Proteomic changes in the brain of the western painted turtle (Chrysemys picta bellii ) during exposure to anoxia

PROTEOMICS ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 1587-1597 ◽  
Author(s):  
Richard W. Smith ◽  
Phil Cash ◽  
David W. Hogg ◽  
Leslie T. Buck
Keyword(s):  
Chrysemys Picta ◽  
Painted Turtle ◽  
Chrysemys Picta Bellii ◽  
The Brain

Response of protein synthesis to anoxia and recovery in anoxia-tolerant hepatocytes

1993 ◽  
Vol 265 (1) ◽  
pp. R41-R48 ◽  
Author(s):  
S. C. Land ◽  
L. T. Buck ◽  
P. W. Hochachka
Keyword(s):  
Protein Synthesis ◽  
Chrysemys Picta ◽  
Painted Turtle ◽  
Urea Synthesis ◽  
O2 Consumption ◽  
Purine Nucleotide ◽  
Turnover Rates ◽  
Atp Turnover ◽  
Chrysemys Picta Bellii ◽  
Metabolic Suppression

Hepatocytes from the western painted turtle (Chrysemys picta bellii) display a profound metabolic suppression under anoxia. Fractional rates of protein synthesis fell by 92% during 12 h anoxia at 25 degrees C and were indistinguishable from the rate obtained with cycloheximide. Normoxic recovery saw protein synthesis increase to 160% of control values and return to normal after 2 h. The GTP-to-GDP ratio, implicated in the control of translation, fell threefold during anoxia. Purine nucleotide phosphate profiles suggest that this change occurs through increasing concentrations of ADP and GDP, with concentrations of ATP and GTP and total purines remaining constant. The normoxic cost for protein synthesis was calculated at 47.6 +/- 6.8 mmol ATP/g protein. Normoxic protein synthesis accounted for 36% of overall ATP turnover rates, close to the extent of O2 consumption inhibitable by cycloheximide (28%). Under anoxia, the proportion of ATP turnover utilized by protein synthesis did not change significantly. ATP turnover rates for urea synthesis reflected a similar pattern, falling 72% under anoxia. These results reflect the cell's ability to suppress protein synthesis under anoxia in a manner that is coordinated with the reduction in total metabolic rate.

scholarly journals Skeletal Muscle Histidine Containing Dipeptide Contents are Increased in Freshwater Turtles (Chrysemys picta bellii) with Cold-Acclimation

2021 ◽  
Author(s):  
Eimear Dolan ◽  
Daniel E Warren ◽  
Roger C Harris ◽  
Craig Sale ◽  
Bruno Gualano ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Temperature Sensitivity ◽  
Ph Regulation ◽  
Chrysemys Picta ◽  
Painted Turtle ◽  
Freshwater Turtles ◽  
Acid Base ◽  
Cold Temperatures ◽  
Chrysemys Picta Bellii ◽  
Buffering Power

Freshwater turtles found in higher latitudes can experience extreme challenges to acid-base homeostasis while overwintering, due to a combination of cold temperatures along with the potential for environmental hypoxia. Histidine containing dipeptides (HCDs; carnosine, anserine and balenine) may facilitate pH regulation in response to these challenges, through their role as pH buffers. We measured the HCDs content of three tissues (liver, cardiac muscle and skeletal muscle) from the anoxia-tolerant painted turtle (Chrysemys picta bellii) acclimated to either 3 or 20 C. HCDs were detected in all tissues, with the highest content shown in the skeletal muscle. Turtles acclimated to 3 C had more HCD in their skeletal muscle than those acclimated to 20 C (carnosine = 20.8 +/- 4.5 vs 12.5 +/- 5.9 mmol/kg DM; ES = 1.59 (95%CI: 0.16 - 3.00), P = 0.013). The higher HCD content observed in the skeletal muscle of the cold-acclimated turtles suggests a role in acid-base regulation in response to physiological challenges associated with living in the cold, with the increase possibly related to the temperature sensitivity of carnosine's dissociation constant and buffering power of the skeletal muscle during anoxic submergence.

First report on the non-native species, Western painted turtle (Chrysemys picta bellii), in the wild, Republic of Korea

2020 ◽  
Vol 38 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Il-Kook Park ◽  
Kyungwon Lee ◽  
Ji-Hyun Jeong ◽  
Hyun-Been Lee ◽  
Kyo Soung Koo
Keyword(s):  
Native Species ◽  
Chrysemys Picta ◽  
Republic Of Korea ◽  
Painted Turtle ◽  
First Report ◽  
Chrysemys Picta Bellii ◽  
In The Wild
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