Metaproteomics: Evaluation of protein extraction from activated sludge

PROTEOMICS ◽  
2014 ◽  
Vol 14 (21-22) ◽  
pp. 2535-2539 ◽  
Author(s):  
Susan Hove Hansen ◽  
Allan Stensballe ◽  
Per Halkjaer Nielsen ◽  
Florian-Alexander Herbst
Keyword(s):  
Activated Sludge ◽  
Protein Extraction

Protein extraction from activated sludge

2006 ◽  
Vol 54 (1) ◽  
pp. 175-181 ◽  
Author(s):  
M. Denecke
Keyword(s):  
Activated Sludge ◽  
Crude Extract ◽  
Extracellular Polymeric Substances ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Bacterial Cells ◽  
Uronic Acids ◽  
Isolated Cells ◽  
Humic Compounds ◽  
Immunoblot Technique

Two methods for the separation of protein originating from activated sludge were compared. In one method, the total protein was isolated out of the activated sludge (crude extract). These samples included all dissolved proteins originating from the bacterial cells and biofilm made up of extracellular polymeric substances (EPS). Every time polyacrylamide gel electrophoresis (PAGE) was done, the protein bands from samples of crude extract were covered by polymeric substances including carbohydrates, uronic acids or humic compounds. Using the immunoblot technique it was possible to demonstrate the presence of the heat shock protein HSP70 in crude extracts of activated sludge. The comparison of protein fingerprints required that clear and distinct bands appear on the PAGE analysis. To this end, a procedure to separates bacterial cells from the EPS was developed. Bacterial cells were separated by incubation with EDTA and subsequent filtration. The isolated cells were directly incubated in a sample buffer.

Experimental Research on Protein Extraction from Excess Activated Sludge by Papain Hydrolysis

2012 ◽  
Vol 518-523 ◽  
pp. 3367-3370 ◽  
Author(s):  
Wen Feng Zhang ◽  
Rui Jing Su ◽  
Guang Lin Guo ◽  
Deng Xin Li
Keyword(s):  
Heavy Metals ◽  
Amino Acid ◽  
Activated Sludge ◽  
Protein Extraction ◽  
Animal Feed ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Feed Additives ◽  
Hydrolysis Time

The excess activated sludge from the Shanghai Qingpu sewage treatment plant was hydrolyzed by papain hydrolysis to extract protein. The results showed that the conditions of optimal papain hydrolysis process are described as follows: enzyme/substrate 6%, pH 6.5, the ratio of liquid to solid 4:1, hydrolysis time 5.5h and hydrolysis temperature 55 °C. On this basis, the contents of heavy metals and some kinds of amino acid from the extracts, while security and nutrition were taken into consideration, were determined. The results showed that the contents of heavy metals were lower, living up to the Health Standard for Animal Feed (China), and some kinds of amino acid were essential for animal growth in the extracts. So it was possible to take the extracts as animal feed additives.

Comparison of protein extraction methods from excess activated sludge

Chemosphere ◽  
2020 ◽  
Vol 249 ◽  
pp. 126107 ◽  
Author(s):  
Jianlei Gao ◽  
Wei Weng ◽  
Yixin Yan ◽  
Yingchun Wang ◽  
Qikun Wang
Keyword(s):  
Activated Sludge ◽  
Protein Extraction ◽  
Extraction Methods

Protein extraction from activated sludge: An analytical approach

2008 ◽  
Vol 42 (8-9) ◽  
pp. 1867-1878 ◽  
Author(s):  
M. Ras ◽  
E. Girbal-Neuhauser ◽  
E. Paul ◽  
M. Spérandio ◽  
D. Lefebvre
Keyword(s):  
Activated Sludge ◽  
Analytical Approach ◽  
Protein Extraction

Reduction of Excess Activated Sludge by Controlling the Motion of Ferrite Particles

2014 ◽  
Vol 134 (4) ◽  
pp. 475-476
Author(s):  
Mahmudul Kabir ◽  
Tatsuya Chiba ◽  
Masafumi Suzuki ◽  
Noboru Yoshimura
Keyword(s):  
Activated Sludge
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