Facile synthesis of magnetic poly(styrene-co-4-vinylbenzene-boronic acid) microspheres for selective enrichment of glycopeptides

PROTEOMICS ◽  
2015 ◽  
Vol 15 (13) ◽  
pp. 2158-2165 ◽  
Author(s):  
Mengyi Wang ◽  
Xiangmin Zhang ◽  
Chunhui Deng
2017 ◽  
Vol 41 (22) ◽  
pp. 13399-13407 ◽  
Author(s):  
Lei Gu ◽  
Yun Wang ◽  
Juan Han ◽  
Lei Wang ◽  
Xu Tang ◽  
...  

We developed an efficient and mild method for the preparation of boronic acid-functionalized magnetic nanoparticles (MNPs), and the selective separation of fructose from a sample solution was demonstrated for the first time.


2016 ◽  
Vol 30 ◽  
pp. 190-195 ◽  
Author(s):  
Peipei Song ◽  
Peiwu Huang ◽  
Tengjun Huang ◽  
Hua Li ◽  
Wendong Chen ◽  
...  

2020 ◽  
Vol 43 (7) ◽  
pp. 1348-1355 ◽  
Author(s):  
Muhammad Salman Sajid ◽  
Fahmida Jabeen ◽  
Dilshad Hussain ◽  
Qurra‐tul‐Ann Afza Gardner ◽  
Muhammad Naeem Ashiq ◽  
...  

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Changfen Bi ◽  
Ye Yuan ◽  
Yuran Tu ◽  
Jiahui Wu ◽  
Yulu Liang ◽  
...  

AbstractThe development of methods to effectively capture N-glycopeptides from the complex biological samples is crucial to N-glycoproteome profiling. Herein, the hydrophilic chitosan–functionalized magnetic graphene nanocomposites (denoted as Fe3O4-GO@PDA-Chitosan) were designed and synthesized via a simple two-step modification (dopamine self-polymerization and Michael addition). The Fe3O4-GO@PDA-Chitosan nanocomposites exhibited good performances with low detection limit (0.4 fmol·μL−1), good selectivity (mixture of bovine serum albumin and horseradish peroxidase tryptic digests at a molar ration of 10:1), good repeatability (4 times), high binding capacity (75 mg·g−1). Moreover, Fe3O4-GO@PDA-Chitosan nanocomposites were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 393 N-linked glycopeptides, representing 195 different glycoproteins and 458 glycosylation sites were identified. This study provides a feasible strategy for the surface functionalized novel materials for isolation and enrichment of N-glycopeptides.


The Analyst ◽  
2017 ◽  
Vol 142 (23) ◽  
pp. 4494-4502 ◽  
Author(s):  
Daojin Li ◽  
Zijun Bie

Boronate affinity materials, as efficient sorbents for extraction, separation and enrichment of glycoproteins, have attracted more and more attention in recent years.


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