Functional characterization of epithelial ovarian cancer histotypes by drug target based protein signaling activation mapping: Implications for personalized cancer therapy

PROTEOMICS ◽  
2015 ◽  
Vol 15 (2-3) ◽  
pp. 365-373 ◽  
Author(s):  
Maria Isabella Sereni ◽  
Elisa Baldelli ◽  
Guido Gambara ◽  
Jianghong Deng ◽  
Laura Zanotti ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Therapy ◽  
Drug Target ◽  
Functional Characterization ◽  
Protein Signaling ◽  
Signaling Activation ◽  
Personalized Cancer Therapy ◽  
Personalized Cancer ◽  
Activation Mapping

Personalized cancer therapy for ovarian cancer patients.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e16521-e16521
Author(s):  
Alfredo Aguilar ◽  
Priscila I. Valdiviezo ◽  
Carolina Ibañez ◽  
Maria L Bravo ◽  
Pamela Gonzales ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Therapy ◽  
Cancer Patients ◽  
Personalized Cancer Therapy ◽  
Personalized Cancer

Abstract 1187: Personalized cancer therapy for ovarian cancer patients.

2013 ◽  
Author(s):  
Maria L. Bravo ◽  
Pamela Gonzalez ◽  
Sumie Kato ◽  
Marcelo Garrido ◽  
Jorge Brañes ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Therapy ◽  
Cancer Patients ◽  
Personalized Cancer Therapy ◽  
Personalized Cancer

scholarly journals 3D Bioprinted cancer models: Revolutionizing personalized cancer therapy

2021 ◽  
Vol 14 (4) ◽  
pp. 101015
Author(s):  
Robin Augustine ◽  
Sumama Nuthana Kalva ◽  
Rashid Ahmad ◽  
Alap Ali Zahid ◽  
Shajia Hasan ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Cancer Models ◽  
Personalized Cancer Therapy ◽  
Personalized Cancer

scholarly journals Functional Characterization of the Oxantel-Sensitive Acetylcholine Receptor from Trichuris muris

2021 ◽  
Vol 14 (7) ◽  
pp. 698
Author(s):  
Tina V. A. Hansen ◽  
Richard K. Grencis ◽  
Mohamed Issouf ◽  
Cédric Neveu ◽  
Claude L. Charvet
Keyword(s):  
Single Dose ◽  
Mouse Model ◽  
Xenopus Laevis ◽  
Acetylcholine Receptor ◽  
Drug Target ◽  
Functional Characterization ◽  
Xenopus Laevis Oocytes ◽  
Trichuris Muris ◽  
Electrode Voltage

The human whipworm, Trichuris trichiura, is estimated to infect 289.6 million people globally. Control of human trichuriasis is a particular challenge, as most anthelmintics have a limited single-dose efficacy, with the striking exception of the narrow-spectrum anthelmintic, oxantel. We recently identified a novel ACR-16-like subunit from the pig whipworm, T. suis which gave rise to a functional acetylcholine receptor (nAChR) preferentially activated by oxantel. However, there is no ion channel described in the mouse model parasite T. muris so far. Here, we have identified the ACR-16-like and ACR-19 subunits from T. muris, and performed the functional characterization of the receptors in Xenopus laevis oocytes using two-electrode voltage-clamp electrophysiology. We found that the ACR-16-like subunit from T. muris formed a homomeric receptor gated by acetylcholine whereas the ACR-19 failed to create a functional channel. The subsequent pharmacological analysis of the Tmu-ACR-16-like receptor revealed that acetylcholine and oxantel were equally potent. The Tmu-ACR-16-like was more responsive to the toxic agonist epibatidine, but insensitive to pyrantel, in contrast to the Tsu-ACR-16-like receptor. These findings confirm that the ACR-16-like nAChR from Trichuris spp. is a preferential drug target for oxantel, and highlights the pharmacological difference between Trichuris species.

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