Kinetic study on the prooxidative effect of vitamin C on the autoxidation of glycerol trioleate in micelles

2006 ◽  
Vol 19 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Zai-Qun Liu
2020 ◽  
Vol 11 (1) ◽  
pp. 8374-8388

In this study, bisubstrate kinetics mechanism models were correlated with the experimental data of ascorbyl oleate using the new lipase preparation, Candida antarctica NS 88011, and kinetic parameters (Vmax, Km, and Ki) were estimated. The highest conversion (49.42 %) was observed at 1 h of reaction using the ascorbic acid/oleic acid molar ratio of 1:9, 70 ºC and 30 % of enzymatic loading. The Ping Pong Bi-Bi model showed better congruence with the experimental data for all the effects evaluated. The kinetic constants showed that the lipase had an identical affinity, with a value of 0.81 for both substrates and inhibitory constant (Ki) of ascorbic acid (1.959) much higher than oleic acid (0.0008). It has been demonstrated that lipase has good operational stability (14th cycle). The results obtained with the new immobilized enzyme are valuable to elucidate the reaction mechanism. In addition, it represents an important contribution to optimize the reaction and create strategies to increase the productivity of the esters of vitamin C.


1989 ◽  
Vol 991 (2) ◽  
pp. 276-279 ◽  
Author(s):  
Kazuo Mukai ◽  
Motoi Nishimura ◽  
Kazuhiko Ishizu ◽  
Yoichi Kitamura
Keyword(s):  

2008 ◽  
Vol 56 (18) ◽  
pp. 8387-8393 ◽  
Author(s):  
Isabel Odriozola-Serrano ◽  
Robert Soliva-Fortuny ◽  
Vicente Gimeno-Añó ◽  
Olga Martín-Belloso

1981 ◽  
Vol 46 (2) ◽  
pp. 636-637 ◽  
Author(s):  
M. A. RAO ◽  
C. Y. LEE ◽  
J. KATZ ◽  
H. J. COOLEY

2008 ◽  
Vol 56 (12) ◽  
pp. 4406-4417 ◽  
Author(s):  
Shuji Mitani ◽  
Aya Ouchi ◽  
Emi Watanabe ◽  
Yu Kanesaki ◽  
Shin-Ichi Nagaoka ◽  
...  

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