Correction of the mark–houwink–sakurada equation in the low-molecular-weight range

1985 ◽  
Vol 23 (4) ◽  
pp. 615-621 ◽  
Author(s):  
A. Dondos ◽  
V. Skordilis
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Molecular Weight Range ◽  
Weight Range

Identification of luteinizing hormone receptor binding inhibitor in bovine corpora lutea

1995 ◽  
Vol 132 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Premila Rathnam ◽  
Shou-qing Lin ◽  
Brij B Saxena
Keyword(s):  
Molecular Weight ◽  
Luteinizing Hormone ◽  
Receptor Binding ◽  
Hormone Receptor ◽  
Luteinizing Hormone Receptor ◽  
Low Molecular Weight ◽  
Corpora Lutea ◽  
Molecular Weight Range ◽  
Lh Receptor ◽  
Weight Range

Rathnam P, Lin S, Saxena BB. Identification of luteinizing hormone receptor binding inhibitor in bovine corpora lutea. Eur J Endocrinol 1995;132:213–17. ISSN 0804–4643 A 7000 g supernatant, obtained during the purification of luteinizing hormone (LH) receptor from bovine corpora lutea homogenate, was concentrated by ultrafiltration. The filtrate, containing < 50 000 molecular weight material, exhibited LH receptor binding inhibitor (LH-RBI) activity. The filtrate was ultrafiltered sequentially through Amicon PM-10, PM-30 and UM-2 filters to yield a LH-RBI-containing fraction in the higher molecular weight range of 30 000–10 000 and a LH-RBI-containing fraction in the lower molecular weight range of 10 000–1000. The higher molecular weight LH-RBI fraction was purified on Sephadex G-25 and the lower molecular weight LH-RBI fraction was purified on Sephadex G-50. Both the high- and the low-molecular-weight LH-RBI species inhibited the binding of 125I-labeled human chorionic gonadotropin (hCG) to bovine corpora lutea and to rat Leydig cell membrane receptors. Similarly, the production of testosterone by hCG-stimulated rat Leydig cells was inhibited in a dose–response manner by both the high- and the low-molecular-weight LH-RBI species. The LH-RBI activity in the low-molecular-weight species was stable at 4°C for up to 6 months and at temperatures up to 90°C for 15 mins, whereas the LH-RBI activity of the high-molecular-weight species was stable at 4°C for 15 months and unstable at 60°C after 15 min. The 7000 g supernatant provided a much-needed source to obtain larger than previously reported quantities of LH-RBI for isolation as well as for structure and function studies. Brij B Saxena, Cornell Univ. Medical College, Rm A-267, 1300 York Ave, New York, NY 10021, USA

Partial purification and characterization ofGigantocotyle explanatumsomatic antigens

2004 ◽  
Vol 78 (2) ◽  
pp. 95-99 ◽  
Author(s):  
G. Ahmad ◽  
M.K. Saifullah ◽  
W.A. Nizami
Keyword(s):  
Molecular Weight ◽  
Western Blotting ◽  
Bubalus Bubalis ◽  
Partial Purification ◽  
Low Molecular Weight ◽  
Purification And Characterization ◽  
Molecular Weight Range ◽  
Weight Range ◽  
Sds Page

AbstractSoluble extracts ofGigantocotyle explanatum, isolated from the liver of buffaloBubalus bubaliswere fractionated on Sephadex G-200 columns. Nine major fractions referred to as F1, F2, F3, F4, F5, F6, F7, F8 and F9 were separated. Each fraction was tested by ELISA for antigenicity using sera fromG. explanatum-infected field buffaloes. Fractions F1 and F2 were highly antigenic, F3, F4, F6 and F7 were moderately antigenic and F5, F8 and F9 were poorly antigenic. Analyses by SDS–PAGE revealed that each fraction comprised several polypeptide(s) in the molecular weight range of <29 to >205 kDa. Results of Western blotting indicated that not all polypeptides which appeared in the SDS–PAGE were antigenic. The antigenic molecules of each fraction were mostly in the low molecular weight range of <14 to >94 kDa with the polypeptides in the range of >14, 14, 18, 21–25 and 34–36 kDa.

scholarly journals Nuclear matrix of HeLa S3 cells. Polypeptide composition during adenovirus infection and in phases of the cell cycle.

1977 ◽  
Vol 72 (1) ◽  
pp. 194-208 ◽  
Author(s):  
L D Hodge ◽  
P Mancini ◽  
F M Davis ◽  
P Heywood
Keyword(s):  
Cell Cycle ◽  
Molecular Weight ◽  
Nuclear Matrix ◽  
Apparent Molecular Weight ◽  
Molecular Weight Range ◽  
Weight Range ◽  
Molecular Weights ◽  
Liver Nuclei ◽  
Hela S3 ◽  
Biochemical Analyses

A subnuclear fraction has been isolated from HeLa S3 nuclei after treatment with high salt buffer, deoxyribonuclease, and dithiothreitol. This fraction retains the approximate size and shape of nuclei and resembles the nuclear matrix recently isolated from rat liver nuclei. Ultrastructural and biochemical analyses indicate that this structure consists of nonmembranous elements as well as some membranous elements. Its chemical composition is 87% protein, 12% phospholipid, 1% DNA, and 0.1% RNA by weight. The protein constituents are resolved in SDS-polyacrylamide slab gels into 30-35 distinguishable bands in the apparent molecular weight range of 14,000 - 200,000 with major peptides at 14,000 - 18,000 and 45,000 - 75,000. Analysis of newly synthesized polypeptides by cylindrical gel electrophoresis reveals another cluster in the 90,000-130,000 molecular weight range. Infection with adenovirus results in an altered polypeptide profile. Additional polypeptides with apparent molecular weights of 21,000, 23,000, and 92,000 become major components by 22 h after infection. Concomitantly, some peptides in the 45,000-75,000 mol wt range become less prominent. In synchronized cells the relative staining capacity of the six bands in the 45,000-75,000 mol wt range changes during the cell cycle. Synthesis of at least some matrix polypeptides occures in all phases of the cell cycle, although there is decreased synthesis in late S/G2. In the absence of protein synthesis after cell division, at least some polypeptides in the 45,000-75,000 mol wt range survive nuclear dispersal and subsequent reformation during mitosis. The possible significance of this subnuclear structure with regard to structure-function relationships within the nucleus during virus replication and during the life cycle of the cell is discussed.

Uremic Neurotoxin in the Middle Molecular Weight Range

1980 ◽  
Vol 4 (2) ◽  
pp. 116-120 ◽  
Author(s):  
N.K. Man ◽  
G. Cueille ◽  
J. Zingraff ◽  
J. Boudet ◽  
A. Sausse ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Molecular Weight Range ◽  
Weight Range

Aqueous polypropylene glycol induces swelling and severe plasticization of high Tg amphiphilic copolymers containing hexafluoroisopropanol groups

Soft Matter ◽  
2020 ◽  
Vol 16 (27) ◽  
pp. 6362-6370
Author(s):  
Siyuan Li ◽  
Bryan D. Vogt
Keyword(s):  
Molecular Weight ◽  
Propylene Glycol ◽  
Polypropylene Glycol ◽  
Amphiphilic Copolymers ◽  
Molecular Weight Range ◽  
Weight Range ◽  
Poly Propylene

Not too big, not too small, but a narrow molecular weight range for poly(propylene glycol) where swelling of the copolymer increases tremendously for poly(propylene glycol).

scholarly journals Experimental and Theoretical Studies of Carboxylic Polymers with Low Molecular Weight as Inhibitors for Calcium Carbonate Scale

Crystals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 406 ◽  
Author(s):  
Yuwei Zuo ◽  
Wenzhong Yang ◽  
Kegui Zhang ◽  
Yun Chen ◽  
Xiaoshuang Yin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Calcium Carbonate ◽  
Normal Growth ◽  
Dynamics Simulation ◽  
Low Molecular Weight ◽  
X Ray Diffraction ◽  
Crystal Surfaces ◽  
Molecular Weight Range ◽  
Scale Inhibition ◽  
Diffraction Patterns

Poly acrylic acid (PAA) and polyepoxysuccinic acid (PESA) were investigated as scale inhibitors. The static experiments certified that PAA was superior to PESA for the inhibition of calcium carbonate in the low molecular weight range. The X-ray diffraction patterns suggest that the effect of PAA on the calcite (1 0 4) and (1 1 0) crystal plane was more obvious. Scanning electron microscopy was used to study the surface morphology of the depositions, which indicated that the addition of scale inhibitors could disturb the normal growth of CaCO3 scale. The transmittance ratio of ferric oxide demonstrated that PAA had a better dispersion performance than PESA. The molecular dynamics simulation and quantum calculation were selected to theoretically explore the mechanism and structure of scale inhibitors, indicating that the interaction of PAA with (1 0 4) and (1 1 0) calcite crystal surfaces was stronger than PESA. In addition, the results indicated that the PAA with negative charge more easily adsorbed free Ca2+ in the aqueous phase. Based on these observations, PAA exhibited better scale inhibition and dispersion effects than PESA in the case of low molecular weight.

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