Analysis of proteome and frost tolerance in chromosome 5A and 5B reciprocal substitution lines between two winter wheats during long-term cold acclimation

PROTEOMICS ◽  
2011 ◽  
Vol 12 (1) ◽  
pp. 68-85 ◽  
Author(s):  
Pavel Vítámvás ◽  
Ilja T. Prášil ◽  
Klára Kosová ◽  
Sébastien Planchon ◽  
Jenny Renaut
Keyword(s):  
Cold Acclimation ◽  
Frost Tolerance ◽  
Substitution Lines ◽  
Chromosome 5A

scholarly journals Different Accumulation of Free Amino Acids during Short- and Long-Term Osmotic Stress in Wheat

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Zita Kovács ◽  
Livia Simon-Sarkadi ◽  
Ildikó Vashegyi ◽  
Gábor Kocsy
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Osmotic Stress ◽  
Free Amino Acid ◽  
Free Amino ◽  
Substitution Line ◽  
Substitution Lines ◽  
Chromosome 5A ◽  
Short And Long Term

The effect of wheat chromosome 5A on free amino acid accumulation induced by osmotic stress was compared in chromosome 5A substitution lines with different freezing tolerance. Treatment with 15% polyethylene glycol (PEG) resulted in greater total free amino acid content even after 3 days compared to the controls. The ratio of amino acids belonging to various amino acid families differed after 3-week treatment in the control and PEG-treated plants only in the case of the freezing-sensitive substitution line. There was a transient increase with a maximum after 3 days in the amounts of several amino acids, after which their concentrations exhibited a more gradual increase. During the first days of osmotic stress the Glu, Gln, Asp, Asn, Thr, Ser, Leu, and His concentrations were greater in the tolerant substitution line than in the sensitive one, while the opposite relationship was observed at the end of the PEG treatment. The coordinated changes in the levels of individual amino acids indicated that they are involved in both the short- and long-term responses to osmotic stress. The alterations differed in the two chromosome 5A substitution lines, depending on the stress tolerance of the chromosome donor genotype.

Skeletal and cardiac muscle protein turnover during cold acclimation in young rats

2000 ◽  
Vol 278 (3) ◽  
pp. R705-R711 ◽  
Author(s):  
T. A. McAllister ◽  
J. R. Thompson ◽  
S. E. Samuels
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Cardiac Muscle ◽  
Cold Acclimation ◽  
Protein Turnover ◽  
Muscle Protein ◽  
Muscle Protein Turnover ◽  
Protein Mass ◽  
The Absolute

The effect of long-term cold exposure on skeletal and cardiac muscle protein turnover was investigated in young growing animals. Two groups of 36 male 28-day-old rats were maintained at either 5°C (cold) or 25°C (control). Rates of protein synthesis and degradation were measured in vivo on days 5, 10, 15, and 20. Protein mass by day 20 was ∼28% lower in skeletal muscle (gastrocnemius and soleus) and ∼24% higher in heart in cold compared with control rats ( P < 0.05). In skeletal muscle, the fractional rates of protein synthesis ( k syn) and degradation ( k deg) were not significantly different between cold and control rats, although k syn was lower (approximately −26%) in cold rats on day 5; consequent to the lower protein mass, the absolute rates of protein synthesis (approximately −21%; P < 0.05) and degradation (approximately −13%; P < 0.1) were lower in cold compared with control rats. In heart, overall, k syn(approximately +12%; P < 0.1) and k deg(approximately +22%; P < 0.05) were higher in cold compared with control rats; consequently, the absolute rates of synthesis (approximately +44%) and degradation (approximately +54%) were higher in cold compared with control rats ( P < 0.05). Plasma triiodothyronine concentration was higher ( P < 0.05) in cold compared with control rats. These data indicate that long-term cold acclimation in skeletal muscle is associated with the establishment of a new homeostasis in protein turnover with decreased protein mass and normal fractional rates of protein turnover. In heart, unlike skeletal muscle, rates of protein turnover did not appear to immediately return to normal as increased rates of protein turnover were observed beyond day 5. These data also indicate that increased rates of protein turnover in skeletal muscle are unlikely to contribute to increased metabolic heat production during cold acclimation.

scholarly journals COR/LEA Proteins as Indicators of Frost Tolerance in Triticeae: A Comparison of Controlled versus Field Conditions

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 789
Author(s):  
Klára Kosová ◽  
Miroslav Klíma ◽  
Ilja Tom Prášil ◽  
Pavel Vítámvás
Keyword(s):  
Cold Acclimation ◽  
Field Experiments ◽  
Quantitative Relationship ◽  
Field Trials ◽  
Frost Tolerance ◽  
Late Embryogenesis Abundant ◽  
Field Conditions ◽  
Lea Proteins ◽  
Winter Cereals ◽  
Vernalisation Requirement

Low temperatures in the autumn induce enhanced expression/relative accumulation of several cold-inducible transcripts/proteins with protective functions from Late-embryogenesis-abundant (LEA) superfamily including dehydrins. Several studies dealing with plants grown under controlled conditions revealed a correlation (significant quantitative relationship) between dehydrin transcript/protein relative accumulation and plant frost tolerance. However, to apply these results in breeding, field experiments are necessary. The aim of the review is to provide a summary of the studies dealing with the relationships between plant acquired frost tolerance and COR/LEA transcripts/proteins relative accumulation in cereals grown in controlled and field conditions. The impacts of cold acclimation and vernalisation processes on the ability of winter-type Triticeae to accumulate COR/LEA proteins are discussed. The factors determining dehydrin relative accumulation under controlled cold acclimation treatments versus field trials during winter seasons are discussed. In conclusion, it can be stated that dehydrins could be used as suitable indicators of winter survival in field-grown winter cereals but only in plant prior to the fulfilment of vernalisation requirement.

scholarly journals Pleiotropic effect of chromosome 5A and the mvp mutation on the metabolite profile during cold acclimation and the vegetative/generative transition in wheat

2015 ◽  
Vol 15 (1) ◽  
pp. 57 ◽  
Author(s):  
Zsófia Juhász ◽  
Ákos Boldizsár ◽  
Tibor Nagy ◽  
Gábor Kocsy ◽  
Ferenc Marincs ◽  
...  
Keyword(s):  
Cold Acclimation ◽  
Metabolite Profile ◽  
Pleiotropic Effect ◽  
Chromosome 5A

Frost tolerance in winter wheat cultivars: different effects of chromosome 5A and association with microsatellite alleles

2013 ◽  
Vol 57 (1) ◽  
pp. 184-188 ◽  
Author(s):  
G. Ganeva ◽  
T. Petrova ◽  
S. Landjeva ◽  
E. Todorovska ◽  
S. Kolev ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Frost Tolerance ◽  
Wheat Cultivars ◽  
Winter Wheat Cultivars ◽  
Chromosome 5A ◽  
Microsatellite Alleles

FROST TOLERANCE INDUCED BY COLD ACCLIMATION IN OLIVE (OLEA EUROPAEA) L.

2002 ◽  
pp. 473-477 ◽  
Author(s):  
F. Bartolozzi ◽  
F. Cerquaglia ◽  
L. Coppari ◽  
G. Fontanazza
Keyword(s):  
Cold Acclimation ◽  
Olea Europaea ◽  
Frost Tolerance ◽  
Olea Europaea L ◽  
Olea Europaéa

scholarly journals Dynamic modelling of cold-hardiness in tea buds by imitating past temperature memory

2020 ◽  
Author(s):  
Kensuke Kimura ◽  
Daisuke Yasutake ◽  
Takahiro Oki ◽  
Koichiro Yoshida ◽  
Masaharu Kitano
Keyword(s):  
Cold Stress ◽  
Cold Acclimation ◽  
Dynamic Model ◽  
Cold Hardiness ◽  
Global Climate ◽  
Effective Means ◽  
Dynamic Modelling ◽  
Short Term ◽  
Mean Square Errors

Abstract Background and Aims Most perennial plants memorize cold stress for a certain period and retrieve the memories for cold acclimation and deacclimation, which leads to seasonal changes in cold-hardiness. Therefore, a model for evaluating cold stress memories is required for predicting cold-hardiness and for future frost risk assessments under warming climates. In this study we develop a new dynamic model of cold-hardiness by introducing a function imitating past temperature memory in the processes of cold acclimation and deacclimation. Methods We formulated the past temperature memory for plants using thermal time weighted by a forgetting function, and thereby proposed a dynamic model of cold-hardiness. We used the buds of tea plants (Camellia sinensis) from two cultivars, ‘Yabukita’ and ‘Yutakamidori’, to calibrate and validate this model based on 10 years of observed cold-hardiness data. Key Results The model captured more than 90 % of the observed variation in cold-hardiness and predicted accurate values for both cultivars, with root mean square errors of ~1.0 °C. The optimized forgetting function indicated that the tea buds memorized both short-term (recent days) and long-term (previous months) temperatures. The memories can drive short-term processes such as increasing/decreasing the content of carbohydrates, proteins and antioxidants in the buds, as well as long-term processes such as determining the bud phenological stage, both of which vary with cold-hardiness. Conclusions The use of a forgetting function is an effective means of understanding temperature memories in plants and will aid in developing reliable predictions of cold-hardiness for various plant species under global climate warming.

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