Toward defining plasma treatment dose: The role of plasma treatment energy of pulsed‐dielectric barrier discharge in dictating in vitro biological responses

2021 ◽  
Author(s):  
Abraham Lin ◽  
Eline Biscop ◽  
Yury Gorbanev ◽  
Evelien Smits ◽  
Annemie Bogaerts
Keyword(s):  
Plasma Treatment ◽  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Dielectric Barrier ◽  
Biological Responses ◽  
Treatment Dose

scholarly journals Dielectric barrier discharge plasma treatment of modified SU-8 for biosensing applications

2018 ◽  
Vol 9 (5) ◽  
pp. 2168 ◽  
Author(s):  
Jhonattan C. Ramirez ◽  
Juliana N. Schianti ◽  
Denio E. P. Souto ◽  
Lauro T. Kubota ◽  
Hugo E. Hernandez-Figueroa ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Dielectric Barrier Discharge ◽  
Discharge Plasma ◽  
Barrier Discharge ◽  
Dielectric Barrier Discharge Plasma ◽  
Dielectric Barrier ◽  
Barrier Discharge Plasma

scholarly journals Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

2020 ◽  
Vol 21 (17) ◽  
pp. 6326
Author(s):  
Akikazu Sakudo ◽  
Tatsuya Misawa
Keyword(s):  
Plasma Treatment ◽  
Cell Count ◽  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Viable Cell ◽  
Resistant Bacteria ◽  
Free Medium ◽  
Antibiotic Resistant ◽  
Dielectric Barrier ◽  
E Coli

Here, we examined whether antibiotic-resistant and non-resistant bacteria show a differential susceptibility to plasma treatment. Escherichia coli DH5α were transformed with pPRO-EX-HT-CAT, which encodes an ampicillin resistance gene and chloramphenicol acetyltransferase (CAT) gene, and then treated with a dielectric barrier discharge (DBD) plasma torch. Plasma treatment reduced the viable cell count of E. coli after transformation/selection and further cultured in ampicillin-containing and ampicillin-free medium. However, there was no significant difference in viable cell count between the transformed and untransformed E. coli after 1 min- and 2 min-plasma treatment. Furthermore, the enzyme-linked immunosorbent assay (ELISA) and acetyltransferase activity assay showed that the CAT activity was reduced after plasma treatment in both transformed and selected E. coli grown in ampicillin-containing or ampicillin-free medium. Loss of lipopolysaccharide and DNA damage caused by plasma treatment were confirmed by a Limulus test and polymerase chain reaction, respectively. Taken together, these findings suggest the plasma acts to degrade components of the bacteria and is therefore unlikely to display a differential affect against antibiotic-resistant and non-resistant bacteria. Therefore, the plasma method may be useful in eliminating bacteria that are recalcitrant to conventional antibiotic therapy.

scholarly journals Virucidal Effects of Dielectric Barrier Discharge Plasma on Human Norovirus Infectivity in Fresh Oysters (Crassostrea gigas)

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1731
Author(s):  
Man-Seok Choi ◽  
Eun Bi Jeon ◽  
Ji Yoon Kim ◽  
Eun Ha Choi ◽  
Jun Sup Lim ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Dielectric Barrier Discharge ◽  
Copy Number ◽  
Barrier Discharge ◽  
Quantitative Polymerase Chain Reaction ◽  
Inactivation Kinetics ◽  
Dbd Plasma ◽  
Human Norovirus ◽  
Dielectric Barrier ◽  
The Impact

This study investigates the effects of dielectric barrier discharge (DBD) plasma treatment (1.1 kV, 43 kHz, N2 1.5 L/min, 10~60 min) on human norovirus (HuNoV) GII.4 infectivity in fresh oysters. HuNoV viability in oysters was assessed by using propidium monoazide (PMA) as a nucleic acid intercalating dye before performing a real-time reverse transcription–quantitative polymerase chain reaction (RT-qPCR). Additionally, the impact of the DBD plasma treatment on pH and Hunter colors was assessed. When DBD plasma was treated for 60 min, the HuNoV genomic titer reduction without PMA pretreatment was negligible (<1 log copy number/µL), whereas when PMA treatment was used, HuNoV titer was reduced to >1 log copy number/µL in just 30 min. D1 and D2-value of HuNoV infectivity were calculated as 36.5 and 73.0 min of the DBD plasma treatment, respectively, using the first-order kinetics model (R2 = 0.98). The pH and Hunter colors were not significantly different (p > 0.05) between the untreated and DBD-plasma-treated oysters. The results suggest that PMA/RT-qPCR could help distinguish HuNoV infectivity without negatively affecting oyster quality following >30 min treatment with DBD plasma. Moreover, the inactivation kinetics of nonthermal DBD plasma against HuNoV in fresh oysters might provide basic information for oyster processing and distribution.

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