Updating the proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs

PROTEOMICS ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 609-613 ◽  
Author(s):  
Stefanie Dietz ◽  
Christian Lassek ◽  
Sarah-Lena Mack ◽  
Mathias Ritzmann ◽  
Julia Stadler ◽  
...  
Keyword(s):  
Mycoplasma Suis ◽  
Hemotrophic Mycoplasma

scholarly journals Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma

2013 ◽  
Vol 44 (1) ◽  
pp. 6 ◽  
Author(s):  
Albina Sokoli ◽  
Katrin Groebel ◽  
Katharina Hoelzle ◽  
Werner M Amselgruber ◽  
José M Mateos ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Damage ◽  
Cell Tropism ◽  
Endothelial Cell Damage ◽  
Mycoplasma Suis ◽  
Hemotrophic Mycoplasma ◽  
Insight Into

scholarly journals Insights into the Gene Expression Profile of Uncultivable Hemotrophic Mycoplasma suis during Acute Infection, Obtained Using Proteome Analysis

2012 ◽  
Vol 194 (6) ◽  
pp. 1505-1514 ◽  
Author(s):  
K. M. Felder ◽  
P. M. Carranza ◽  
P. M. Gehrig ◽  
B. Roschitzki ◽  
S. Barkow-Oesterreicher ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Acute Infection ◽  
Proteome Analysis ◽  
Mycoplasma Suis ◽  
Hemotrophic Mycoplasma

scholarly journals Complete Genome Sequence of the Hemotrophic Mycoplasma suis Strain KI3806

2011 ◽  
Vol 193 (9) ◽  
pp. 2369-2370 ◽  
Author(s):  
J. Oehlerking ◽  
M. Kube ◽  
K. M. Felder ◽  
D. Matter ◽  
M. M. Wittenbrink ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mycoplasma Suis ◽  
Hemotrophic Mycoplasma

scholarly journals Mycoplasma suis Invades Porcine Erythrocytes

2008 ◽  
Vol 77 (2) ◽  
pp. 576-584 ◽  
Author(s):  
K. Groebel ◽  
K. Hoelzle ◽  
M. M. Wittenbrink ◽  
U. Ziegler ◽  
L. E. Hoelzle
Keyword(s):  
Laser Scanning ◽  
Fluorescent Labeling ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Cells ◽  
Mycoplasma Suis ◽  
Transmission Electron ◽  
Blood Smears ◽  
Hemotrophic Mycoplasma ◽  
First Time

ABSTRACT Mycoplasma suis belongs to the hemotrophic mycoplasma group and causes infectious anemia in pigs. According to the present state of knowledge, this organism adheres to the surface of erythrocytes but does not invade them. We found a novel M. suis isolate that caused severe anemia in pigs with a fatal disease course. Interestingly, only marginal numbers of the bacteria were visible on and between the erythrocytes in acridine orange-stained blood smears for acutely diseased pigs, whereas very high loads of M. suis were detected in the same blood samples by quantitative PCR. These findings indicated that M. suis is capable of invading erythrocytes. By use of fluorescent labeling of M. suis and examination by confocal laser scanning microscopy, as well as scanning and transmission electron microscopy, we proved that the localization of M. suis was intracellular. This organism invades erythrocytes in an endocytosis-like process and is initially surrounded by two membranes, and it was also found floating freely in the cytoplasm. In conclusion, we were able to prove for the first time that a member of the hemotrophic mycoplasma group is able to invade the erythrocytes of its host. Such colonization should protect the bacterial cells from the host's immune response and hamper antibiotic treatment. In addition, an intracellular life cycle may explain the chronic nature of hemotrophic mycoplasma infections and should serve as the foundation for novel strategies in hemotrophic mycoplasma research (e.g., treatment or prophylaxis).

Exploratory study of Mycoplasma suis (Eperythrozoon suis) on four commercial pig farms in southern Brazil

2007 ◽  
Vol 160 (2) ◽  
pp. 50-53 ◽  
Author(s):  
A. M. S. Guimaraes ◽  
A. W. Biondo ◽  
A. C. Lara ◽  
J. B. Messick
Keyword(s):  
Exploratory Study ◽  
Southern Brazil ◽  
Mycoplasma Suis ◽  
Pig Farms

A reply to “The 1.8kb DNA fragment formerly confirmed as Mycoplasma suis (M. suis) specific was originated from the porcine genome”

2009 ◽  
Vol 138 (1-2) ◽  
pp. 199
Author(s):  
Ludwig E. Hoelzle ◽  
Dagmar Adelt ◽  
Katharina Hoelzle ◽  
Karl Heinritzi ◽  
Max M. Wittenbrink
Keyword(s):  
Porcine Genome ◽  
Mycoplasma Suis ◽  
Dna Fragment

A quantitative TaqMan PCR assay for the detection of Mycoplasma suis

2011 ◽  
Vol 111 (2) ◽  
pp. 417-425 ◽  
Author(s):  
A.M.S. Guimaraes ◽  
R.F.C. Vieira ◽  
R. Poletto ◽  
R. Vemulapalli ◽  
A.P. Santos ◽  
...  
Keyword(s):  
Pcr Assay ◽  
Mycoplasma Suis ◽  
Taqman Pcr

scholarly journals Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari)

2011 ◽  
Vol 20 (1) ◽  
pp. 75-77 ◽  
Author(s):  
Rafael Felipe da Costa Vieira ◽  
Marcelo Beltrão Molento ◽  
Ana Marcia Sá Guimarães ◽  
Andrea Pires dos Santos ◽  
Marcelo Bonat ◽  
...  
Keyword(s):  
Animal Health ◽  
Surveillance Program ◽  
Mycoplasma Suis ◽  
16S Rna ◽  
Blood Smears ◽  
Blood Smear Examination ◽  
Polymerase Chain ◽  
Tayassu Tajacu ◽  
Dna Pcr ◽  
Active Surveillance Program

Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.

scholarly journals Use of Recombinant Antigens To Detect Antibodies against Mycoplasma suis, with Correlation of Serological Results to Hematological Findings

2007 ◽  
Vol 14 (12) ◽  
pp. 1616-1622 ◽  
Author(s):  
Katharina Hoelzle ◽  
Julia Grimm ◽  
Mathias Ritzmann ◽  
Karl Heinritzi ◽  
Paul Torgerson ◽  
...  
Keyword(s):  
Large Scale ◽  
Animal Experiments ◽  
Antigen Specificity ◽  
Predictive Values ◽  
Whole Cell ◽  
Worldwide Distribution ◽  
Mycoplasma Suis ◽  
Field Samples ◽  
Weaning Piglets ◽  
Cell Elisa

ABSTRACT Porcine eperythrozoonosis is a disease with worldwide distribution caused by the unculturable hemotrophic bacterium Mycoplasma suis. Current serological testing utilizes crude M. suis antigens purified from the blood of experimentally infected pigs. These antigens show high variability and are restricted to specialized laboratories. We evaluated a novel serological assay based on two recombinant M. suis antigens (rMSG1 and rHspA1). Antigen specificity was proven by means of sera raised against nonhemotrophic mycoplasma and other relevant bacteria. Using experimental and convalescent-phase sera, rMSG1 and rHspA1 enzyme-linked immunosorbent assays (ELISAs) demonstrated sensitivities, specificities, and predictive values (94.0 to 100.0%) equal to or higher than those of the M. suis whole-cell ELISA. Field samples from 120 weaning piglets grouped by quantitative PCR results were used to evaluate the diagnostic capability of the new ELISA systems in comparison to that of the whole-cell ELISA. Assuming a 100.0% specificity of the PCR, the whole-cell ELISA, rHspA1 ELISA, and rMSG1 ELISA showed specificities of 84.8%, 83.8%, and 90.6% and sensitivities of 61.5%, 74.0% and 58.1%, respectively. Cohen's kappa coefficients comparing the recombinant ELISAs to the whole-cell ELISA indicate moderate to substantial agreement. The detection of anti-MSG1 and/or anti-HspA1 antibodies in pigs was significantly correlated with decreased hematocrit, erythrocyte numbers, and hemoglobin concentrations, indicating that a single seropositive result is connected with clinical and etiological significance. In conclusion, rMSG1 and rHspA1 are sensitive and specific serological and infection markers which are for the first time used independently of animal experiments. They are especially fit to be used in routine diagnosis, pathogenesis studies, and large-scale epidemiological investigations.

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