Differential characterization of biogenic amine-producing bacteria involved in food poisoning using MALDI-TOF mass fingerprinting

2010 ◽  
Vol 4 (8-9) ◽  
pp. 777-777
Author(s):  
Inmaculada C. Fernández-No ◽  
Karola Böhme ◽  
José M. Gallardo ◽  
Jorge Barros-Velázquez ◽  
Benito Cañas ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Food Poisoning ◽  
Maldi Tof

Differential characterization of biogenic amine-producing bacteria involved in food poisoning using MALDI-TOF mass fingerprinting

2010 ◽  
pp. NA-NA ◽  
Author(s):  
Inmaculada C. Fernández-No ◽  
Karola Böhme ◽  
José M. Gallardo ◽  
Jorge Barros-Velázquez ◽  
Benito Cañas ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Food Poisoning ◽  
Maldi Tof

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

Characterization of polyethers using different methods: SFC, LAC and SEC with different detectors, and MALDI-TOF-MS

1996 ◽  
Vol 110 (1) ◽  
pp. 231-240 ◽  
Author(s):  
Bernd Trathnigg ◽  
Bernhard Maier ◽  
Günther Schulz ◽  
Ralph-Peter Krüger ◽  
Ulrich Just
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product

2021 ◽  
Vol 12 (1) ◽  
pp. 85-93
Author(s):  
Wallapat Phongtang ◽  
Ekachai Chukeatirote
Keyword(s):  
Bacillus Cereus ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Morphological Analysis ◽  
Food Poisoning ◽  
Fermented Soybean ◽  
Foodborne Pathogenic Bacteria ◽  
Ph Range ◽  
Potential Use

Abstract Bacillus cereus is considered to be an important food poisoning agent causing diarrhea and vomiting. In this study, the occurrence of B. cereus bacteriophages in Thai fermented soybean products (Thua Nao) was studied using five B. cereus sensu lato indicator strains (four B. cereus strains and one B. thuringiensis strain). In a total of 26 Thua Nao samples, there were only two bacteriophages namely BaceFT01 and BaceCM02 exhibiting lytic activity against B. cereus. Morphological analysis revealed that these two bacteriophages belonged to the Myoviridae. Both phages were specific to B. cereus and not able to lyse other tested bacteria including B. licheniformis and B. subtilis. The two phages were able to survive in a pH range between 5 and 12. However, both phages were inactive either by treatment of 50°C for 2 h or exposure of UV for 2 h. It should be noted that both phages were chloroform-insensitive, however. This is the first report describing the presence of bacteriophages in Thua Nao products. The characterization of these two phages is expected to be useful in the food industry for an alternative strategy including the potential use of the phages as a biocontrol candidate against foodborne pathogenic bacteria.

Characterization of New Amphiphilic Block Copolymers of N-Vinylpyrrolidone and Vinyl Acetate, 2 - Chromatographic Separation and Analysis by MALDI-TOF and FT-IR Coupling

2010 ◽  
Vol 211 (15) ◽  
pp. 1678-1688 ◽  
Author(s):  
Nick Fandrich ◽  
Jana Falkenhagen ◽  
Steffen M. Weidner ◽  
Bastiaan Staal ◽  
Andreas F. Thünemann ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Vinyl Acetate ◽  
Chromatographic Separation ◽  
Amphiphilic Block Copolymers ◽  
Maldi Tof ◽  
Ft Ir

Multidimensional Characterization of α,ω-Telechelic Poly(ε-caprolactone)s via Online Coupling of 2D Chromatographic Methods (LC/SEC) and ESI-TOF/MALDI-TOF-MS

2012 ◽  
Vol 45 (24) ◽  
pp. 9779-9790 ◽  
Author(s):  
Haitham Barqawi ◽  
Elena Ostas ◽  
Bo Liu ◽  
Jean-François Carpentier ◽  
Wolfgang H. Binder
Keyword(s):  
Maldi Tof Ms ◽  
Chromatographic Methods ◽  
Maldi Tof ◽  
Tof Ms

Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry

2011 ◽  
Vol 54 (2) ◽  
pp. 273-278 ◽  
Author(s):  
Jan Matysiak ◽  
Christian E.H. Schmelzer ◽  
Reinhard H.H. Neubert ◽  
Zenon J. Kokot
Keyword(s):  
Mass Spectrometry ◽  
Honeybee Venom ◽  
Maldi Tof

Isolation and characterization of biogenic amine-producing bacteria in fermented soybean pastes

2010 ◽  
Vol 48 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Jin Seok Moon ◽  
Seung Kee Cho ◽  
Hwa Young Choi ◽  
Ji Eun Kim ◽  
So-Young Kim ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Fermented Soybean ◽  
Isolation And Characterization
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