Effects of Low‐Temperature Atmospheric Air Plasmas on the Activity and Function of Human Lymphocytes

2008 ◽  
Vol 5 (5) ◽  
pp. 482-488 ◽  
Author(s):  
Xing‐Min Shi ◽  
Guan‐Jun Zhang ◽  
Yu‐Kang Yuan ◽  
Yue Ma ◽  
Gui‐Min Xu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Human Lymphocytes ◽  
Atmospheric Air ◽  
Air Plasmas ◽  
And Function

The krypton and xenon contents of atmospheric air

1956 ◽  
Vol 234 (1199) ◽  
pp. 557-565 ◽  
Keyword(s):  
Gas Chromatography ◽  
Low Temperature ◽  
Atmospheric Air ◽  
Dry Air

The proportion of krypton and xenon in dry air has been measured by a method using distillation and low-temperature gas-chromatography for the isolation of krypton and xenon. The separation has been controlled by the use of radioactive 85 Kr tracer. The krypton content of dry air is 1⋅139 ± 0⋅01 x 10 -6 by volume. The xenon content of dry air is 0⋅086 ± 0⋅001 x 10 -6 by volume.

scholarly journals Simple, compact source for low-temperature air plasmas

2002 ◽  
Vol 73 (8) ◽  
pp. 3128-3130
Author(s):  
D. P. Sheehan ◽  
J. Lawson ◽  
M. Sosa ◽  
R. A. Long
Keyword(s):  
Low Temperature ◽  
Compact Source ◽  
Air Plasmas

Expression and Function of Non-neuronal GABA Transporters in Human Lymphocytes

2017 ◽  
Vol 13 (2) ◽  
pp. 205-211
Author(s):  
L. Dionisio ◽  
H. Caldironi ◽  
M.J. De Rosa
Keyword(s):  
Human Lymphocytes ◽  
Gaba Transporters ◽  
And Function

scholarly journals Efficient Assembly of Ribosomes Is Inhibited by Deletion ofbipAin Escherichia coli

2015 ◽  
Vol 197 (10) ◽  
pp. 1819-1827 ◽  
Author(s):  
Promisree Choudhury ◽  
Ann M. Flower
Keyword(s):  
Low Temperature ◽  
Modified Nucleosides ◽  
Cold Sensitivity ◽  
23S Rrna ◽  
Ribosome Assembly ◽  
Rrna Processing ◽  
Pseudouridine Synthase ◽  
Assembly Factor ◽  
Cold Sensitive ◽  
And Function

ABSTRACTThe bacterial BipA protein belongs to the EF-G family of translational GTPases and has been postulated to be either a regulatory translation factor or a ribosome assembly factor. To distinguish between these hypotheses, we analyzed the effect ofbipAdeletion on three phenotypes associated with ribosome assembly factors: cold sensitivity, ribosome subunit distribution, and rRNA processing. We demonstrated that a ΔbipAstrain exhibits a cold-sensitive phenotype that is similar to, and synergistic with, that of a strain with a known ribosome assembly factor,deaD. Additionally, thebipAdeletion strain displayed a perturbed ribosome subunit distribution when grown at low temperature, similar to that of adeaDmutant, and again, the double mutant showed additive effects. The primary ribosomal deficiency noted was a decreased level of the 50S subunit and the appearance of a presumed pre-50S particle. Finally, deletion ofbipAresulted in accumulation of pre23S rRNA, as did deletion ofdeaD. We further found that deletion ofrluC, which encodes a pseudouridine synthase that modifies the 23S rRNA at three sites, suppressed all three phenotypes of thebipAmutant, supporting and extending previous findings. Together, these results suggest that BipA is important for the correct and efficient assembly of the 50S subunit of the ribosome at low temperature but when unmodified by RluC, the ribosomes become BipA independent for assembly.IMPORTANCEThe ribosome is the complex ribonucleoprotein machine responsible for protein synthesis in all cells. Although much has been learned about the structure and function of the ribosome, we do not fully understand how it is assembled or the accessory proteins that increase efficiency of biogenesis and function. This study examined one such protein, BipA. Our results indicate that BipA either directly or indirectly enhances the formation of the 50S subunit of the ribosome, particularly at low temperature. In addition, ribosomes contain a large number of modified nucleosides, including pseudouridines. This work demonstrates that the function of BipA is tied to the modification status of the ribosome and may help us understand why these modifications have been retained.

scholarly journals A determination of the amounts of neon and helium in atmospheric air

1905 ◽  
Vol 76 (508) ◽  
pp. 111-114 ◽  
Keyword(s):  
Low Temperature ◽  
Vapour Pressure ◽  
Atmospheric Air ◽  
Appreciable Quantity ◽  
Ingenious Method ◽  
James Dewar ◽  
Very Low Temperature

Some time ago I communicated to the Society the results of an attempt to estimate the amounts of krypton and of xenon in air. The quantities were necessarily minimum estimates, for there is no doubt that both krypton and xenon must evaporate when air evaporates, even if that take place at a very low temperature. Dr. Travers and I guessed at the amounts of neon and helium, and supposed that the amount of helium was one or two parts per million, and that of neon one or two parts per 100,000. This guess is not very far from the truth, as the following account of recent experiments will show. The ingenious method discovered by Sir James Dewar of using cooled cocoanut charcoal as an absorbent for gases has made it easy to carry out the estimation. The process consists in cooling 100 grammes of such charcoal to — 100°, approximately, in a bulb from which all air has been removed by a pump. Such charcoal will absorb about three litres of air; at that temperature neither neon nor helium are absorbed in appreciable quantity, as special experiments showed. Hence on placing the cooled bulb containing the char­coal in communication with a Töpler pump, the uncondensed gases enter the barrel of the pump. On closing the connecting stop-cock, a further quantity of gas accumulates, and is again removed into the pump in the same manner. As the relative volumes of the pump and of the cooled bulb were approxi­mately 4 to 1, after communication had been established four times, only 1/256 of the contents of the bulb were left in it. And as the gas in contact with charcoal exerts a kind of vapour-pressure, inasmuch as the pressure which it gives depends on the temperature as well as on the extent of the surface of charcoal to which it is exposed, it may be assumed that gas escapes from the charcoal on each reduction of pressure, and that the more volatile gases in the bulb should be expelled by the less volatile.

Products and mechanisms of the oxidation of organic compounds in atmospheric air plasmas

2010 ◽  
Vol 43 (12) ◽  
pp. 124011 ◽  
Author(s):  
Ester Marotta ◽  
Milko Schiorlin ◽  
Massimo Rea ◽  
Cristina Paradisi
Keyword(s):  
Organic Compounds ◽  
Atmospheric Air ◽  
Air Plasmas ◽  
Oxidation Of Organic Compounds

scholarly journals Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A

Cells ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 136
Author(s):  
Miquéias Lopes-Pacheco ◽  
Mafalda Bacalhau ◽  
Sofia S. Ramalho ◽  
Iris A. L. Silva ◽  
Filipa C. Ferreira ◽  
...  
Keyword(s):  
Low Temperature ◽  
Small Molecules ◽  
Mechanism Of Action ◽  
Immunofluorescence Microscopy ◽  
Additive Effects ◽  
Wild Type ◽  
Functional Assays ◽  
And Function ◽  
Putative Binding Site ◽  
Shed Light

Although some therapeutic progress has been achieved in developing small molecules that correct F508del-CFTR defects, the mechanism of action (MoA) of these compounds remain poorly elucidated. Here, we investigated the effects and MoA of MCG1516A, a newly developed F508del-CFTR corrector. MCG1516A effects on wild-type (WT) and F508del-CFTR were assessed by immunofluorescence microscopy, and biochemical and functional assays both in cell lines and in intestinal organoids. To shed light on the MoA of MCG1516A, we evaluated its additivity to the FDA-approved corrector VX-661, low temperature, genetic revertants of F508del-CFTR (G550E, R1070W, and 4RK), and the traffic-null variant DD/AA. Finally, we explored the ability of MCG1516A to rescue trafficking and function of other CF-causing mutations. We found that MCG1516A rescues F508del-CFTR with additive effects to VX-661. A similar behavior was observed for WT-CFTR. Under low temperature incubation, F508del-CFTR demonstrated an additivity in processing and function with VX-661, but not with MCG1516A. In contrast, both compounds promoted additional effects to low temperature to WT-CFTR. MCG1516A demonstrated additivity to genetic revertant R1070W, while VX-661 was additive to G550E and 4RK. Nevertheless, none of these compounds rescued DD/AA trafficking. Both MCG1516A and VX-661 rescued CFTR processing of L206W- and R334W-CFTR with greater effects when these compounds were combined. In summary, the absence of additivity of MCG1516A to genetic revertant G550E suggests a putative binding site for this compound on NBD1:NBD2 interface. Therefore, a combination of MCG1516A with compounds able to rescue DD/AA traffic, or mimicking the actions of revertant R1070W (e.g., VX-661), could enhance correction of F508del-CFTR defects.

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