Gold-Nanoparticle-Based Multiplexed Immunochromatographic Strip for Simultaneous Detection of Staphylococcal Enterotoxin A, B, C, D, and E

2016 ◽  
Vol 33 (7) ◽  
pp. 388-395 ◽  
Author(s):  
Wenbin Wang ◽  
Liqiang Liu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
Jianping Zhu ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Immunochromatographic Strip

Simultaneous detection of phenacetin and its metabolite using a gold nanoparticle-based immunochromatographic test strip

The Analyst ◽  
2021 ◽  
Author(s):  
Jingjing Yao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Zhengyou Wang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Test Strip ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) using an anti-PNCT monoclonal antibody (mAb). The specific anti- PNCT mAb (2D6)...

scholarly journals Gold nanoparticle-based localized surface plasmon immunosensor for staphylococcal enterotoxin A (SEA) detection

2017 ◽  
Vol 409 (26) ◽  
pp. 6227-6234 ◽  
Author(s):  
Maroua Ben Haddada ◽  
David Hu ◽  
Michèle Salmain ◽  
Lu Zhang ◽  
Chen Peng ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Staphylococcal Enterotoxin ◽  
Localized Surface Plasmon ◽  
Staphylococcal Enterotoxin A

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

2019 ◽  
Vol 13 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Tzonka Godjevargova ◽  
Zlatina Becheva ◽  
Yavor Ivanov ◽  
Andrey Tchorbanov
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Fluorescence Immunoassay ◽  
Magnetic Separator ◽  
Milk Samples

Objectives: Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods: Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results: The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion: A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

scholarly journals Rapid and simultaneous detection of ricin, staphylococcal enterotoxin B and saxitoxin by chemiluminescence-based microarray immunoassay

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5885-5892 ◽  
Author(s):  
A. Szkola ◽  
E. M. Linares ◽  
S. Worbs ◽  
B. G. Dorner ◽  
R. Dietrich ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Microarray Immunoassay ◽  
Enterotoxin B ◽  
Idiotypic Antibody

Simultaneous detection of proteotoxins, ricin and SEB, and small toxin, STX, on a chemiluminescence-based microarray using anti-idiotypic antibody for STX.

Gold nanoparticle-decorated MoS2 nanosheets for simultaneous detection of ascorbic acid, dopamine and uric acid

RSC Advances ◽  
2014 ◽  
Vol 4 (52) ◽  
pp. 27625 ◽  
Author(s):  
Haofan Sun ◽  
Jie Chao ◽  
Xiaolei Zuo ◽  
Shao Su ◽  
Xingfen Liu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Gold Nanoparticle ◽  
Simultaneous Detection ◽  
Mos2 Nanosheets

scholarly journals Mass Outbreak of Food Poisoning Disease Caused by Small Amounts of Staphylococcal Enterotoxins A and H

2005 ◽  
Vol 71 (5) ◽  
pp. 2793-2795 ◽  
Author(s):  
Tetsuya Ikeda ◽  
Naoto Tamate ◽  
Keiji Yamaguchi ◽  
Sou-ichi Makino
Keyword(s):  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Raw Material ◽  
Staphylococcal Enterotoxin A ◽  
Staphylococcal Enterotoxins ◽  
Reconstituted Milk

ABSTRACT It was believed that food poisoning in Osaka in 2000 was due to small amounts of staphylococcal enterotoxin A (SEA) in reconstituted milk. Results of this study clearly indicate that SEH was also present in the raw material of reconstituted milk, indicating that the food poisoning was caused by multiple staphylococcal enterotoxins.

scholarly journals Production of Staphylococcal Enterotoxin A in Blue, Brick, Mozzarella, and Swiss Cheeses

1973 ◽  
Vol 56 (4) ◽  
pp. 429-435 ◽  
Author(s):  
S.R. Tatini ◽  
W.D. Wesala ◽  
J.J. Jezeski ◽  
H.A. Morris
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A
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