New Approaches for Absolute Quantification of Stable‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag

Karsten Schnatbaum; Victor Solis‐Mezarino; Daniil Pokrovsky; Frederike Schäfer; Dennis Nagl; Lars Hornberger; Johannes Zerweck; Tobias Knaute; Julia Avramova‐Nehmer; Mike Schutkowski; Veit Hornung; Holger Wenschuh; Moritz Carl Völker‐Albert; Axel Imhof; Ulf Reimer

doi:10.1002/pmic.202000007

New Approaches for Absolute Quantification of Stable‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag

PROTEOMICS ◽

10.1002/pmic.202000007 ◽

2020 ◽

Vol 20 (10) ◽

pp. 2000007

Author(s):

Karsten Schnatbaum ◽

Victor Solis‐Mezarino ◽

Daniil Pokrovsky ◽

Frederike Schäfer ◽

Dennis Nagl ◽

...

Keyword(s):

Stable Isotope ◽

Absolute Quantification ◽

Targeted Proteomics ◽

New Approaches

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Front Cover: New Approaches for Absolute Quantification of Stable‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag

PROTEOMICS ◽

10.1002/pmic.202070081 ◽

2020 ◽

Vol 20 (10) ◽

pp. 2070081

Author(s):

Karsten Schnatbaum ◽

Victor Solis‐Mezarino ◽

Daniil Pokrovsky ◽

Frederike Schäfer ◽

Dennis Nagl ◽

...

Keyword(s):

Stable Isotope ◽

Absolute Quantification ◽

Targeted Proteomics ◽

Front Cover ◽

New Approaches

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Peptide Biosynthesis with Stable Isotope Labeling from a Cell-free Expression System for Targeted Proteomics with Absolute Quantification

Molecular & Cellular Proteomics ◽

10.1074/mcp.o115.056507 ◽

2016 ◽

Vol 15 (8) ◽

pp. 2819-2828 ◽

Cited By ~ 7

Author(s):

Feng Xian ◽

Jin Zi ◽

Quanhui Wang ◽

Xiaomin Lou ◽

Haidan Sun ◽

...

Keyword(s):

Stable Isotope ◽

Isotope Labeling ◽

Expression System ◽

Absolute Quantification ◽

Stable Isotope Labeling ◽

Free Expression ◽

Targeted Proteomics ◽

A Cell ◽

Peptide Biosynthesis ◽

Cell Free Expression System

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Absolute quantification of protein and post-translational modification abundance with stable isotope–labeled synthetic peptides

Nature Protocols ◽

10.1038/nprot.2010.196 ◽

2011 ◽

Vol 6 (2) ◽

pp. 175-186 ◽

Cited By ~ 108

Author(s):

Arminja N Kettenbach ◽

John Rush ◽

Scott A Gerber

Keyword(s):

Stable Isotope ◽

Synthetic Peptides ◽

Absolute Quantification ◽

Post Translational Modification

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Abstract 2203: A SRM/MRM based targeted proteomics strategy for absolute quantification of potential biomarkers of TKI sensitivity in EGFR mutated lung adenocarcinoma

10.1158/1538-7445.am2017-2203 ◽

2017 ◽

Author(s):

Shivangi Awasthi ◽

Xu Zhang ◽

Tapan Maity ◽

Benjamin L. Oyler ◽

David R. Goodlett ◽

...

Keyword(s):

Lung Adenocarcinoma ◽

Absolute Quantification ◽

Targeted Proteomics ◽

Egfr Mutated ◽

Potential Biomarkers

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Absolute Quantification of Apolipoproteins Following Treatment with Omega-3 Carboxylic Acids and Fenofibrate Using a High Precision Stable Isotope-labeled Recombinant Protein Fragments Based SRM Assay

Molecular & Cellular Proteomics ◽

10.1074/mcp.ra119.001765 ◽

2019 ◽

Vol 18 (12) ◽

pp. 2433-2446 ◽

Cited By ~ 3

Author(s):

Andreas Hober ◽

Fredrik Edfors ◽

Maria Ryaboshapkina ◽

Jonas Malmqvist ◽

Louise Rosengren ◽

...

Keyword(s):

Stable Isotope ◽

Recombinant Protein ◽

Carboxylic Acids ◽

High Precision ◽

Absolute Quantification ◽

Omega 3 ◽

Protein Fragments

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Multiple-Enzyme-Digestion Strategy Improves Accuracy and Sensitivity of Label- and Standard-Free Absolute Quantification to a Level That Is Achievable by Analysis with Stable Isotope-Labeled Standard Spiking

Journal of Proteome Research ◽

10.1021/acs.jproteome.8b00549 ◽

2018 ◽

Cited By ~ 2

Author(s):

Jacek R Wiśniewski ◽

Christine Wegler ◽

Per Artursson

Keyword(s):

Stable Isotope ◽

Absolute Quantification ◽

Enzyme Digestion

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Absolute quantification of protein NP24 in tomato fruit by liquid chromatography/tandem mass spectrometry using stable isotope-labelled tryptic peptide standard

Food Chemistry ◽

10.1016/j.foodchem.2014.10.008 ◽

2015 ◽

Vol 173 ◽

pp. 238-242 ◽

Cited By ~ 8

Author(s):

Katsunari Ippoushi ◽

Motoe Sasanuma ◽

Hideaki Oike ◽

Masuko Kobori ◽

Mari Maeda-Yamamoto

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Stable Isotope ◽

Tandem Mass Spectrometry ◽

Tryptic Peptide ◽

Tomato Fruit ◽

Absolute Quantification ◽

Tandem Mass ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

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Absolute Quantification of Phosphorylation on the Kinase Activation Loop of Cellular Focal Adhesion Kinase by Stable Isotope Dilution Liquid Chromatography/Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac900204f ◽

2009 ◽

Vol 81 (9) ◽

pp. 3304-3313 ◽

Cited By ~ 46

Author(s):

Eugene Ciccimaro ◽

Steven K. Hanks ◽

Kenneth H. Yu ◽

Ian A. Blair

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Stable Isotope ◽

Focal Adhesion ◽

Isotope Dilution ◽

Absolute Quantification ◽

Activation Loop ◽

Liquid Chromatography Mass Spectrometry ◽

Kinase Activation ◽

Stable Isotope Dilution

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Absolute Quantification of N-Glycosylation of Alpha-Fetoprotein Using Parallel Reaction Monitoring with Stable Isotope-Labeled N-Glycopeptide as an Internal Standard

Analytical Chemistry ◽

10.1021/acs.analchem.0c02563 ◽

2020 ◽

Vol 92 (18) ◽

pp. 12588-12595

Author(s):

Kwang Hoe Kim ◽

Sang Yoon Lee ◽

Dong Geun Kim ◽

Soo-Youn Lee ◽

Jin Young Kim ◽

...

Keyword(s):

Stable Isotope ◽

Internal Standard ◽

Absolute Quantification ◽

Alpha Fetoprotein ◽

Reaction Monitoring ◽

Parallel Reaction ◽

Parallel Reaction Monitoring

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ICP-MS for absolute quantification of proteins for heteroatom-tagged, targeted proteomics

TrAC Trends in Analytical Chemistry ◽

10.1016/j.trac.2012.07.020 ◽

2012 ◽

Vol 40 ◽

pp. 52-63 ◽

Cited By ~ 66

Author(s):

Alfredo Sanz-Medel ◽

María Montes-Bayón ◽

Jörg Bettmer ◽

M. Luisa Fernández-Sanchez ◽

Jorge Ruiz Encinar

Keyword(s):

Absolute Quantification ◽

Targeted Proteomics ◽

Icp Ms

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