Front Cover: Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes

PROTEOMICS ◽  
2017 ◽  
Vol 17 (19) ◽  
pp. 1770141 ◽  
Author(s):  
Nicole A. Haverland ◽  
Matthew Waas ◽  
Ioanna Ntai ◽  
Theodore Keppel ◽  
Rebekah L. Gundry ◽  
...  
Keyword(s):  
Cell Surface ◽  
Human Lymphocytes ◽  
Front Cover ◽  
Cover Cell

Surface morphology and agglutination of lectin-treated human lymphocytes and lymphoblasts

1976 ◽  
Vol 21 (3) ◽  
pp. 563-578
Author(s):  
J.H. Temmink ◽  
J.G. Collard ◽  
J. Roosien ◽  
J.F. Van den Bosch
Keyword(s):  
Cell Surface ◽  
Surface Morphology ◽  
Human Lymphocytes ◽  
Cell Type ◽  
A Cell ◽  
Human Peripheral Lymphocytes ◽  
Normal Human ◽  
Altered Cell ◽  
Induced Changes ◽  
Cell Surface Morphology

Two human lymphoblasts (Raji and EB3) and normal human peripheral lymphocytes were exposed to different concentrations of Concanavalin A and wheat germ agglutinin. The lectin-induced agglutination was determined and correlated with lectin-induced changes in the surface morphology of these cells as studied in a scanning electron microscope. Whenever the lectin induced high agglutinability in a cell type, it also invariably had a smoothing effect on the cell surface. In contrast, when cells did not agglutinate well with a certain lectin, their cell surface remained essentially rough (villous) after addition of the lectin. The correlation found between increased agglutinability and altered cell surface morphology upon treatment with certain lectins suggests that both phenomena result from one and the same process. Additional evidence for this postulate is presented.

scholarly journals β-blockers Reverse Agonist-Induced β2-AR Downregulation Regardless of Their Signaling Profile

2020 ◽  
Vol 21 (2) ◽  
pp. 512
Author(s):  
Sonia Maccari ◽  
Vanessa Vezzi ◽  
Federica Barbagallo ◽  
Tonino Stati ◽  
Barbara Ascione ◽  
...  
Keyword(s):  
Cell Surface ◽  
Human Lymphocytes ◽  
Cell Types ◽  
Hek293 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mrna Levels ◽  
Receptor Density ◽  
Biased Agonism ◽  
Β Blocker ◽  
Β Blockers

Altered β-adrenergic receptor (β-AR) density has been reported in cells, animals, and humans receiving β-blocker treatment. In some cases, β-AR density is upregulated, but in others, it is unaffected or even reduced. Collectively, these results would imply that changes in β-AR density and β-blockade are not related. However, it has still not been clarified whether the effects of β-blockers on receptor density are related to their ability to activate different β-AR signaling pathways. To this aim, five clinically relevant β-blockers endowed with inverse, partial or biased agonism at the β2-AR were evaluated for their effects on β2-AR density in both human embryonic kidney 293 (HEK293) cells expressing exogenous FLAG-tagged human β2-ARs and human lymphocytes expressing endogenous β2-ARs. Cell surface β2-AR density was measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Treatment with propranolol, carvedilol, pindolol, sotalol, or timolol did not induce any significant change in surface β2-AR density in both HEK293 cells and human lymphocytes. On the contrary, treatment with the β-AR agonist isoproterenol reduced the number of cell surface β2-ARs in the tested cell types without affecting β2-AR-mRNA levels. Isoproterenol-induced effects on receptor density were completely antagonized by β-blocker treatment. In conclusion, the agonistic activity of β-blockers does not exert an important effect on short-term regulation of β2-AR density.

scholarly journals T cell receptor-dependent activation of human lymphocytes through cell surface ganglioside GT1b: implications for innate immunity

2000 ◽  
Vol 30 (11) ◽  
pp. 3199-3206 ◽  
Author(s):  
Jack F. Bukowski ◽  
Maria G. Roncarolo ◽  
Hergen Spits ◽  
Michael S. Krangel ◽  
Craig T. Morita ◽  
...  
Keyword(s):  
Innate Immunity ◽  
T Cell ◽  
Cell Surface ◽  
T Cell Receptor ◽  
Human Lymphocytes ◽  
Cell Receptor ◽  
Ganglioside Gt1b

Accessibility of cell surface thiols in human lymphocytes is altered by ionophores or OKT-3 antibody

1986 ◽  
Vol 140 (3) ◽  
pp. 999-1006 ◽  
Author(s):  
Margit Balázs ◽  
János Matkó ◽  
János Szöllösi ◽  
László Mátyus ◽  
Mack J. Fulwyler ◽  
...  
Keyword(s):  
Cell Surface ◽  
Human Lymphocytes

scholarly journals Binding, internalization, and hydrolysis of low density lipoprotein in long-term lymphoid cell lines from a normal subject and a patient with homozygous familial hypercholesterolemia.

1976 ◽  
Vol 144 (2) ◽  
pp. 444-455 ◽  
Author(s):  
Y K Ho ◽  
M S Brown ◽  
H J Kayden ◽  
J L Goldstein
Keyword(s):  
Cell Surface ◽  
Familial Hypercholesterolemia ◽  
Human Lymphocytes ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lymphoid Cells ◽  
Low Density ◽  
High Affinity ◽  
Hydrolysis Of

Long-term established human lymphoid cells were shown to possess high affinity cell surface receptors for low density lipoprotein (LDL), the major cholesterol-carrying protein in human plasma. Binding of LDL to these receptors was followed by internalization of the lipoprotein and hydrolysis of its protein and cholesteryl ester components. Cultured lymphocytes from a patient with the homozygous form of familial hypercholesterolemia lacked cell surface LDL receptors and therefore failed to take up and degrade the lipoprotein with high affinity. Cultured human lymphocytes should prove useful for further studies of: (a) the relation between cholesterol metabolism and cellular function and (b) the mechanism by which LDL binding at the cell surface leads to internalization of the lipoprotein.

Human lymphocytes: similarity of B and T cell surface morphology

Science ◽  
1975 ◽  
Vol 188 (4189) ◽  
pp. 732-734 ◽  
Author(s):  
E. Alexander ◽  
B Wetzel
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Surface Morphology ◽  
Human Lymphocytes ◽  
Cell Surface Morphology

Direct visualization of binding, aggregation and internalization of human growth hormone in cultured human lymphocytes

1984 ◽  
Vol 107 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Rina Eshet ◽  
Shulamit Peleg ◽  
Zvi Laron
Keyword(s):  
Growth Hormone ◽  
Cell Surface ◽  
Human Growth Hormone ◽  
Direct Observation ◽  
Hormone Receptor ◽  
Human Lymphocytes ◽  
Human Growth ◽  
Direct Visualization ◽  
Down Regulation ◽  
Receptor Complexes

Abstract. The distribution of human growth hormone (hGH) receptor complexes on IM-9 cultured lymphocytes was studied using fluorescein and 125I-labelled hGH (F-hGH and [125I]hGH). The cells labelled with F-hGH were visualized with a sensitive video intensification microscopic system which permitted direct observation of the location of the fluorescent hormone on the surface of the living lymphocytes. At 4°C F-hGH bound diffusely to the cell surface and remained dispersed but following incubation for 30 min at 37°C the hormone receptor complexes aggregated into patches on the cell surface and formed a single cap on one pole of the cell. Progressive internalization into the cell was demonstrated at 37°C with [125I]hGH. It is hypothesized that the aggregation and internalization of the hGH receptor complexes are associated with the action and degradation of the hormone and probably also with the mechanism of down-regulation of the receptors.

scholarly journals Inside Front Cover: Cell Wall Proteome of Sugarcane Young and Mature Leaves and Stems

PROTEOMICS ◽  
2018 ◽  
Vol 18 (2) ◽  
pp. 18700025
Author(s):  
Juliana G. Fonseca ◽  
Maria J. Calderan-Rodrigues ◽  
Fabrício E. de Moraes ◽  
Thaís R. Cataldi ◽  
Elisabeth Jamet ◽  
...  
Keyword(s):  
Cell Wall ◽  
Front Cover ◽  
Mature Leaves ◽  
Cell Wall Proteome ◽  
Cover Cell

scholarly journals Occurrence of cytochrome b558 in B-cell lineage of human lymphocytes

Blood ◽  
1990 ◽  
Vol 75 (2) ◽  
pp. 458-461
Author(s):  
S Kobayashi ◽  
S Imajoh-Ohmi ◽  
M Nakamura ◽  
S Kanegasaki
Keyword(s):  
B Cells ◽  
Cell Surface ◽  
B Cell ◽  
B Lymphocytes ◽  
Plasma Cells ◽  
Human Lymphocytes ◽  
Cell Lineage ◽  
Superoxide Generation ◽  
Cell Stage ◽  
Cytochrome B558

Cytochrome b558, involved in superoxide generation in phagocytes, was found to be expressed on the cell surface of most normal peripheral B lymphocytes. The cytochrome was not found on the surface of peripheral T lymphocytes, natural killer cells, or peripheral lymphocytes derived from patients with X-linked chronic granulomatous disease. On stimulation, at least half of peripheral B lymphocytes could generate superoxide anion as detected by superoxide dismutase-sensitive nitroblue tetrazorium reduction. Cytochrome b558 was not present on the surface of pre-pre B cells or pre-B cells, but did appear at the early B-cell stage. It disappeared from the B-cell surface during terminal differentiation to plasma cells. The transient expression of the cytochrome in B-cell lineage may indicate that superoxide generation is important for the function of these cells at certain stages.

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