Decreased E-Cadherin in MCF7 Human Breast Cancer Cells Forming Multicellular Spheroids Exposed to Simulated Microgravity

PROTEOMICS ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1800015 ◽  
Author(s):  
Jayashree Sahana ◽  
Mohamed Zakaria Nassef ◽  
Markus Wehland ◽  
Sascha Kopp ◽  
Marcus Krüger ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Simulated Microgravity ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Multicellular Spheroids ◽  
E Cadherin

scholarly journals Short-term effects of simulated microgravity on morphology and gene expression in human breast cancer cells

2019 ◽  
Vol 106 (4) ◽  
pp. 311-322 ◽  
Author(s):  
F Strube ◽  
M Infanger ◽  
C Dietz ◽  
A Romswinkel ◽  
A Kraus
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Simulated Microgravity ◽  
Adherent Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Adhesion Properties

Introduction Microgravity has been shown to impose various effects on breast cancer cells. We exposed human breast cancer cells to simulated microgravity and studied morphology and alterations in gene expression. Materials and methods Human breast cancer cells were exposed to simulated microgravity in a random positioning machine (RPM) for 24 h. Morphology was observed under light microscopy, and gene alteration was studied by qPCR. Results After 24 h, formation of three-dimensional structures (spheroids) occurred. BRCA1 expression was significantly increased (1.9×, p < 0.05) in the adherent cells under simulated microgravity compared to the control. Expression of KRAS was significantly decreased (0.6×, p < 0.05) in the adherent cells compared to the control. VCAM1 was significantly upregulated (6.6×, 2.0×, p < 0.05 each) in the adherent cells under simulated microgravity and in the spheroids. VIM expression was significantly downregulated (0.45×, 0.44×, p < 0.05 each) in the adherent cells under simulated microgravity and in the spheroids. There was no significant alteration in the expression of MAPK1, MMP13, PTEN, and TP53. Conclusions Simulated microgravity induces spheroid formation in human breast cancer cells within 24 h and alters gene expression toward modified adhesion properties, enhanced cell repair, and phenotype preservation. Further insights into the underlying mechanisms could open up the way toward new therapies.

E-cadherin decreased human breast cancer cells sensitivity to staurosporine by up-regulating Bcl-2 expression

2009 ◽  
Vol 481 (1) ◽  
pp. 116-122 ◽  
Author(s):  
Liying Wang ◽  
Zengxia Li ◽  
Can Wang ◽  
Yong Yang ◽  
Lidong Sun ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
E Cadherin
Sign in / Sign up

Export Citation Format

Share Document