scholarly journals Comparative Proteomic Profiling of Methicillin‐Susceptible and Resistant Staphylococcus aureus

PROTEOMICS ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 1900221
Author(s):  
Zhen Xu ◽  
Jiazhen Chen ◽  
Kostas Vougas ◽  
Ajit Shah ◽  
Haroun Shah ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Proteomic Profiling

scholarly journals iTRAQ-Based Quantitative Proteomic Profiling of Staphylococcus aureus Under Different Osmotic Stress Conditions

2019 ◽  
Vol 10 ◽  
Author(s):  
Tinghong Ming ◽  
Lingxin Geng ◽  
Ying Feng ◽  
Chenyang Lu ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Osmotic Stress ◽  
Stress Conditions ◽  
Proteomic Profiling

scholarly journals Proteomic profiling of the endogenous peptides of MRSA and MSSA

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12508
Author(s):  
Haixia Tu ◽  
Fei Xu ◽  
Yiwei Cheng ◽  
Qianglong Pan ◽  
Xiao Cai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Metabolic Regulation ◽  
Proteomic Profiling ◽  
Positive Bacterium ◽  
Future Studies ◽  
Endogenous Peptides ◽  
C Terminus ◽  
Severe Infections ◽  
Functional Regulation

Staphylococcus aureus is a Gram-positive bacterium that can cause diverse skin and soft tissue infections. Methicillin-resistant Staphylococcus aureus (MRSA) can cause more severe infections than methicillin-susceptible Staphylococcus aureus (MSSA). Nevertheless, the physiological and metabolic regulation of MSSA and MRSA has not been well studied. In light of the increased interest in endogenous peptides and recognition of the important roles that they play, we studied the endogenous peptidome of MSSA and MRSA. We identified 1,065 endogenous peptides, among which 435 were differentially expressed (DE), with 292 MSSA-abundant endogenous peptides and 35 MRSA-abundant endogenous peptides. MSSA-abundant endogenous peptides have significantly enriched “VXXXK” motif of at the C-terminus. MSSA-abundant endogenous peptides are involved in penicillin-binding and immune responses, whereas MRSA-abundant endogenous peptides are associated with antibiotic resistance and increased toxicity. Our characterization of the peptidome of MSSA and MRSA provides a rich resource for future studies to explore the functional regulation of drug resistance in S. aureus and may also help elucidate the mechanisms of its pathogenicity and the development of treatments.

Activated ADI pathway: the initiator of intermediate vancomycin resistance inStaphylococcus aureus

2017 ◽  
Vol 63 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Xin-Ee Tan ◽  
Hui-min Neoh ◽  
Mee-Lee Looi ◽  
Siok Fong Chin ◽  
Longzhu Cui ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Cell Walls ◽  
Vancomycin Resistance ◽  
Cell Wall Biosynthesis ◽  
Ornithine Carbamoyltransferase ◽  
Proteomic Profiling ◽  
Arginine Catabolism ◽  
By Products ◽  
Catabolism Pathway

Comparative proteomic profiling between 2 vancomycin-intermediate Staphylococcus aureus (VISA) strains, Mu50Ω-vraSm and Mu50Ω-vraSm-graRm, and vancomycin-susceptible S. aureus (VSSA) strain Mu50Ω revealed upregulated levels of catabolic ornithine carbamoyltransferase (ArcB) of the arginine catabolism pathway in VISA strains. Subsequent analyses showed that the VISA strains have higher levels of cellular ATP and ammonia, which are by-products of arginine catabolism, and displayed thicker cell walls. We postulate that elevated cytoplasmic ammonia and ATP molecules, resulting from activated arginine catabolism upon acquisition of vraS and graR mutations, are important requirements facilitating cell wall biosynthesis, thereby contributing to thickened cell wall and consequently reduced vancomycin susceptibility in VISA strains.

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