Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata

PROTEOMICS ◽  
2017 ◽  
Vol 17 (21) ◽  
pp. 1700165 ◽  
Author(s):  
Yifan Zhai ◽  
Xiaobin Fan ◽  
Zhenjuan Yin ◽  
Xiangzhao Yue ◽  
Xingyuan Men ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Chitin Synthase ◽  
Mythimna Separata ◽  
Oriental Armyworm

Molecular cloning, characterization and functional analysis of GluCl from the oriental armyworm, Mythimna separata Walker

2019 ◽  
Vol 156 ◽  
pp. 56-62 ◽  
Author(s):  
Jin-da Wang ◽  
Li-fei Chen ◽  
Dong-jiang Lin ◽  
Jia-song Zhang ◽  
Ji-han Zhao ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Cloning ◽  
Mythimna Separata ◽  
Oriental Armyworm

scholarly journals Functional Analysis of the Chemosensory Protein MsepCSP8 From the Oriental Armyworm Mythimna separata

2018 ◽  
Vol 9 ◽  
Author(s):  
Aneela Younas ◽  
Muhammad I. Waris ◽  
Muhammad Tahir ul Qamar ◽  
Muhammad Shaaban ◽  
Sean M. Prager ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Mythimna Separata ◽  
Chemosensory Protein ◽  
Oriental Armyworm

Expression and functional analysis of cytochrome P450 genes in the integument of the oriental armyworm, Mythimna separata ( Walker )

2020 ◽  
Vol 77 (1) ◽  
pp. 577-587
Author(s):  
Yong Huang ◽  
Hongqin Yin ◽  
Zeng Zhu ◽  
Xingchuan Jiang ◽  
Xiuxia Li ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Cytochrome P450 ◽  
Mythimna Separata ◽  
Cytochrome P450 Genes ◽  
Oriental Armyworm

scholarly journals Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker)

2019 ◽  
Vol 10 ◽  
Author(s):  
Kunpeng Zhang ◽  
Yilu Feng ◽  
Lixiao Du ◽  
Shanshan Gao ◽  
Hang Yan ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Mythimna Separata ◽  
Oriental Armyworm

Transcriptional identification of differentially expressed genes during the prepupal–pupal transition in the oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae)

2021 ◽  
pp. 1-14
Author(s):  
Peirong Li ◽  
Xinru Li ◽  
Wei Wang ◽  
Xiaoling Tan ◽  
Xiaoqi Wang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Metabolic Pathways ◽  
Developmental Stages ◽  
Kegg Pathway ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Mythimna Separata ◽  
Kegg Pathway Analysis ◽  
Oriental Armyworm ◽  
Lepidoptera Noctuidae

Abstract The oriental armyworm, Mythimna separata (Walker) is a serious pest of agriculture that does particular damage to Gramineae crops in Asia, Europe, and Oceania. Metamorphosis is a key developmental stage in insects, although the genes underlying the metamorphic transition in M. separata remain largely unknown. Here, we sequenced the transcriptomes of five stages; mature larvae (ML), wandering (W), and pupation (1, 5, and 10 days after pupation, designated P1, P5, and P10) to identify transition-associated genes. Four libraries were generated, with 22,884, 23,534, 26,643, and 33,238 differentially expressed genes (DEGs) for the ML-vs-W, W-vs-P1, P1-vs-P5, and P5-vs-P10, respectively. Gene ontology enrichment analysis of DEGs showed that genes regulating the biosynthesis of the membrane and integral components of the membrane, which includes the cuticular protein (CP), 20-hydroxyecdysone (20E), and juvenile hormone (JH) biosynthesis, were enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that DEGs were enriched in the metabolic pathways. Of these DEGs, thirty CP, seventeen 20E, and seven JH genes were differentially expressed across the developmental stages. For transcriptome validation, ten CP, 20E, and JH-related genes were selected and verified by real-time PCR quantitative. Collectively, our results provided a basis for further studies of the molecular mechanism of metamorphosis in M. separata.

scholarly journals Identification of 35 C-Type Lectins in the Oriental Armyworm, Mythimna separata (Walker)

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 559
Author(s):  
Hao Li ◽  
Fang-Fang Liu ◽  
Li-Qing Fu ◽  
Ze Liu ◽  
Wen-Ting Zhang ◽  
...  
Keyword(s):  
Fat Body ◽  
Early Stage ◽  
Average Length ◽  
Phylogenetic Analyses ◽  
Endocrine System ◽  
Economic Loss ◽  
Mythimna Separata ◽  
Oriental Armyworm ◽  
Potential Ligand ◽  
Cellular Immune

Insect C-type lectins (CTLs) play vital roles in modulating humoral and cellular immune responses. The oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae) is a migratory pest that causes significant economic loss in agriculture. CTLs have not yet been systematically identified in M. separata. In this study, we first constructed a transcriptome of M. separata larvae, generating a total of 45,888 unigenes with an average length of 910 bp. Unigenes were functionally annotated in six databases: NR, GO, KEGG, Pfam, eggNOG, and Swiss-Prot. Unigenes were enriched in functional pathways, such as those of signal transduction, endocrine system, cellular community, and immune system. Thirty-five unigenes encoding C-type lectins were identified, including CTL-S1~CTL-S6 (single CRD) and IML-1~IML-29 (dual CRD). Phylogenetic analyses showed dramatic lineage-specific expansions of IMLs. Sequence alignment and structural modeling identified potential ligand-interacting residues. Real-time qPCR revealed that CTL-Ss mainly express in eggs and early stage larvae, while IMLs mainly express in mid-late-stage larvae, pupae, and adults. In naïve larvae, hemocytes, fat body, and epidermis are the major tissues that express CTLs. In larvae challenged by Escherichia coli, Staphylococcus aureus, or Beauveria bassiana, the expression of different CTLs was stimulated in hemocytes, fat body and midgut. The present study will help further explore functions of M. separata CTLs.

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