scholarly journals A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates

PROTEOMICS ◽  
2009 ◽  
Vol 9 (5) ◽  
pp. 1416-1416
Author(s):  
Noor Remmerie ◽  
Luc Roef ◽  
Eveline Van De Slijke ◽  
Jelle Van Leene ◽  
Geert Persiau ◽  
...  
Keyword(s):  
Plant Cell ◽  
Protein Complexes ◽  
Bioanalytical Method ◽  
Cell Lysates ◽  
Whole Plant

A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates

PROTEOMICS ◽  
2009 ◽  
Vol 9 (3) ◽  
pp. 598-609 ◽  
Author(s):  
Noor Remmerie ◽  
Luc Roef ◽  
Eveline Van De Slijke ◽  
Jelle Van Leene ◽  
Geert Persiau ◽  
...  
Keyword(s):  
Plant Cell ◽  
Protein Complexes ◽  
Bioanalytical Method ◽  
Cell Lysates ◽  
Whole Plant

Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry

2019 ◽  
Author(s):  
Zachary VanAernum ◽  
Florian Busch ◽  
Benjamin J. Jones ◽  
Mengxuan Jia ◽  
Zibo Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Speed ◽  
Structural Information ◽  
Protein Complexes ◽  
High Sensitivity ◽  
Native Mass Spectrometry ◽  
Structural Features ◽  
Consumer Products ◽  
Cell Lysates ◽  
Protein Expression And Purification

It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer products, chemical processes, and therapeutics. Native mass spectrometry (nMS) has become an important tool in protein analysis due to its ability to retain non-covalent interactions during measurements, making it possible to obtain protein structural information with high sensitivity and at high speed. Interferences from the presence of non-volatiles are typically alleviated by offline buffer exchange, which is timeconsuming and difficult to automate. We provide a protocol for rapid online buffer exchange (OBE) nMS to directly screen structural features of pre-purified proteins, protein complexes, or clarified cell lysates. Information obtained by OBE nMS can be used for fast (<5 min) quality control and can further guide protein expression and purification optimization.

Chemical characterisation of the whole plant cell wall of archaeological wood: an integrated approach

2017 ◽  
Vol 409 (17) ◽  
pp. 4233-4245 ◽  
Author(s):  
Luca Zoia ◽  
Diego Tamburini ◽  
Marco Orlandi ◽  
Jeannette Jacqueline Łucejko ◽  
Anika Salanti ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Integrated Approach ◽  
Archaeological Wood ◽  
Whole Plant

scholarly journals Rapid Online Buffer Exchange: A Method for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry

2019 ◽  
Author(s):  
Zachary VanAernum ◽  
Florian Busch ◽  
Benjamin J. Jones ◽  
Mengxuan Jia ◽  
Zibo Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Speed ◽  
Structural Information ◽  
Protein Complexes ◽  
High Sensitivity ◽  
Native Mass Spectrometry ◽  
Structural Features ◽  
Consumer Products ◽  
Cell Lysates ◽  
Protein Expression And Purification

It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in consumer products, chemical processes, and therapeutics. Native mass spectrometry (nMS) has become an important tool in protein analysis due to its ability to retain non-covalent interactions during measurements, making it possible to obtain protein structural information with high sensitivity and at high speed. Interferences from the presence of non-volatiles are typically alleviated by offline buffer exchange, which is timeconsuming and difficult to automate. We provide a protocol for rapid online buffer exchange (OBE) nMS to directly screen structural features of pre-purified proteins, protein complexes, or clarified cell lysates. Information obtained by OBE nMS can be used for fast (<5 min) quality control and can further guide protein expression and purification optimization.

Cross-linking approach to affinity capture of protein complexes from chaotrope-solubilized cell lysates

2004 ◽  
Vol 324 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Iraide Alloza ◽  
Erik Martens ◽  
Susan Hawthorne ◽  
Koen Vandenbroeck
Keyword(s):  
Protein Complexes ◽  
Cross Linking ◽  
Affinity Capture ◽  
Cell Lysates

scholarly journals The effect of exogenous factors on the polyenzymatic activity of RuBisCO and ATP synthase of chloroplasts from pea leaves

2021 ◽  
Vol 19 (3(75)) ◽  
pp. 21-27
Author(s):  
Andriy V. Semenikhin ◽  
Volodymyr V. Sukhovieiev ◽  
Mykola V. Patyka ◽  
Vasyl S. Lukach
Keyword(s):  
Carbonic Anhydrase ◽  
Atpase Activity ◽  
Plant Cell ◽  
Atp Synthase ◽  
Protein Complexes ◽  
Polyacrylamide Gel ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Exogenous Origin ◽  
Dimensional Electrophoresis

Aim. To isolate and purify protein complexes – ATP synthase and RuBisCO – from pea leaf chloroplasts and study the effect of a microbiological fertilizer “Extracon” and sulfonamide inhibitors acetazolamide and ethoxyzolamide on the enzymatic activity of these proteins.Materials and methods. Chloroplasts were isolated from the leaves of two-week-old pea sprouts, protein complexes of purified thylakoid membranes were solubilized with digitonin (10 mg of digitonin per 1 mg of protein), the protein concentration was determined according to Lowry. Native electrophoresis with displacement of the charge of the soluble protein fraction from the chloroplast stroma, as well as membrane proteins, was carried out in the modified system of Anderson et al., Kolisnichenko et al. A modified Lemmley system was applied to the protein electrophoresis in the polyacrylamide gel in the presence of sodium dodecyl sulfate. The methods of Alain and Hintsik, as well as Gomorrah were used to determine the ATPase activity in the polyacrylamide gel. Visualization of the carbonic anhydrase activity in the polyacrylamide gel was performed by the method of Edwards and Petton. Results and discussion. Using physicochemical methods of potentiometry, spectrophotometry the ATPase, carbonic anhydrase and esterase activities of the enzymes were studied. The results obtained indicate that specific carbonic anhydrase inhibitors (acetazolamide and ethoxyzolamide) also block the esterase and ATPase activity of the enzyme complexes. “Extracon” (a multifunctional microbiological preparation) almost 1.5 times increases the activity of the enzymes, showing a complex activating effect of the fertilizer on both light and dark reactions of photosynthesis.Conclusions. The method of identification and isolation of RuBisCO and ATP synthase on the basis of two-dimensional electrophoresis and electrophoretic elution has been proposed. It allows determining the presence of certain enzyme activity of complexes at first in SDS plates (express analysis) and further to study the effect of various factors of endogenous and exogenous origin on the enzymatic properties of electrophoretically pure enzymes. The use of two-dimensional electrophoresis as a tool for assessing the impact of various factors of endogenous and exogenous origin on the plant cell and the plant as a whole through constant monitoring of the work and activity of enzyme systems of the plant cell is promising.

scholarly journals Asymmetry of Plant Cell Divisions under Salt Stress

Symmetry ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1811
Author(s):  
Ekaterina N. Baranova ◽  
Alexander A. Gulevich
Keyword(s):  
Salt Stress ◽  
Plant Cell ◽  
Normal Growth ◽  
Cell Content ◽  
Daughter Cells ◽  
Whole Plant ◽  
Unique System ◽  
Significant Damage ◽  
Typical Shape ◽  
Cellular Compartments

Salt stress causes several damaging effects in plant cells. These commonly observed effects are the results of oxidative, osmotic, and toxic stresses. To ensure normal growth and development of tissues, the cellular compartments of multicellular plants have a unique system that provides the specified parameters of growth and differentiation. The cell shape and the direction of division support the steady development of the organism, the habit, and the typical shape of the organs and the whole plant. When dividing, daughter cells evenly or unevenly distribute the components of cytoplasm. Factors such as impaired osmotic regulation, exposure to toxic compounds, and imbalance in the antioxidant system cause disorders associated with the moving of organelles, distribution transformations of the endoplasmic reticulum, and the vacuolar compartment. In some cases, one can observe a different degree of plasmolysis manifestation, local changes in the density of cytoplasm. Together, these processes can cause disturbances in the direction of cell division, the formation of a phragmoplast, the formation of nuclei of daughter cells, and a violation of their fine structural organization. These processes are often accompanied by significant damage to the cytoskeleton, the formation of nonspecific structures formed by proteins of the cytoskeleton. The consequences of these processes can lead to the death of some cells or to a significant change in their morphology and properties, deformation of newly formed tissues and organs, and changes in the plant phenotype. Thus, as a result of significant violations of the cytoskeleton, causing critical destabilization of the symmetric distribution of the cell content, disturbances in the distribution of chromosomes, especially in polyploid cells, may occur, resulting in the appearance of micronuclei. Hence, the asymmetry of a certain component of the plant cell is a marker of susceptibility to abiotic damage.

Animal and Plant Cell Lysates Share a Conserved Chaperone System That Assembles the Glucocorticoid Receptor into a Functional Heterocomplex with hsp90†

Biochemistry ◽  
1996 ◽  
Vol 35 (2) ◽  
pp. 554-561 ◽  
Author(s):  
Louis F. Stancato ◽  
Kevin A. Hutchison ◽  
Priti Krishna ◽  
William B. Pratt
Keyword(s):  
Glucocorticoid Receptor ◽  
Plant Cell ◽  
Cell Lysates

scholarly journals Mild Acetylation and Solubilization of Ground Whole Plant Cell Walls in EmimAc: A Method for Solution-State NMR in DMSO-d6

2020 ◽  
Vol 92 (19) ◽  
pp. 13101-13109
Author(s):  
Xin Liu ◽  
Ruonan Zhu ◽  
Tianying Chen ◽  
Pingping Song ◽  
Fachuang Lu ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Plant Cell Walls ◽  
Solution State ◽  
Whole Plant
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