Application of multiplexed cysteine-labeled complex protein sample for 2D electrophoretic gel alignment

PROTEOMICS ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 1777-1780 ◽  
Author(s):  
Perttu Haimi ◽  
Sidona Sikorskaite-Gudziuniene ◽  
Danas Baniulis
Keyword(s):  
Protein Sample ◽  
Complex Protein

2D-DIGE A Powerful Tool for Proteome Analysis

2020 ◽  
pp. 67-73
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Great Sensitivity ◽  
Two Dimensional Gel Electrophoresis ◽  
2D Dige ◽  
Protein Sample ◽  
Different Types ◽  
Complex Protein ◽  
2D Gel

In the recent past, two dimensional gel electrophoresis has emerged as a powerful molecular biology tool for the comparative expression profiling of complex protein sample. It involves the separation as well as the resolution of diverse proteins sample on the basis of isoelectric points and molecular mass of protein in two dimension ways. In this way, it reflects the view of overall proteome status including differentiation in protein expression levels, post-translational modifications etc. Moreover, this allows the identification of novel biological signatures, which may give a particular identity of pathological background to cells or tissues associated with various types of cancers and neurological disorders. Therefore, by utilizing such tools, one can clearly investigate and compare the effects of particular drugs on cells of tissues and also one can analyze the effects of disease on the basis of variations in protein expression profile at broad spectrum. Recently, to get more error-less and accurate proteome profile, conventional 2-D gel electrophoresis has been enhanced with the inclusion of different types of protein labeling dyes which enables a more comparative analysis of diverse protein sample in a single 2-D gel. In this advanced technique (2-D-DIGE), protein samples are labeled with three different types of CyDyes (Cy2, Cy3, and Cy5) separately and combined and further resolved on the same gel. This will facilitate the more accurate spot matching on a single gel platform and will also minimize the experimental variations as commonly reported in the conventional 2D-gel electrophoresis. Therefore, in the present proteomic research era, 2D-DIGE has proved to be an extremely powerful tool with great sensitivity for up to 125 ng of proteins in clinical research volubility especially, neurological and cancer related disorders.

Low-abundant protein extraction from complex protein sample using a novel continuous aqueous two-phase systems device

2012 ◽  
Vol 36 (2) ◽  
pp. 391-399 ◽  
Author(s):  
Patricia Vázquez-Villegas ◽  
Edith Espitia-Saloma ◽  
Marco Rito-Palomares ◽  
Oscar Aguilar
Keyword(s):  
Protein Extraction ◽  
Two Phase ◽  
Abundant Protein ◽  
Protein Sample ◽  
Complex Protein ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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