile Caubet ◽  
Chrystelle Lacroix ◽  
Stéphane Decramer ◽  
Jens Drube ◽  
Jochen H. H. Ehrich ◽  
...  
Keyword(s):  
Renal Disease ◽  
Biomarker Discovery ◽  
Proteome Analysis ◽  
Urinary Proteome

Pretransplant urinary proteome analysis does not predict development of chronic kidney disease after liver transplantation

2015 ◽  
Vol 35 (7) ◽  
pp. 1893-1901 ◽  
Author(s):  
David Milongo ◽  
Jean-Loup Bascands ◽  
Antoine Huart ◽  
Laure Esposito ◽  
Benjamin Breuil ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Liver Transplantation ◽  
Kidney Disease ◽  
Proteome Analysis ◽  
Urinary Proteome
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