Effects of Fe deficiency on the protein profile of Brassica napus phloem sap

PROTEOMICS ◽  
2015 ◽  
Vol 15 (22) ◽  
pp. 3835-3853 ◽  
Author(s):  
Elain Gutierrez-Carbonell ◽  
Giuseppe Lattanzio ◽  
Alfonso Albacete ◽  
Juan José Rios ◽  
Julia Kehr ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Protein Profile ◽  
Fe Deficiency ◽  
Phloem Sap

scholarly journals Protein profile of Lupinus texensis phloem sap exudates: Searching for Fe- and Zn-containing proteins

PROTEOMICS ◽  
2013 ◽  
Vol 13 (15) ◽  
pp. 2283-2296 ◽  
Author(s):  
Giuseppe Lattanzio ◽  
Sofía Andaluz ◽  
Andrea Matros ◽  
Juan José Calvete ◽  
Julia Kehr ◽  
...  
Keyword(s):  
Protein Profile ◽  
Phloem Sap

scholarly journals Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply

2015 ◽  
Vol 6 ◽  
Author(s):  
Laura Ceballos-Laita ◽  
Elain Gutierrez-Carbonell ◽  
Giuseppe Lattanzio ◽  
Saul Vázquez ◽  
Bruno Contreras-Moreira ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Protein Profile ◽  
Fe Deficiency ◽  
Apoplastic Fluid

Changes in expression of proteins involved in alleviation of Fe-deficiency by sulfur nutrition in Brassica napus L.

2013 ◽  
Vol 35 (10) ◽  
pp. 3037-3045 ◽  
Author(s):  
Sowbiya Muneer ◽  
Bok Rye Lee ◽  
Dong Won Bae ◽  
Tae Hwan Kim
Keyword(s):  
Brassica Napus ◽  
Fe Deficiency ◽  
Brassica Napus L ◽  
Sulfur Nutrition

scholarly journals Label-Free Quantitative Proteomics Analysis in Susceptible and Resistant Brassica napus Cultivars Infected with Xanthomonas campestris pv. campestris

2021 ◽  
Vol 9 (2) ◽  
pp. 253
Author(s):  
Md Tabibul Islam ◽  
Bok-Rye Lee ◽  
Van Hien La ◽  
Dong-Won Bae ◽  
Woo-Jin Jung ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Brassica Napus ◽  
Quantitative Proteomics ◽  
Xanthomonas Campestris ◽  
Rna Binding ◽  
Protein Profile ◽  
Mitochondrial Outer Membrane ◽  
Ps Ii ◽  
Related Proteins ◽  
Xanthomonas Campestris Pv Campestris

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is the main disease of cruciferous vegetables. To characterize the resistance mechanism in the Brassica napus–Xcc pathosystem, Xcc-responsive proteins in susceptible (cv. Mosa) and resistant (cv. Capitol) cultivars were investigated using gel-free quantitative proteomics and analysis of gene expression. This allowed us to identify 158 and 163 differentially expressed proteins following Xcc infection in cv. Mosa and cv. Capitol, respectively, and to classify them into five major categories including antioxidative systems, proteolysis, photosynthesis, redox, and innate immunity. All proteins involved in protein degradation such as the protease complex, proteasome subunits, and ATP-dependent Clp protease proteolytic subunits, were upregulated only in cv. Mosa, in which higher hydrogen peroxide accumulation concurred with upregulated superoxide dismutase. In cv. Capitol, photosystem II (PS II)-related proteins were downregulated (excepting PS II 22 kDa), whereas the PS I proteins, ATP synthase, and ferredoxin-NADP+ reductase, were upregulated. For redox-related proteins, upregulation of thioredoxin, 2-cys peroxiredoxin, and glutathione S-transferase occurred in cv. Capitol, consistent with higher NADH-, ascorbate-, and glutathione-based reducing potential, whereas the proteins involved in the C2 oxidative cycle and glycolysis were highly activated in cv. Mosa. Most innate immunity-related proteins, including zinc finger domain (ZFD)-containing protein, glycine-rich RNA-binding protein (GRP) and mitochondrial outer membrane porin, were highly enhanced in cv. Capitol, concomitant with enhanced expression of ZFD and GRP genes. Distinguishable differences in the protein profile between the two cultivars deserves higher importance for breeding programs and understanding of disease resistance in the B. napus–Xcc pathosystem.

scholarly journals Supraoptimal Iron Nutrition of Brassica napus Plants Suppresses the Iron Uptake of Chloroplasts by Down-Regulating Chloroplast Ferric Chelate Reductase

2021 ◽  
Vol 12 ◽  
Author(s):  
Máté Sági-Kazár ◽  
Helga Zelenyánszki ◽  
Brigitta Müller ◽  
Barnabás Cseh ◽  
Balázs Gyuris ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Photosynthetic Apparatus ◽  
Iron Nutrition ◽  
Fe Deficiency ◽  
Chloroplast Envelope ◽  
Substrate Affinity ◽  
Mesophyll Cells ◽  
Ferric Chelate Reductase ◽  
Fe Uptake ◽  
Fe Nutrition

Iron (Fe) is an essential micronutrient for plants. Due to the requirement for Fe of the photosynthetic apparatus, the majority of shoot Fe content is localised in the chloroplasts of mesophyll cells. The reduction-based mechanism has prime importance in the Fe uptake of chloroplasts operated by Ferric Reductase Oxidase 7 (FRO7) in the inner chloroplast envelope membrane. Orthologue of Arabidopsis thaliana FRO7 was identified in the Brassica napus genome. GFP-tagged construct of BnFRO7 showed integration to the chloroplast. The time-scale expression pattern of BnFRO7 was studied under three different conditions: deficient, optimal, and supraoptimal Fe nutrition in both leaves developed before and during the treatments. Although Fe deficiency has not increased BnFRO7 expression, the slight overload in the Fe nutrition of the plants induced significant alterations in both the pattern and extent of its expression leading to the transcript level suppression. The Fe uptake of isolated chloroplasts decreased under both Fe deficiency and supraoptimal Fe nutrition. Since the enzymatic characteristics of the ferric chelate reductase (FCR) activity of purified chloroplast inner envelope membranes showed a significant loss for the substrate affinity with an unchanged saturation rate, protein level regulation mechanisms are suggested to be also involved in the suppression of the reduction-based Fe uptake of chloroplasts together with the saturation of the requirement for Fe.

Changes Induced by Fe Deficiency and Fe Resupply in the Root Protein Profile of a Peach-Almond Hybrid Rootstock

2013 ◽  
Vol 12 (3) ◽  
pp. 1162-1172 ◽  
Author(s):  
Jorge Rodríguez-Celma ◽  
Giuseppe Lattanzio ◽  
Sergio Jiménez ◽  
Jean-Francois Briat ◽  
Javier Abadía ◽  
...  
Keyword(s):  
Protein Profile ◽  
Fe Deficiency
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