Nanodiamonds Interfere with Wnt-Regulated Cell Migration and Adipocyte Differentiation in Cells and Embryonic Development In Vivo

Hongyang Yi; Xiaojiao Li; Zhuyao Wang; Min Yin; Lihua Wang; Ali Aldalbahi; Nahed Nasser El-Sayed; Hui Wang; Nan Chen; Luonan Chen; Chunhai Fan; Haiyun Song

doi:10.1002/ppsc.201600208

Nanodiamonds Interfere with Wnt-Regulated Cell Migration and Adipocyte Differentiation in Cells and Embryonic Development In Vivo

Particle & Particle Systems Characterization ◽

10.1002/ppsc.201600208 ◽

2016 ◽

Vol 34 (1) ◽

pp. 1600208 ◽

Cited By ~ 3

Author(s):

Hongyang Yi ◽

Xiaojiao Li ◽

Zhuyao Wang ◽

Min Yin ◽

Lihua Wang ◽

...

Keyword(s):

Cell Migration ◽

Embryonic Development ◽

Adipocyte Differentiation ◽

In Cells

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Cyclin B3 activates the Anaphase-Promoting Complex/Cyclosome in meiosis and mitosis

10.1101/2020.06.05.136291 ◽

2020 ◽

Author(s):

Damien Garrido ◽

Mohammed Bourouh ◽

Éric Bonneil ◽

Pierre Thibault ◽

Andrew Swan ◽

...

Keyword(s):

Embryonic Development ◽

Chromosome Segregation ◽

Ubiquitin Ligase ◽

Anaphase Promoting Complex ◽

Cells In Culture ◽

Cyclin B3 ◽

Mitosis And Meiosis ◽

In Cells

ABSTRACTIn mitosis and meiosis, chromosome segregation is triggered by the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit ubiquitin ligase that targets proteins for degradation, leading to the separation of chromatids. APC/C activation requires phosphorylation of its APC3 and APC1 subunits, which allows the APC/C to bind its Cdc20 co-activator. The identity of the kinase(s) responsible for APC/C activation in vivo is unclear. Cyclin B3 is required for meiotic anaphase in flies, worms and vertebrates, but whether it activates the APC/C is unclear. We found that Drosophila Cyclin B3 (CycB3) collaborates with PP2A-B55/Tws in embryonic development, indicating that CycB3 also promotes anaphase in mitosis. Moreover, CycB3 promotes APC/C activity and anaphase in cells in culture. We show that CycB3 physically associates with the APC/C, is required for phosphorylation of APC3, and promotes APC/C association with its co-activators. We propose that CycB3-Cdk1 directly phosphorylates the APC/C to activate it in both meiosis and mitosis.

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Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066796 ◽

1971 ◽

Vol 29 ◽

pp. 548-549

Author(s):

Awtar Krishan ◽

Dora Hsu

Keyword(s):

Granular Material ◽

Rna Synthesis ◽

Culture Medium ◽

Actinomycin D ◽

Metabolic Inhibitors ◽

Protein And Rna Synthesis ◽

Apparent Reduction ◽

Plant Alkaloids ◽

In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

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