Proteomics analysis of plasma for potential biomarkers in the diagnosis of Alzheimer's disease

2007 ◽  
Vol 1 (5) ◽  
pp. 506-512 ◽  
Author(s):  
Pao-Chi Liao ◽  
Lung Yu ◽  
Chih-Chieh Kuo ◽  
Chingju Lin ◽  
Yu-Min Kuo
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Proteomics Analysis ◽  
Potential Biomarkers

scholarly journals Differentially charged isoforms of apolipoprotein E from human blood are potential biomarkers of Alzheimer’s disease

2014 ◽  
Vol 6 (4) ◽  
pp. 43 ◽  
Author(s):  
Oscar Alzate ◽  
Cristina Osorio ◽  
Robert M DeKroon ◽  
Ana Corcimaru ◽  
Harsha P Gunawardena
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Apolipoprotein E ◽  
Human Blood ◽  
Potential Biomarkers

scholarly journals Exosomes as possible spread factor and potential biomarkers in Alzheimer's disease: current concepts

2018 ◽  
Vol 12 (9) ◽  
pp. 1025-1033 ◽  
Author(s):  
Ryszard Pluta ◽  
Marzena Ułamek-Kozioł ◽  
Sławomir Januszewski ◽  
Stanisław J Czuczwar
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Spread Factor ◽  
Potential Biomarkers

scholarly journals Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Chunyue Wang ◽  
Xueying Cai ◽  
Ruochen Wang ◽  
Siyu Zhai ◽  
Yongfeng Zhang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Amyloid Β ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Morris Water Maze Test ◽  
Proteomics Analysis ◽  
Neuroprotective Effects ◽  
U251 Cells

Abstract Background Endoplasmic reticulum (ER) stress is involved in the progression of Alzheimer’s disease (AD). Verbascoside (VB), an active phenylethanoid glycoside that was first isolated from Verbascum sinuatum (the wavyleaf mullein), possesses anti-inflammatory, antioxidative, and anti-apoptotic effects. The purpose of this study was to elucidate the beneficial effects of VB in amyloid β (Aβ)1–42-damaged human glioma (U251) cells and in APPswe/PSEN1dE9 transgenic (APP/PS1) mice. Methods U251 cells were co-incubated with 10 μM of Aβ1-42 and treated with VB. The protective effects of VB were investigated by using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay, flow cytometry, fluorescence staining, and transmission electron microscopy. APP/PS1 transgenic mice were treated for 6 weeks with VB. Learning and memory were evaluated using a Morris water maze test. Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling, thioflavin-S staining, and proteomics analysis were performed to study the potential neuroprotective mechanism. Enzyme-linked immunosorbent assays and western blot were performed to analyze altered protein levels of brain lysates in APP/PS1 mice and/or Aβ1-42-damaged U251 cells. Results In Aβ1-42-damaged U251 cells, VB significantly improved cell viability, inhibited apoptosis, reduced calcium accumulation and the intracellular concentrations of reactive oxygen species, and improved the morphology of mitochondria and ER. In APP/PS1 mice, 6-week administration of VB significantly improved memory and cognition. VB inhibited apoptosis, reduced the deposition of Aβ, reduced the formation of neurofibrillary tangles formed by hyperphosphorylated tau protein, and downregulated the expression levels of 4-hydroxynonenal and mesencephalic astrocyte-derived neurotrophic factor in the brains of APP/PS1 mice. Proteomics analysis of mouse hippocampus suggested that the neuroprotective effect of VB may be related to the reduction of ER stress. This was indicated by the fact that VB inhibited the three branches of the unfolded protein response, thereby attenuating ER stress and preventing apoptosis. Conclusions The results confirmed that VB possesses significant neuroprotective effects, which are related to the reduction of ER stress. These findings support the status of VB as a potentially effective treatment for AD and warrant further research.

scholarly journals P3-096: Kinases mTOR and PKR controlling translation in Alzheimer's disease potential biomarkers?

2006 ◽  
Vol 2 ◽  
pp. S401-S401
Author(s):  
Marc Paccalin ◽  
Stephanie Pain Barc ◽  
Claire Paquet ◽  
Claudette Pluchon ◽  
Agnes Roiux Bilan ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Potential Biomarkers

scholarly journals Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer’s disease

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
David C. Hondius ◽  
Kristel N. Eigenhuis ◽  
Tjado H. J. Morrema ◽  
Roel C. van der Schors ◽  
Pim van Nierop ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cerebral Amyloid Angiopathy ◽  
Amyloid Angiopathy ◽  
Proteomics Analysis ◽  
Cerebral Amyloid

scholarly journals O4-02-04: EXPLORING GUT MICROBIOTA AS A SOURCE OF POTENTIAL BIOMARKERS: INITIAL RESULTS FROM THE ANAVEX® 2-73 ALZHEIMER'S DISEASE CLINICAL STUDY

2019 ◽  
Vol 15 ◽  
pp. P1232-P1233
Author(s):  
Frédéric Parmentier ◽  
Adrien Etcheto ◽  
Christopher Missling ◽  
Coralie Williams ◽  
Mohammad Afshar
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Clinical Study ◽  
Gut Microbiota ◽  
Initial Results ◽  
Potential Biomarkers
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