scholarly journals A Systematic Review of the Root Canal Microbiota Associated with Apical Periodontitis: Lessons from Next‐Generation Sequencing

2020 ◽  
Vol 14 (3) ◽  
pp. 1900060 ◽  
Author(s):  
Daniel Manoil ◽  
Khaled Al‐Manei ◽  
Georgios N. Belibasakis
Keyword(s):  
Systematic Review ◽  
Next Generation Sequencing ◽  
Root Canal ◽  
Apical Periodontitis ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Metagenomic next generation sequencing for the diagnosis of tuberculosis meningitis: A systematic review and meta-analysis

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243161
Author(s):  
Guocan Yu ◽  
Wuchen Zhao ◽  
Yanqin Shen ◽  
Pengfei Zhu ◽  
Hong Zheng
Keyword(s):  
Systematic Review ◽  
Next Generation Sequencing ◽  
Early Diagnosis ◽  
Meta Analysis ◽  
Characteristic Curve ◽  
Extrapulmonary Tuberculosis ◽  
Cochrane Library ◽  
Next Generation ◽  
Summary Receiver Operating Characteristic ◽  
Generation Sequencing

Background Tuberculous meningitis (TBM) is a severe form of extrapulmonary tuberculosis and its early diagnosis is very difficult leading to present with severe disability or die. The current study aimed to assess the accuracy of metagenomic next generation sequencing (mNGS) for TBM, and to identify a new test for the early diagnosis of TBM. Methods We searched for articles published in Embase, PubMed, Cochrane Library, China National Knowledge Infrastructure, and Wanfang Data up to June 30, 2020 for studies that assessed the efficacy of mNGS for the diagnosis of TBM. Then, the accuracy between mNGS and a composite reference standard (CRS) in these articles was compared using the meta-analysis approach. Results Four independent studies with 342 samples comparing mNGS and a CRS were included in this study. The sensitivity of mNGS for TBM diagnosis ranged from 27% to 84%. The combined sensitivity of mNGS was 61%, and the I2 value was 92%. Moreover, the specificity of mNGS for TBM diagnosis ranged from 96% to 100%. The combined specificity of mNGS was 98%, and the I2 value was 74%. The heterogeneity between studies in terms of sensitivity and specificity was significant. The area under the curve (AUC) of the summary receiver operating characteristic curve (SROC) of mNGS for TBM was 0.98. Conclusions The sensitivity of mNGS for TBM diagnosis was moderate. Furthermore, the specificity was extremely high, and the AUC of the SROC indicated a very good diagnostic efficacy. mNGS could be used as an early diagnostic method for TBM, however, the results should be treated with caution for the heterogeneity between studies was extremely significant. Systematic review registration INPLASY202070100.

scholarly journals The apical root canal system microbial communities determined by next-generation sequencing

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Luciana Carla Neves de Brito ◽  
Janet Doolittle-Hall ◽  
Chun-Teh Lee ◽  
Kevin Moss ◽  
Wilson Bambirra Júnior ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Microbial Communities ◽  
Root Canal ◽  
Next Generation ◽  
Canal System ◽  
Root Canal System ◽  
Generation Sequencing

scholarly journals Improving hearing loss gene testing: a systematic review of gene evidence toward more efficient next-generation sequencing–based diagnostic testing and interpretation

2015 ◽  
Vol 18 (6) ◽  
pp. 545-553 ◽  
Author(s):  
Ahmad N. Abou Tayoun ◽  
Saeed H. Al Turki ◽  
Andrea M. Oza ◽  
Mark J. Bowser ◽  
Amy L. Hernandez ◽  
...  
Keyword(s):  
Systematic Review ◽  
Hearing Loss ◽  
Next Generation Sequencing ◽  
Diagnostic Testing ◽  
Next Generation ◽  
Gene Testing ◽  
Generation Sequencing
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