Optimized peptide functionalization of thiol‐acrylate emulsion‐templated porous polymers leads to expansion of human pluripotent stem cells in 3D culture

2019 ◽  
Vol 57 (18) ◽  
pp. 1974-1981 ◽  
Author(s):  
Jessie L. Ratcliffe ◽  
Marc Walker ◽  
Ahmed M. Eissa ◽  
Shengrong Du ◽  
Stefan A. Przyborski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Porous Polymers ◽  
Acrylate Emulsion ◽  
Peptide Functionalization

scholarly journals Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

2015 ◽  
Vol 12 (7) ◽  
pp. 671-678 ◽  
Author(s):  
Anca M Paşca ◽  
Steven A Sloan ◽  
Laura E Clarke ◽  
Yuan Tian ◽  
Christopher D Makinson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cortical Neurons ◽  
3D Culture ◽  
Human Pluripotent Stem Cells

scholarly journals Characterization of Phenotypic and Transcriptional Differences in Human Pluripotent Stem Cells under 2D and 3D Culture Conditions

2016 ◽  
Vol 5 (22) ◽  
pp. 2951-2958 ◽  
Author(s):  
Ken-ichiro Kamei ◽  
Yoshie Koyama ◽  
Yumie Tokunaga ◽  
Yasumasa Mashimo ◽  
Momoko Yoshioka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Culture Conditions ◽  
Human Pluripotent Stem Cells ◽  
2D And 3D

scholarly journals Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

Cell Reports ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. 537-550 ◽  
Author(s):  
Keiko Muguruma ◽  
Ayaka Nishiyama ◽  
Hideshi Kawakami ◽  
Kouichi Hashimoto ◽  
Yoshiki Sasai
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Self Organization ◽  
Cerebellar Tissue

scholarly journals Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ulf Diekmann ◽  
Hanna Wolling ◽  
Rabea Dettmer ◽  
Isabell Niwolik ◽  
Ortwin Naujok ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Definitive Endoderm

scholarly journals Assessment of the Hematopoietic Differentiation Potential of Human Pluripotent Stem Cells in 2D and 3D Culture Systems

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2858
Author(s):  
German Atzin Mora-Roldan ◽  
Dalia Ramirez-Ramirez ◽  
Rosana Pelayo ◽  
Karlen Gazarian
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Time Course ◽  
Hematopoietic Cells ◽  
3D Culture ◽  
Human Pluripotent Stem Cells ◽  
Hematopoietic Differentiation ◽  
Differentiation Potential ◽  
2D And 3D

Background. In vitro methods for hematopoietic differentiation of human pluripotent stem cells (hPSC) are a matter of priority for the in-depth research into the mechanisms of early embryogenesis. So-far, published results regarding the generation of hematopoietic cells come from studies using either 2D or 3D culture formats, hence, it is difficult to discern their particular contribution to the development of the concept of a unique in vitro model in close resemblance to in vivo hematopoiesis. Aim of the study. To assess using the same culture conditions and the same time course, the potential of each of these two formats to support differentiation of human pluripotent stem cells to primitive hematopoiesis without exogenous activation of Wnt signaling. Methods. We used in parallel 2D and 3D formats, the same culture environment and assay methods (flow cytometry, IF, qPCR) to investigate stages of commitment and specification of mesodermal, and hemogenic endothelial cells to CD34 hematopoietic cells and evaluated their clonogenic capacity in a CFU system. Results. We show an adequate formation of mesoderm, an efficient commitment to hemogenic endothelium, a higher number of CD34 hematopoietic cells, and colony-forming capacity potential only in the 3D format-supported differentiation. Conclusions. This study shows that the 3D but not the 2D format ensures the induction and realization by endogenous mechanisms of human pluripotent stem cells’ intrinsic differentiation program to primitive hematopoietic cells. We propose that the 3D format provides an adequate level of upregulation of the endogenous Wnt/β-catenin signaling.

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