scholarly journals Label-free quantitative proteomics and SAINT analysis enable interactome mapping for the human Ser/Thr protein phosphatase 5

PROTEOMICS ◽  
2011 ◽  
Vol 11 (8) ◽  
pp. 1508-1516 ◽  
Author(s):  
Dana V. Skarra ◽  
Marilyn Goudreault ◽  
Hyungwon Choi ◽  
Michael Mullin ◽  
Alexey I. Nesvizhskii ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Quantitative Proteomics ◽  
Label Free ◽  
Protein Phosphatase 5 ◽  
Interactome Mapping

Development of Algorithms for Mass Spectrometry-based Label-free Quantitative Proteomics*

2011 ◽  
Vol 38 (6) ◽  
pp. 506-518 ◽  
Author(s):  
Wei ZHANG ◽  
Ji-Yang ZHANG ◽  
Hui LIU ◽  
Han-Chang SUN ◽  
Chang-Ming XU ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Proteomics ◽  
Label Free

scholarly journals Overexpression of protein phosphatase 5 in the mouse heart: Reduced contractility but increased stress tolerance – Two sides of the same coin?

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221289 ◽  
Author(s):  
Ulrich Gergs ◽  
Tina Jahn ◽  
Franziska Werner ◽  
Carolin Köhler ◽  
Friedrich Köpp ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Protein Phosphatase ◽  
Mouse Heart ◽  
Protein Phosphatase 5 ◽  
Two Sides

scholarly journals Dephosphorylation of Tau by Protein Phosphatase 5

2004 ◽  
Vol 280 (3) ◽  
pp. 1790-1796 ◽  
Author(s):  
Fei Liu ◽  
Khalid Iqbal ◽  
Inge Grundke-Iqbal ◽  
Sandra Rossie ◽  
Cheng-Xin Gong
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 5

scholarly journals The Subcellular Localization of Plant Protein Phosphatase 5 Isoforms Is Determined by Alternative Splicing

2003 ◽  
Vol 133 (2) ◽  
pp. 702-712 ◽  
Author(s):  
Sergio de la Fuente van Bentem ◽  
Jack H. Vossen ◽  
Josephus E.M. Vermeer ◽  
Marianne J. de Vroomen ◽  
Theodorus W.J. Gadella ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Subcellular Localization ◽  
Protein Phosphatase ◽  
Plant Protein ◽  
Protein Phosphatase 5

scholarly journals Temporal Quantitative Proteomics Analysis of Neuroblastoma Cells Treated with Bovine Milk-Derived Extracellular Vesicles Highlights the Anti-Proliferative Properties of Milk-Derived Extracellular Vesicles

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 750
Author(s):  
Pamali Fonseka ◽  
Taeyoung Kang ◽  
Sing Chee ◽  
Sai V. Chitti ◽  
Rahul Sanwlani ◽  
...  
Keyword(s):  
High Risk ◽  
Extracellular Vesicles ◽  
Quantitative Proteomics ◽  
Cell Metabolism ◽  
Bovine Milk ◽  
Neuroblastoma Cells ◽  
Treatment Strategies ◽  
Treatment Resistance ◽  
Label Free ◽  
The Impact

Neuroblastoma (NBL) is a pediatric cancer that accounts for 15% of childhood cancer mortality. Amplification of the oncogene N-Myc occurs in 20% of NBL patients and is considered high risk as it correlates with aggressiveness, treatment resistance and poor prognosis. Even though the treatment strategies have improved in the recent years, the survival rate of high-risk NBL patients remain poor. Hence, it is crucial to explore new therapeutic avenues to sensitise NBL. Recently, bovine milk-derived extracellular vesicles (MEVs) have been proposed to contain anti-cancer properties. However, the impact of MEVs on NBL cells is not understood. In this study, we characterised MEVs using Western blotting, NTA and TEM. Importantly, treatment of NBL cells with MEVs decreased the proliferation and increased the sensitivity of NBL cells to doxorubicin. Temporal label-free quantitative proteomics of NBL cells highlighted the depletion of proteins involved in cell metabolism, cell growth and Wnt signalling upon treatment with MEVs. Furthermore, proteins implicated in cellular senescence and apoptosis were enriched in NBL cells treated with MEVs. For the first time, this study highlights the temporal proteomic profile that occurs in cancer cells upon MEVs treatment.

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