Proteomic analysis of the kidney filtration barrier-Problems and perspectives

2015 ◽  
Vol 9 (11-12) ◽  
pp. 1053-1068 ◽  
Author(s):  
Markus M. Rinschen ◽  
Thomas Benzing ◽  
Kavee Limbutara ◽  
Trairak Pisitkun
Keyword(s):  
Proteomic Analysis ◽  
Filtration Barrier ◽  
Barrier Problems

Effect of Heme Oxygenase-1 Deficiency on Glomerular Proteomics

2016 ◽  
Vol 43 (6) ◽  
pp. 441-450 ◽  
Author(s):  
Maria G. Detsika ◽  
Vasiliki Lygirou ◽  
Vassileios Frantzis ◽  
Jerome Zoidakis ◽  
Vassilios Atsaves ◽  
...  
Keyword(s):  
Glomerular Filtration ◽  
Heme Oxygenase ◽  
Proteomic Analysis ◽  
Bioinformatic Analysis ◽  
Fibrin Clot ◽  
Heme Oxygenase 1 ◽  
Glomerular Injury ◽  
Filtration Barrier ◽  
Glomerular Lesions

Background: The cytoprotective effect of heme oxygenase (HO)-1 in various forms of renal glomerular injury is established. However, little is known on the role of HO-1 in preserving glomerular structural/functional integrity in the absence of injury. The present study addressed this question in HO-1-deficient rats. Methods: HO-1-deficient rats were generated using zinc finger nuclease-mediated HO-1 gene (Hmox1) disruption and studied. Glomeruli were isolated from HO-1-deficient (Hmox1-/-) rats and their wild type (WT) littermates for proteomic analysis. Results: Glomerular lesions were characterized and differentially expressed proteins important for preserving integrity of the glomerular filtration barrier were identified. HO-1-deficient (Hmox1-/-) rats developed albuminuria with decreased glomerular filtration rate. In albuminuric rats, there were lesions resembling focal and segmental glomerulosclerosis (FSGS). Western blot analysis of the integral slit diaphragm proteins, nephrin and podocin revealed a significant decrease in nephrin, with no change in podocin. Proteomic analysis of glomerular protein lysates from Hmox1-/- and WT rats revealed differential expression of proteins previously linked with FSGS pathogenesis. Specifically, α-actinin-4, actin related protein 3, cytokeratins and novel candidates including transgelin-2 and lamins. Bioinformatic analysis predicted the upregulation of pathways implicated in platelet aggregation and fibrin clot formation. Conclusion: HO-1 is a putative regulator of proteins important in preserving glomerular structural stability and integrity, and in minimizing the activity of proinflammatory pathways.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Proteomic analysis of the anti-obesity effect of Taeumjowui-Tang in high fat diet-induced obese mice

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
E Changkyun Park ◽  
SY Lee ◽  
SH Yun ◽  
WY Kim ◽  
Y Yi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat

Proteomic analysis of human testicular interstitial fluid reflects disordered spermatogenesis in Klinefelter's syndrome

2014 ◽  
Author(s):  
Robert I McLachlan ◽  
Andrew N Stephens ◽  
Adam Rainczuk ◽  
Caroline Foo ◽  
Mark R Condina ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Interstitial Fluid ◽  
Klinefelter’S Syndrome ◽  
Klinefelter's Syndrome

Proteomic analysis of nuclear receptor coregulator complexes: II

2013 ◽  
Author(s):  
SY Jung ◽  
A Malovannaya ◽  
J Wei ◽  
BW O'Malley ◽  
J Qin
Keyword(s):  
Nuclear Receptor ◽  
Proteomic Analysis
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