Cyclic voltammetric studies of 1,2,4-trihydroxy-9,10-anthraquinone, its interaction with calf thymus DNA and anti-leukemic activity on MOLT-4 cell lines: a comparison with anthracycline anticancer drugs

2011 ◽  
Vol 24 (9) ◽  
pp. 774-785 ◽  
Author(s):  
Piyal Das ◽  
Partha Sarathi Guin ◽  
Parikshit C. Mandal ◽  
Mausumi Paul ◽  
Santanu Paul ◽  
...  
Keyword(s):  
Cell Lines ◽  
Anticancer Drugs ◽  
Calf Thymus Dna ◽  
Cyclic Voltammetric ◽  
Calf Thymus ◽  
Voltammetric Studies ◽  
Cyclic Voltammetric Studies

Spectroscopic investigation of interaction of 6-methoxyflavanone and its β-cyclodextrin inclusion complex with calf thymus DNA

2012 ◽  
Vol 66 (8) ◽  
Author(s):  
Sameena Yousuf ◽  
Israel Enoch
Keyword(s):  
Fluorescence Intensity ◽  
Molecular Modelling ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Calf Thymus ◽  
Hyperchromic Effect ◽  
Voltammetric Studies ◽  
Presence And Absence ◽  
Different Characteristics ◽  
Cyclic Voltammetric Studies

AbstractThe interaction of 6-methoxyflavanone (6MF, 6-methoxy-2-phenyl-4H-1-benzopyran-4-one) with calf thymus DNA (ctDNA) was investigated by absorption spectroscopy, fluorescence spectroscopy, and cyclic voltammetry in the presence and absence of β-cyclodextrin (β-CD) acting as capping agent. Molecular modelling was used to optimise the study of 6MF-β-CD and 6MF-DNA interactions. Enhancement in the fluorescence intensity of 6MF was observed due to the formation of 1 : 1 complex with β-CD. In the presence and absence of DNA, 6MF showed different characteristics such as hyperchromic effect, red shift of absorption spectra and fluorescence quenching of 6MF due to binding between 6MF and ctDNA. The nature of the binding group was found to be different for the 6MF-ctDNA and 6MF-ctDNA-β-CD systems. An increase in fluorescence intensity was observed for the 6MF-ctDNA system while varying the concentration of β-CD due to encapsulation of a part of 6MF in cyclodextrin. The results are compatible with the possibility of the interaction of dihydrobenzopyran-4-one moiety of 6MF with ctDNA as well as with β-CD. Cyclic voltammetric studies confirmed the binding interaction between 6MF and ctDNA in the absence and presence of β-CD and molecular modelling explains the site of the interaction of 6MF with cyclodextrin and ctDNA.

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