scholarly journals Inside Front Cover: Multi-mode acquisition (MMA): An MS/MS acquisition strategy for maximizing selectivity, specificity and sensitivity of DIA product ion spectra

PROTEOMICS ◽  
2016 ◽  
Vol 16 (15-16) ◽  
pp. NA-NA
Author(s):  
Brad J. Williams ◽  
Steve J. Ciavarini ◽  
Curt Devlin ◽  
Steven M. Cohn ◽  
Rong Xie ◽  
...  
Keyword(s):  
Front Cover ◽  
Specificity And Sensitivity ◽  
Acquisition Strategy ◽  
Multi Mode ◽  
Product Ion Spectra

Multi-mode acquisition (MMA): An MS/MS acquisition strategy for maximizing selectivity, specificity and sensitivity of DIA product ion spectra

PROTEOMICS ◽  
2016 ◽  
Vol 16 (15-16) ◽  
pp. 2284-2301 ◽  
Author(s):  
Brad J. Williams ◽  
Steve J. Ciavarini ◽  
Curt Devlin ◽  
Steven M. Cohn ◽  
Rong Xie ◽  
...  
Keyword(s):  
Specificity And Sensitivity ◽  
Acquisition Strategy ◽  
Multi Mode ◽  
Product Ion Spectra

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

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