scholarly journals Layered electrophoretic transfer - A method for pre-analytic processing of histological sections

PROTEOMICS ◽  
2011 ◽  
Vol 11 (5) ◽  
pp. 883-889 ◽  
Author(s):  
Liang Zhu ◽  
Michael A. Tangrea ◽  
Sumana Mukherjee ◽  
Michael R. Emmert-Buck
Keyword(s):  
Histological Sections ◽  
Analytic Processing ◽  
Electrophoretic Transfer

Mentha pulegium Aqueous Extract Exhibits Antidiabetic and Hepatoprotective Effects in Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (3) ◽  
pp. 292-301
Author(s):  
Omar Farid ◽  
Naoufel Ali Zeggwagh ◽  
Fadwa EL Ouadi ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Aqueous Extract ◽  
Morphometric Analysis ◽  
Diabetic Rats ◽  
Mentha Pulegium ◽  
Glucose Levels ◽  
Histological Sections ◽  
Blood Glucose Levels ◽  
Liver And Pancreas

Objective: The aim of this work was to evaluate the antihyperglycemic activity of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rat. The glucose tolerance was evaluated in normal rats. Moreover, the histological sections and morphometric analysis at the liver and pancreas have been carried out in this investigation both in normal and STZ-diabetic rats. Methods: The effect of A.P.A.E of M. pulegium (20 mg/kg) on blood glucose levels was investigated in normal and diabetic rats (n=6). Histopathological changes in liver and pancreas were examined under phase contrast microscope and a preliminary screening for various bioactive constituents was realized according to standard methods. Key Findings: Both single and repeated oral administration of A.P.A.E (20 mg/kg) caused a significant reduction in blood glucose levels in STZ-diabetic rats (p<0.0001). The morphometric analysis and histological sections realized in pancreas and liver have showed the beneficial effect of the A.P.A.E in cellular population. According to oral glucose tolerance test (OGTT), the aqueous extract has revealed an improvement of glucose tolerance in normal rat. Furthermore, the preliminary phytochemical screening of A.P.A.E of M. pulegium has demonstrated the presence of various metabolite compounds including polyphenols, flavonoids, terpenoids tannins, cyanidins, sesquiterpenes, and glycosides. Conclusion: We conclude that the A.P.A.E of M. pulegium (20 mg/kg) exhibits a potent antihyperglycemic activity in STZ diabetic rats.

scholarly journals Automatic morphometry of nerve histological sections

2000 ◽  
Vol 97 (2) ◽  
pp. 111-122 ◽  
Author(s):  
E Romero ◽  
O Cuisenaire ◽  
J.F Denef ◽  
J Delbeke ◽  
B Macq ◽  
...  
Keyword(s):  
Histological Sections

scholarly journals Individual differences in strategy use and performance during fault diagnosis

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Michael Shreeves ◽  
Leo Gugerty ◽  
DeWayne Moore
Keyword(s):  
Individual Differences ◽  
Fault Diagnosis ◽  
Diagnostic Tests ◽  
Causal Reasoning ◽  
Fluid Intelligence ◽  
Positive Thinking ◽  
Reasoning Task ◽  
Thinking Dispositions ◽  
Analytic Processing ◽  
Heuristic Processing

Abstract Background Research on causal reasoning often uses group-level data analyses that downplay individual differences and simple reasoning problems that are unrepresentative of everyday reasoning. In three empirical studies, we used an individual differences approach to investigate the cognitive processes people used in fault diagnosis, which is a complex diagnostic reasoning task. After first showing how high-level fault diagnosis strategies can be composed of simpler causal inferences, we discussed how two of these strategies—elimination and inference to the best explanation (IBE)—allow normative performance, which minimizes the number of diagnostic tests, whereas backtracking strategies are less efficient. We then investigated whether the use of normative strategies was infrequent and associated with greater fluid intelligence and positive thinking dispositions and whether normative strategies used slow, analytic processing while non-normative strategies used fast, heuristic processing. Results Across three studies and 279 participants, uses of elimination and IBE were infrequent, and most participants used inefficient backtracking strategies. Fluid intelligence positively predicted elimination and IBE use but not backtracking use. Positive thinking dispositions predicted avoidance of backtracking. After classifying participants into groups that consistently used elimination, IBE, and backtracking, we found that participants who used elimination and IBE made fewer, but slower, diagnostic tests compared to backtracking users. Conclusions Participants’ fault diagnosis performance showed wide individual differences. Use of normative strategies was predicted by greater fluid intelligence and more open-minded and engaged thinking dispositions. Elimination and IBE users made the slow, efficient responses typical of analytic processing. Backtracking users made the fast, inefficient responses suggestive of heuristic processing.

scholarly journals Multi-center clinical study using optical coherence tomography for evaluation of cervical lesions in-vivo

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chenchen Ren ◽  
Xianxu Zeng ◽  
Zhongna Shi ◽  
Chunyan Wang ◽  
Huifen Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Precancerous Lesions ◽  
Diagnostic Value ◽  
Optical Coherence ◽  
Cervical Lesions ◽  
In Vivo Evaluation ◽  
Histological Sections ◽  
Histological Images

AbstractIn this prospective study of an in-vivo cervical examination using optical coherence tomography (OCT), we evaluated the diagnostic value of non-invasive and real-time OCT in cervical precancerous lesions and cancer diagnosis, and determined the characteristics of OCT images. 733 patients from 5 Chinese hospitals were inspected with OCT and colposcopy-directed biopsy. The OCT images were compared with the histological sections to find out the characteristics of various categories of lesions. The OCT images were also interpreted by 3 investigators to make a 2-class classification, and the results were compared against the pathological results. Various structures of the cervical tissue were clearly observed in OCT images, which matched well with the corresponding histological sections. The OCT diagnosis results delivered a sensitivity of 87.0% (95% confidence interval, CI 82.2–90.7%), a specificity of 84.1% (95% CI 80.3–87.2%), and an overall accuracy of 85.1%. Both good consistency of OCT images and histological images and satisfactory diagnosis results were provided by OCT. Due to its features of non-invasion, real-time, and accuracy, OCT is valuable for the in-vivo evaluation of cervical lesions and has the potential to be one of the routine cervical diagnosis methods.

Dysprosium-enhanced MR imaging for tumor tissue characterization

1997 ◽  
Vol 38 (2) ◽  
pp. 281-286 ◽  
Author(s):  
C. Wang ◽  
A. Sundin ◽  
A. Ericsson ◽  
T. Bach-Gansmo ◽  
A. Hemmingsson ◽  
...  
Keyword(s):  
Mr Imaging ◽  
Tissue Characterization ◽  
Tumor Tissue ◽  
Tumor Model ◽  
Human Colon ◽  
Mr Images ◽  
Histological Sections ◽  
Necrotic Tumor ◽  
Before And After ◽  
Weighted Sequences

Purpose: to evaluate dysprosium-enhanced MR imaging for differentiation between morphologically intact and necrotic tumor tissue in a tumor model. Material and Methods: A human colon carcinoma was transplanted subcutaneously into 9 nude (immunodeprived) rats. MR imaging was performed before and after injection of the dysprosium agent Dy-DTPA-BMA. T1-, T2- and T2*-weighted sequences were acquired. the tumors were dissected, histological sections were prepared, and compared with corresponding MR images. Results: in intact tissue, the MR signal intensity in the T2- and T2*-weighted images decreased after Dy injection and the delineation of the intact regions were sharp and corresponded well to the gross histological sections. Conclusion: Dy-enhanced MR imaging facilitated the differentiation between intact and necrotic tumor tissue.

Three-dimensional temporal bone reconstruction from histological sections

2014 ◽  
Vol 128 (5) ◽  
pp. 416-420 ◽  
Author(s):  
N Ahmad ◽  
A Wright
Keyword(s):  
High Resolution ◽  
Temporal Bone ◽  
Personal Computer ◽  
Three Dimensional ◽  
Jugular Bulb ◽  
Chorda Tympani Nerve ◽  
Three Dimensional Model ◽  
Histological Sections ◽  
Volumetric Reconstruction ◽  
Stapes Footplate

AbstractObjective:To produce a high-resolution, three-dimensional temporal bone model from serial sections, using a personal computer.Method:Digital images were acquired from histological sections of the temporal bone. Image registration, segmentation and three-dimensional volumetric reconstruction were performed using a personal computer. The model was assessed for anatomical accuracy and interactivity by otologists.Results:An accurate, high-resolution, three-dimensional model of the temporal bone was produced, containing structures relevant to otological surgery. The facial nerve, labyrinth, internal carotid artery, jugular bulb and all of the ossicles were seen (including the stapes footplate), together with the internal and external auditory meati. Some projections also showed the chorda tympani nerve.Conclusion:A high-resolution, three-dimensional computer model of the complete temporal bone was produced using a personal computer. Because of the increasing difficulty in procuring cadaveric bones, this model could be a useful adjunct for training.

scholarly journals Affinity purification and subunit structures of β-N-acetylhexosaminidases A and B from boar epididymis

1984 ◽  
Vol 219 (3) ◽  
pp. 1009-1015 ◽  
Author(s):  
H C Parkes ◽  
J L Stirling ◽  
P Calvo
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Affinity Purification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Deae Cellulose ◽  
Gradient Gel Electrophoresis ◽  
Sepharose 4B ◽  
Electrophoretic Transfer ◽  
Peptide Maps

beta-N-Acetylhexosaminidase from boar epididymis was separated into two forms, A and B, on DEAE-cellulose. Both these forms were excluded from Sepharose S-200 and had apparent Mr values of 510 000 on gradient gel electrophoresis under non-denaturing conditions. Affinity chromatography on 2-acetamido-N-(6-aminohexanoyl)-2-deoxy-beta-D-glucopyranosylam ine coupled to CNBr-activated Sepharose 4B was used to separate and purify beta-N-acetylhexosaminidases A and B that had specific activities of 115 and 380 mumol/min per mg of protein respectively. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of denatured beta-N-acetylhexosaminidase A gave a single major component of Mr 67 000. beta-N-Acetylhexosaminidase B also had this component, and in addition had polypeptides of Mr 29 000 and 26 000. All these polypeptides were glycosylated. Antiserum to the B form precipitated form A from solution and reacted with the 67 000-Mr component or form A after electrophoretic transfer from sodium dodecyl sulphate/polyacrylamide gels to nitrocellulose sheets. The 67 000-Mr components of forms A and B yielded identical peptide maps when digested with Staphylococcus aureus V8 proteinase, and the 29 000-Mr and 26 000-Mr components in form B may be related to the 67 000-Mr polypeptide.

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