Seed treatment with cold plasma and electromagnetic field induces changes in red clover root growth dynamics, flavonoid exudation, and activates nodulation

2020 ◽  
Author(s):  
Vida Mildaziene ◽  
Anatolii Ivankov ◽  
Giedre Pauzaite ◽  
Zita Naucienė ◽  
Rasa Zukiene ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Root Growth ◽  
Seed Treatment ◽  
Cold Plasma ◽  
Growth Dynamics ◽  
Red Clover ◽  
Clover Root

Effect of seed treatment with cold plasma and electromagnetic field on red clover germination, growth and content of major isoflavones

2020 ◽  
Vol 53 (26) ◽  
pp. 264001 ◽  
Author(s):  
Vida Mildažienė ◽  
Giedrė Paužaitė ◽  
Zita Naučienė ◽  
Rasa Žūkienė ◽  
Asta Malakauskienė ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Seed Treatment ◽  
Cold Plasma ◽  
Red Clover

scholarly journals The Effects of Red Clover Seed Treatment with Cold Plasma and Electromagnetic Field on Germination and Seedling Growth Are Dependent on Seed Color

2021 ◽  
Vol 11 (10) ◽  
pp. 4676
Author(s):  
Anatolii Ivankov ◽  
Rasa Zukiene ◽  
Zita Nauciene ◽  
Laima Degutyte-Fomins ◽  
Irina Filatova ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Cold Plasma ◽  
Trifolium Pratense ◽  
Root Nodules ◽  
Germination Rate ◽  
Red Clover ◽  
Seed Color ◽  
Seed Treatments ◽  
Capacitively Coupled ◽  
Clover Seed

This study aimed to estimate the effects of cold plasma (CP) and electromagnetic field (EMF) treatment of red clover (Trifolium pratense) seeds with different coat colors on germination kinetics, the content of seed phytohormones, and the growth of seedlings. Seeds of red clover cultivar ‘Arimaiciai’ were treated with radio-frequency EMF or capacitively coupled low-pressure CP for different durations. There were no differences in germination kinetics between yellow, brown, and dark purple seeds in control, but the germination rate of seeds treated with CP and EMF depended on seed color: The germination of yellow seeds was stimulated stronger compared to dark purple and brown seeds, and EMF did not stimulate germination in brown seeds. The content of phytohormones in control seeds and the shift in their amount induced by seed treatments were also strongly dependent on seed color. No relationship was found between the effect on germination kinetics and changes in phytohormone levels. In the control, seedlings growing from the yellow seeds were heavier, and the number of root nodules was 12.5 times larger compared to seedlings of dark purple seeds. Seed treatments with CP and EMF significantly increased the number of root nodules, and this effect was stronger in seedlings from dark purple seeds compared to those from yellow seeds.

scholarly journals Changes in Growth and Production of Non-Psychotropic Cannabinoids Induced by Pre-Sowing Treatment of Hemp Seeds with Cold Plasma, Vacuum and Electromagnetic Field

2020 ◽  
Vol 10 (23) ◽  
pp. 8519
Author(s):  
Anatolii Ivankov ◽  
Zita Nauciene ◽  
Rasa Zukiene ◽  
Laima Degutyte-Fomins ◽  
Asta Malakauskiene ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Seed Treatment ◽  
Cold Plasma ◽  
Vacuum Treatment ◽  
Plant Production ◽  
Hemp Seed ◽  
Cannabidiolic Acid ◽  
Industrial Hemp ◽  
Ground Part

In this study, the effects of seed treatments with different stressors, such as cold plasma (CP), a vacuum and an electromagnetic field (EMF), on the in vitro germination of industrial hemp cv. Futura 75 were compared with the effects on germination in the field, plant growth, and the amount of major cannabinoids in the leaves and inflorescences of female plants. CP and EMF (but not vacuum) treatments improved in vitro seed germination, but had no impact on germination in the field. EMF treatment increased the weight of the above-ground part of male and female plants grown for 4 months by 65–70% and the number of female inflorescences by 70%. CP stimulated the growth of male plants (weight increased 1.4 times) but reduced the growth of female plants. Vacuum treatment did not induce changes in the growth of female and male plants. Vacuum and EMF treatments did not change the amount of cannabidiolic acid (CBDA), but CP decreased the CBDA content in hemp leaves by 41%. Vacuum treatment increased the amount of CBDA in female plant inflorescences by 26%. Thus, hemp seed treatment with EMF has a potential application for increasing the biomass of female plants. CP treatment can be used to increase male plant production while vacuum treatment can stimulate CBD production.

scholarly journals Changes in Agricultural Performance of Common Buckwheat Induced by Seed Treatment with Cold Plasma and Electromagnetic Field

2021 ◽  
Vol 11 (10) ◽  
pp. 4391
Author(s):  
Anatolii Ivankov ◽  
Zita Naučienė ◽  
Laima Degutytė-Fomins ◽  
Rasa Žūkienė ◽  
Irena Januškaitienė ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Plant Growth ◽  
Biomass Production ◽  
Seed Yield ◽  
Seed Treatment ◽  
Cold Plasma ◽  
Common Buckwheat ◽  
Seed Treatments ◽  
Plant Seed ◽  
Agricultural Performance

The aim of this study was to determine the effects of pre-sowing seed treatment with cold plasma (CP) and an electromagnetic field (EMF) on the agricultural performance of two cultivars of common buckwheat (Fagopyrum esculentum Moench)—‘VB Vokiai’ and ‘VB Nojai’. For this, the effects of CP and EMF on seed germination, plant growth in the field, photosynthetic efficiency, biomass production, seed yield, and the amount of secondary metabolites and minerals in the harvested seeds were estimated. Although the percentage of seedlings that emerged under field conditions decreased by 11–20%, seed treatments strongly improved buckwheat growth and yield. Irrespective of differences in the dynamics of changes in the growth and photosynthetic activity between the two cultivars, the weight of seeds collected per plant for both cultivars was significantly higher (up to 70–97%) compared to the control. The biochemical composition of the harvested seeds (Fe, Zn, quercetin content) was also altered by seed treatments. Thus, pre-sowing treatment of buckwheat seeds with CP and EMF substantially stimulated plant growth in the field, increased biomass production, seed yield and nutritional quality. The results obtained strongly support the idea that plant seed treatment with physical stressors has great potential for use in agriculture.

Genetic variation for rate of establishment in caucasian clover

1998 ◽  
pp. 213-217
Author(s):  
K.H. Widdup ◽  
T.L. Knight ◽  
C.J. Waters
Keyword(s):  
Genetic Variation ◽  
Root Growth ◽  
Seedling Growth ◽  
White Clover ◽  
Seedling Emergence ◽  
Red Clover ◽  
Dry Weight ◽  
Cool Season ◽  
Trifolium Ambiguum ◽  
Root Dry Weight

Slow establishment of caucasian clover (Trifolium ambiguum L.) is hindering the use of this legume in pasture mixtures. Improved genetic material is one strategy of correcting the problem. Newly harvested seed of hexaploid caucasian clover germplasm covering a range of origins, together with white and red clover and lucerne, were sown in 1 m rows in a Wakanui soil at Lincoln in November 1995. After 21 days, the caucasian clover material as a group had similar numbers of emerged seedlings as white clover and lucerne, but was inferior to red clover. There was wide variation among caucasian clover lines (48-70% seedling emergence), with the cool-season selection from cv. Monaro ranked the highest. Recurrent selection at low temperatures could be used to select material with improved rates of seedling emergence. Red clover and lucerne seedlings produced significantly greater shoot and root dry weight than caucasian and white clover seedlings. Initially, caucasian clover seedlings partitioned 1:1 shoot to root dry weight compared with 3:1 for white clover. After 2 months, caucasian clover seedlings had similar shoot growth but 3 times the root growth of white clover. Between 2 and 5 months, caucasian clover partitioned more to root and rhizome growth, resulting in a 0.3:1 shoot:root ratio compared with 2:1 for white clover. Both clover species had similar total dry weight after 5 months. Unhindered root/ rhizome devel-opment is very important to hasten the establishment phase of caucasian clover. The caucasian clover lines KZ3 and cool-season, both selections from Monaro, developed seedlings with greater shoot and root growth than cv. Monaro. KZ3 continued to produce greater root growth after 5 months, indicating the genetic potential for improvement in seedling growth rate. Different pasture estab-lishment techniques are proposed that take account of the seedling growth characteristics of caucasian clover. Keywords: establishment, genetic variation, growth, seedling emergence, Trifolium ambiguum

Utilising cold plasma seed treatment technologies to delay cotyledon senescence in tomato seedlings

2021 ◽  
Vol 281 ◽  
pp. 109911
Author(s):  
Kai Li ◽  
Lili Zhang ◽  
Changyong Shao ◽  
Chongshan Zhong ◽  
Bili Cao ◽  
...  
Keyword(s):  
Seed Treatment ◽  
Cold Plasma ◽  
Tomato Seedlings ◽  
Treatment Technologies ◽  
Cotyledon Senescence

scholarly journals Lateral Root Priming Synergystically Arises from Root Growth and Auxin Transport Dynamics

2018 ◽  
Author(s):  
Thea van den Berg ◽  
Kirsten H. ten Tusscher
Keyword(s):  
Cell Cycle ◽  
Root Growth ◽  
Lateral Root ◽  
Root Formation ◽  
Growth Dynamics ◽  
Root Tip ◽  
Cycle Duration ◽  
Elongation Zone ◽  
Lateral Root Formation ◽  
Cell Cycle Duration

AbstractThe root system is a major determinant of plant fitness. Its capacity to supply the plant with sufficient water and nutrients strongly depends on root system architecture, which arises from the repeated branching off of lateral roots. A critical first step in lateral root formation is priming, which prepatterns sites competent of forming a lateral root. Priming is characterized by temporal oscillations in auxin, auxin signalling and gene expression in the root meristem, which through growth become transformed into a spatially repetitive pattern of competent sites. Previous studies have demonstrated the importance of auxin synthesis, transport and perception for the amplitude of these oscillations and their chances of producing an actual competent site. Additionally, repeated lateral root cap apoptosis was demonstrated to be strongly correlated with repetitive lateral root priming. Intriguingly, no single mutation has been identified that fully abolishes lateral root formation, and thusfar the mechanism underlying oscillations has remained unknown. In this study, we investigated the impact of auxin reflux loop properties combined with root growth dynamics on priming, using a computational approach. To this end we developed a novel multi-scale root model incorporating a realistic root tip architecture and reflux loop properties as well as root growth dynamics. Excitingly, in this model, repetitive auxin elevations automatically emerge. First, we show that root tip architecture and reflux loop properties result in an auxin loading zone at the start of the elongation zone, with preferential auxin loading in narrow vasculature cells. Second, we demonstrate how meristematic root growth dynamics causes regular alternations in the sizes of cells arriving at the elongation zone, which subsequently become amplified during cell expansion. These cell size differences translate into differences in cellular auxin loading potential. Combined, these properties result in temporal and spatial fluctuations in auxin levels in vasculature and pericycle cells. Our model predicts that temporal priming frequency predominantly depends on cell cycle duration, while cell cycle duration together with meristem size control lateral root spacing.

scholarly journals Biological Effects of a Low-Frequency Electromagnetic Field on Yeast Cells of the Genus Saccharomyces Cerevisiae

2021 ◽  
Vol 21 (2) ◽  
pp. 34-41
Author(s):  
K Sladicekova ◽  
M Bereta ◽  
J Misek ◽  
D Parizek ◽  
J Jakus
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Electromagnetic Field ◽  
Magnetic Flux ◽  
Flux Density ◽  
Biological Effects ◽  
Low Frequency ◽  
Growth Dynamics ◽  
Yeast Cells ◽  
Magnetic Flux Density ◽  
Time Points

Abstract Background: Although the scientific community is extensively concerned with the effects of the EMF, the unambiguous explanation of its effects on living structures is still lacking. Goals: The goal of the study was to evaluate the effect of a low-frequency (LF) electromagnetic field (EMF) on the growth and multiplication of the yeast Saccharomyces cerevisiae. Methods: Yeast cells were exposed to a frequency of 900 Hz and a magnetic flux density of 2.3 mT. The duration of each experiment was 8 hours, in the beginning of the measurement the value of frequency, rms (root mean square) value of electric current (2 A), and magnetic flux density were fixed set on the exposure device. A paired experiment was performed, a sample exposed to EMF, and a sample shielded from the field. Subsequently, samples were taken every two hours, the number of cells was recorded, and then the concentration of the yeast cells was evaluated at time points. The time points reflected the exposure time of the samples exposed to EMF. Results: The results indicate that LF EMF at given parameters has an inhibitory effect on the growth and multiplication of yeast cells. Conclusion: Exposure to EMF can cause the differences in growth dynamics between cells exposed to the field and the unexposed ones.

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