Comparative proteomic analysis of differentially expressed proteins in an in vitro cellular carcinogenesis model of oral squamous cell carcinoma

2009 ◽  
Vol 3 (3) ◽  
pp. 322-337 ◽  
Author(s):  
Lai-ping Zhong ◽  
Lei Zhang ◽  
Xiao Yang ◽  
Hong-ya Pan ◽  
Xiao-jian Zhou ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Analysis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Comparative Proteomic Analysis

Comparative proteomic analysis of oral squamous cell carcinoma and adjacent non-tumour tissue from Thailand

2013 ◽  
Vol 58 (11) ◽  
pp. 1677-1685 ◽  
Author(s):  
Pitak Chanthammachat ◽  
Waraporn Promwikorn ◽  
Kowit Pruegsanusak ◽  
Sittiruk Roytrakul ◽  
Chantragan Srisomsap ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Analysis ◽  
Tumour Tissue ◽  
Comparative Proteomic Analysis

Analysis of differentially expressed proteins in oral squamous cell carcinoma by MALDI-TOF MS

2010 ◽  
Vol 40 (5) ◽  
pp. 369-379 ◽  
Author(s):  
Uta J. E. Thiel ◽  
Ralph Feltens ◽  
Boris Adryan ◽  
Rita Gieringer ◽  
Christoph Brochhausen ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Identification of differentially expressed, tumor-associated proteins in oral squamous cell carcinoma by proteomic analysis

2006 ◽  
Vol 27 (7) ◽  
pp. 1417-1423 ◽  
Author(s):  
Dritan Turhani ◽  
Kurt Krapfenbauer ◽  
Dietmar Thurnher ◽  
Hanno Langen ◽  
Michael Fountoulakis
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Proteomic Analysis ◽  
Differentially Expressed ◽  
Associated Proteins

scholarly journals Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11548
Author(s):  
Yin-Ling Wong ◽  
Anand Ramanathan ◽  
Kar Mun Yuen ◽  
Wan Mahadzir Wan Mustafa ◽  
Mannil Thomas Abraham ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Acute Phase ◽  
Squamous Cell ◽  
Bioinformatics Analysis ◽  
Differentially Expressed ◽  
Complement C3 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Differentially Expressed Proteins

Background Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to identify the differentially expressed serum proteins as potential biomarkers for oral squamous cell carcinoma (OSCC). Methods Comparative proteomics profiling of serum samples from OSCC patients, oral potentially malignant disorder (OPMD) patients, and healthy individuals were performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) (n = 60) and bioinformatics analysis. The enzyme-linked immunosorbent assay (ELISA) (n = 120) and immunohistochemistry (IHC) (n = 70) were used to confirm our findings. Results The 2-DE analysis revealed that 20 differentially expressed proteins were detected in OPMD and OSCC (p < 0.05). Bioinformatics analysis indicated that the activation of classical complement, liver X receptor/retinoid X receptor (LXR/RXR) activation, and acute phase response signaling pathway are associated with the development and progression of OSCC. Most of the detected proteins are acute-phase proteins and were related to inflammation and immune responses, including apolipoprotein A-I (APOA1), complement C3 (C3), clusterin (CLU), and haptoglobin (HP). The expression levels of CLU and HP in ELISA are consistent with the findings from the 2-DE analysis, except for the mean serum level of HP in OPMD, whereby it was slightly higher than that in control. IHC results demonstrated that CLU and HP are significantly decreased in OSCC tissues. Conclusion Decreased expression of CLU and HP could serve as complementary biomarkers of OSCC. These proteins may assist in predicting the outcomes of OSCC patients. However, a larger cohort is needed for further investigation.

Cyclosporine A Suppresses the Malignant Progression of Oral Squamous Cell Carcinoma in vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 248-255 ◽  
Author(s):  
Ling Gao ◽  
Jianwei Dong ◽  
Nanyang Zhang ◽  
Zhanxian Le ◽  
Wenhao Ren ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cyclosporine A ◽  
Migration And Invasion ◽  
Signal Molecules ◽  
Cox 2

Background:The Oral Squamous Cell Carcinoma (OSCC) is one of the most frequent cancer types. Failure of treatment of OSCC is potentially lethal because of local recurrence, regional lymph node metastasis, and distant metastasis. Chemotherapy plays a vital role through suppression of tumorigenesis. Cyclosporine A (CsA), an immunosuppressant drug, has been efficiently used in allograft organ transplant recipients to prevent rejection, and also has been used in a subset of patients with autoimmunity related disorders. The present study aims to investigate novel and effective chemotherapeutic drugs to overcome drug-resistance in the treatment of OSCC.Methods:Cells were incubated in the standard way. Cell viability was assayed using the MTT assay. Cell proliferation was determined using colony formation assay. The cell cycle assay was performed using flow cytometry. Apoptosis was assessed using fluorescence-activated cell sorting after stained by the Annexin V-fluorescein isothiocyanate (FITC). Cell migration and invasion were analyzed using wound healing assay and tranwell. The effect of COX-2, c-Myc, MMP-9, MMP-2, and NFATc1 protein expression was determined using Western blot analysis while NFATc1 mRNA expression was determined by RT-PCR.Results:In vitro studies indicated that CsA inhibited partial OSCC growth by inducing cell cycle arrest, apoptosis, and the migration and invasion of OSCC cells. We also demonstrated that CsA could inhibit the expression of NFATc1 and its downstream genes COX-2, c-Myc, MMP-9, and MMP-2 in OSCC cells. Furthermore, we analyzed the expression of NFATc1 in head and neck cancer through the Oncomine database. The data was consistent with the experimental findings.Conclusion:The present study initially demonstrated that CsA could inhibit the progression of OSCC cells and can mediate the signal molecules of NFATc1 signaling pathway, which has strong relationship with cancer development. That explains us CsA has potential to explore the possibilities as a novel chemotherapeutic drug for the treatment of OSCC.

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