Combining selected reaction monitoring with discovery proteomics in limited biological samples

PROTEOMICS ◽  
2009 ◽  
Vol 9 (21) ◽  
pp. 4834-4836 ◽  
Author(s):  
Mukesh Kumar Gupta ◽  
Jin Woo Jung ◽  
Sang Jun Uhm ◽  
Hookeun Lee ◽  
Hoon Taek Lee ◽  
...  
Keyword(s):  
Biological Samples ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring

scholarly journals Algorithm of targeted (SRM) methods development in mass-spectrometric studies

2018 ◽  
Vol 1 (1) ◽  
pp. e00006 ◽  
Author(s):  
A.T. Kopylov ◽  
A.V. Lisitsa ◽  
V.G. Zgoda
Keyword(s):  
Biological Samples ◽  
Protein Concentration ◽  
Clinical Biochemistry ◽  
Protein Detection ◽  
Correct Choice ◽  
Selected Reaction Monitoring ◽  
Biological Research ◽  
Reaction Monitoring ◽  
Methods Development

Determination of protein concentration in biological samples is an important task for biological research, as well as for medicine and routine clinical biochemistry. The introduction of stable isotope-labeled peptide standards (SIS) made it possible to determine accurately absolute protein concentrations in proteomic studies. The correct choice of SIS and the systematic way to develop a s method for selected reaction monitoring (SRM) are very important steps that are crucial for further identification and measurements of protein concentration. In this paper, we summarize our experience of selecting SIS for measuring the protein concentration by SRM. The results are presented in the form of an algorithm that describes the main stages of the SIS selection and the main points in the development of SRM methods for the targeted protein detection and determination of protein concentrations in biological samples.

Automated Workflow for Large-Scale Selected Reaction Monitoring Experiments

2012 ◽  
Vol 11 (3) ◽  
pp. 1644-1653 ◽  
Author(s):  
Lars Malmström ◽  
Johan Malmström ◽  
Nathalie Selevsek ◽  
George Rosenberger ◽  
Ruedi Aebersold
Keyword(s):  
Large Scale ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring
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