scholarly journals Ontogenesis of myosin light chain kinase mRNA and protein content in guinea pig tracheal smooth muscle

2004 ◽  
Vol 38 (6) ◽  
pp. 456-464 ◽  
Author(s):  
Pasquale Chitano ◽  
Judith A. Voynow ◽  
Valeria Pozzato ◽  
Viviana Cantillana ◽  
Lauranell H. Burch ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Protein Content ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Tracheal Smooth Muscle

scholarly journals Ontogenesis of myosin light chain phosphorylation in guinea pig tracheal smooth muscle

2005 ◽  
Vol 39 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Pasquale Chitano ◽  
Charles L. Worthington ◽  
Janet A. Jenkin ◽  
Newman L. Stephens ◽  
Sylvia Gyapong ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Tracheal Smooth Muscle ◽  
Myosin Light Chain Phosphorylation

GTP gamma S-dependent regulation of smooth muscle contractile elements

1992 ◽  
Vol 262 (2) ◽  
pp. C405-C410 ◽  
Author(s):  
Y. Kubota ◽  
M. Nomura ◽  
K. E. Kamm ◽  
M. C. Mumby ◽  
J. T. Stull
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Protein Phosphatase ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Kinase Activity ◽  
Tracheal Smooth Muscle ◽  
Proportional Increase ◽  
Gamma S ◽  
Muscle Contractile

Guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) increases the sensitivity of the contractile response to activation by Ca2+ in permeabilized tracheal smooth muscle. Increased tension was associated with a proportional increase in myosin light chain phosphorylation. The site of phosphorylation was determined to be serine-19, which corresponds to the site rapidly phosphorylated by myosin light chain kinase. GTP gamma S did not affect the contraction induced by the protein phosphatase inhibitor okadaic acid but did enhance contraction produced by Ca(2+)-independent myosin light chain kinase. In tracheal homogenates Ca(2+)-dependent myosin light chain kinase activity was not affected by GTP gamma S; however, dephosphorylation of 32P-labeled heavy meromyosin by phosphatase was inhibited. Thus GTP gamma S may increase the Ca2+ sensitivity of contractile elements in tracheal smooth muscle by inhibition of protein phosphatase activity toward myosin light chain.

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