scholarly journals Quantification of Urinary Protein Biomarkers of Autosomal Dominant Polycystic Kidney Disease by Parallel Reaction Monitoring

2018 ◽  
Vol 12 (5) ◽  
pp. 1700157 ◽  
Author(s):  
Navin Rauniyar ◽  
Xiaoqing Yu ◽  
Jennifer Cantley ◽  
Edward Z. Voss ◽  
Justin Belcher ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Polycystic Kidney Disease ◽  
Autosomal Dominant ◽  
Urinary Protein ◽  
Reaction Monitoring ◽  
Parallel Reaction ◽  
Polycystic Kidney ◽  
Protein Biomarkers ◽  
Parallel Reaction Monitoring ◽  
Autosomal Dominant Polycystic Kidney

scholarly journals Coregulation Analysis of Mechanistic Biomarkers in Autosomal Dominant Polycystic Kidney Disease

2021 ◽  
Vol 22 (13) ◽  
pp. 6885
Author(s):  
Johannes Leierer ◽  
Paul Perco ◽  
Benedikt Hofer ◽  
Susanne Eder ◽  
Alexander Dzien ◽  
...  
Keyword(s):  
Growth Factor ◽  
Kidney Disease ◽  
Polycystic Kidney Disease ◽  
Autosomal Dominant ◽  
Healthy Controls ◽  
Molecular Processes ◽  
Polycystic Kidney ◽  
Protein Biomarkers ◽  
Autosomal Dominant Polycystic Kidney ◽  
End Stage

Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary kidney disorder leading to deterioration of kidney function and end stage kidney disease (ESKD). A number of molecular processes are dysregulated in ADPKD but the exact mechanism of disease progression is not fully understood. We measured protein biomarkers being linked to ADPKD-associated molecular processes via ELISA in urine and serum in a cohort of ADPKD patients as well as age, gender and eGFR matched CKD patients and healthy controls. ANOVA and t-tests were used to determine differences between cohorts. Spearman correlation coefficient analysis was performed to assess coregulation patterns of individual biomarkers and renal function. Urinary epidermal growth factor (EGF) and serum apelin (APLN) levels were significantly downregulated in ADPKD patients. Serum vascular endothelial growth factor alpha (VEGFA) and urinary angiotensinogen (AGT) were significantly upregulated in ADPKD patients as compared with healthy controls. Arginine vasopressin (AVP) was significantly upregulated in ADPKD patients as compared with CKD patients. Serum VEGFA and VIM concentrations were positively correlated and urinary EGF levels were negatively correlated with urinary AGT levels. Urinary EGF and AGT levels were furthermore significantly associated with estimated glomerular filtration rate (eGFR) in ADPKD patients. In summary, altered protein concentrations in body fluids of ADPKD patients were found for the mechanistic markers EGF, APLN, VEGFA, AGT, AVP, and VIM. In particular, the connection between EGF and AGT during progression of ADPKD warrants further investigation.

Overt proteinuria and microalbuminuria in autosomal dominant polycystic kidney disease.

1994 ◽  
Vol 5 (6) ◽  
pp. 1349-1354
Author(s):  
A B Chapman ◽  
A M Johnson ◽  
P A Gabow ◽  
R W Schrier
Keyword(s):  
Mean Arterial Pressure ◽  
Kidney Disease ◽  
Arterial Pressure ◽  
Polycystic Kidney Disease ◽  
Autosomal Dominant ◽  
Urinary Protein Excretion ◽  
Urinary Protein ◽  
Polycystic Kidney ◽  
Protein Excretion ◽  
Autosomal Dominant Polycystic Kidney

The amount of proteinuria is a prognostic indicator in a variety of glomerular disorders. To examine the importance of urinary protein excretion in autosomal dominant polycystic kidney disease, this study determined the clinical characteristics of autosomal dominant polycystic kidney disease patients with established proteinuria and the frequency of microalbuminuria in hypertensive autosomal dominant polycystic kidney disease patients without proteinuria. In 270 autosomal dominant polycystic kidney disease patients, mean 24-h urinary protein excretion was 259 +/- 22 mg/day. Forty-eight of 270 autosomal dominant poly-cystic kidney disease patients had over proteinuria (> 300 mg/day). The patients with established proteinuria had higher mean arterial pressures, larger renal volumes, and lower creatinine clearances than did their nonproteinuric counterparts (all P < 0.0001), a greater pack year smoking history (P < 0.05), and the projection of a more aggressive course of renal disease (P < 0.05). All autosomal dominant polycystic kidney disease patients with established proteinuria were hypertensive, as compared with 67% without established proteinuria (P < 0.001). Forty-nine patients with hypertension and left ventricular hypertrophy without established proteinuria were examined for microalbuminuria; 41% demonstrated microalbuminuria. Those with microalbuminuria had higher mean arterial pressure, larger renal volumes and increased filtration fraction. Therefore, established proteinuria and microalbuminuria in autosomal dominant polycystic kidney disease patients are associated with increased mean arterial pressure and more severe renal cystic involvement.

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