Oxidative stress occurs early in Down syndrome pregnancy: A redox proteomics analysis of amniotic fluid

2011 ◽  
Vol 5 (3-4) ◽  
pp. 167-178 ◽  
Author(s):  
Marzia Perluigi ◽  
Fabio di Domenico ◽  
Ada Fiorini ◽  
Annalisa Cocciolo ◽  
Alessandra Giorgi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Down Syndrome ◽  
Amniotic Fluid ◽  
Proteomics Analysis ◽  
Redox Proteomics

scholarly journals Oxidative Stress in HPV-Driven Viral Carcinogenesis: Redox Proteomics Analysis of HPV-16 Dysplastic and Neoplastic Tissues

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e34366 ◽  
Author(s):  
Federico De Marco ◽  
Elona Bucaj ◽  
Cesira Foppoli ◽  
Ada Fiorini ◽  
Carla Blarzino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Proteomics Analysis ◽  
Hpv 16 ◽  
Redox Proteomics ◽  
Viral Carcinogenesis ◽  
Neoplastic Tissues

Early oxidative stress in amniotic fluid of pregnancies with Down syndrome

2007 ◽  
Vol 40 (3-4) ◽  
pp. 177-180 ◽  
Author(s):  
S. Perrone ◽  
M. Longini ◽  
C.V. Bellieni ◽  
G. Centini ◽  
A. Kenanidis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Down Syndrome ◽  
Amniotic Fluid

scholarly journals Redox proteomics analysis of HNE-modified proteins in Down syndrome brain: clues for understanding the development of Alzheimer disease

2014 ◽  
Vol 71 ◽  
pp. 270-280 ◽  
Author(s):  
Fabio Di Domenico ◽  
Gilda Pupo ◽  
Antonella Tramutola ◽  
Alessandra Giorgi ◽  
Maria Eugenia Schininà ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Alzheimer Disease ◽  
Proteomics Analysis ◽  
Redox Proteomics ◽  
Down Syndrome Brain ◽  
Modified Proteins

scholarly journals Ozone effect on inflammatory and proteomic profile of human macrophages and airway epithelial cells

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
L Falcone ◽  
E Aruffo ◽  
P Di Carlo ◽  
P Del Boccio ◽  
M C Cufaro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Air Pollution ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Urban Areas ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Proteomics Analysis ◽  
Human Macrophages ◽  
Tnf Α

Abstract Background Reactive oxygen species (ROS) and oxidative stress in the respiratory system are involved in lung inflammation and tumorigenesis. Ozone (O3) is one of the main components of air pollution in urban areas able to act as strong pro-oxidant agent, however its effects on human health is still poorly investigated. In this study the effect of O3 has been evaluated in THP-1 monocytes differentiated into macrophages with PMA and in HBEpC (primary human bronchial epithelial) cells, two model systems for in vitro studies and translational research. Methods Cell viability, ROS and pro-inflammatory cytokines like interleukin-8(IL-8) and tumor necrosis factor(TNF-α) have been tested in the above-mentioned cell lines not exposed to any kind of pollution (basal condition-b.c.) or exposed to O3 at a concentration of 120 ppb. In HBEpC a labelfree shotgun proteomics analysis has been also performed in the same conditions. Results Ozone significantly increased the production of IL-8 and TNF-α in THP-1 whereas no changes were shown in HBEpC. In both cell lines lipopolysaccharide(LPS) caused an increase of IL-8 and TNF-α production in b.c. and O3 treatment potentiated this effect. Ozone exposure increased ROS formation in a time dependent manner in both cell lines and in THP-1 cells a decrease in catalase activity was also shown. Finally, according to these data, functional proteomics analysis revealed that in HBEpC exposure to O3 many differential proteins are related to oxidative stress and inflammation. Conclusions Our results indicate that O3, at levels that can be reached in urban areas, causes an increase of pro-inflammatory agents either per se or potentiating the effect of immune response stimulators in cell models of human macrophages and human airway epithelial cells. Interestingly, the proteomic analysis showed that besides the dysregulated proteins, O3 induced the expression of AKR1D1 and AKR1B10, proteins recognized to play a significant role in cancer development. Key messages This study adds new pieces of information on the association between O3 exposure and detrimental effects on respiratory system. This study suggests the need for further research on the mechanisms involved and for a continued monitoring/re-evaluation of air pollution standards aimed at safeguarding human health.

Maternal serum and amniotic fluid levels of macrophage inhibitory cytokine 1 in Down syndrome and chromosomally normal pregnancies

2004 ◽  
Vol 24 (3) ◽  
pp. 224-226 ◽  
Author(s):  
Euan M. Wallace ◽  
Budi Marjono ◽  
David A. Brown ◽  
Jennifer Crossley ◽  
Stephen Tong ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Amniotic Fluid ◽  
Maternal Serum ◽  
Fluid Levels

Bacterial vaginosis and biomarkers of oxidative stress in amniotic fluid

2012 ◽  
Vol 25 (7) ◽  
pp. 1050-1054 ◽  
Author(s):  
Mirjana Bogavac ◽  
Neda Lakic ◽  
Natasa Simin ◽  
Aleksandra Nikolic ◽  
Jan Sudji ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amniotic Fluid ◽  
Bacterial Vaginosis ◽  
Biomarkers Of Oxidative Stress
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