Proteomic analysis of Arabidopsis halleri shoots in response to the heavy metals cadmium and zinc and rhizosphere microorganisms

PROTEOMICS ◽  
2009 ◽  
Vol 9 (21) ◽  
pp. 4837-4850 ◽  
Author(s):  
Silvia Farinati ◽  
Giovanni DalCorso ◽  
Elisa Bona ◽  
Michela Corbella ◽  
Silvia Lampis ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Proteomic Analysis ◽  
Arabidopsis Halleri ◽  
Rhizosphere Microorganisms

scholarly journals Proteogenomic Analysis of Burkholderia Species Strains 25 and 46 Isolated from Uraniferous Soils Reveals Multiple Mechanisms to Cope with Uranium Stress

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 269 ◽  
Author(s):  
Meenakshi Agarwal ◽  
Ashish Pathak ◽  
Rajesh Rathore ◽  
Om Prakash ◽  
Rakesh Singh ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Stress Response ◽  
Proteomic Analysis ◽  
Protein Biosynthesis ◽  
Draft Genome ◽  
Department Of Energy ◽  
Coding Sequences ◽  
Total Rna ◽  
A Genome ◽  
Rna Genes

Two Burkholderia spp. (strains SRS-25 and SRS-46) were isolated from high concentrations of uranium (U) from the U.S. Department of Energy (DOE)-managed Savannah River Site (SRS). SRS contains soil gradients that remain co-contaminated by heavy metals from previous nuclear weapons production activities. Uranium (U) is one of the dominant contaminants within the SRS impacted soils, which can be microbially transformed into less toxic forms. We established microcosms containing strains SRS-25 and SRS-46 spiked with U and evaluated the microbially-mediated depletion with concomitant genomic and proteomic analysis. Both strains showed a rapid depletion of U; draft genome sequences revealed SRS-25 genome to be of approximately 8,152,324 bp, a G + C content of 66.5, containing a total 7604 coding sequences with 77 total RNA genes. Similarly, strain SRS-46 contained a genome size of 8,587,429 bp with a G + C content of 67.1, 7895 coding sequences, with 73 total RNA genes, respectively. An in-depth, genome-wide comparisons between strains 25, 46 and a previously isolated strain from our research (Burkholderia sp. strain SRS-W-2-2016), revealed a common pool of 3128 genes; many were found to be homologues to previously characterized metal resistance genes (e.g., for cadmium, cobalt, and zinc), as well as for transporter, stress/detoxification, cytochromes, and drug resistance functions. Furthermore, proteomic analysis of strains with or without U stress, revealed the increased expression of 34 proteins from strain SRS-25 and 52 proteins from strain SRS-46; similar to the genomic analyses, many of these proteins have previously been shown to function in stress response, DNA repair, protein biosynthesis and metabolism. Overall, this comparative proteogenomics study confirms the repertoire of metabolic and stress response functions likely rendering the ecological competitiveness to the isolated strains for colonization and survival in the heavy metals contaminated SRS soil habitat.

Heavy metals modulate DNA compaction and methylation at CpG sites in the metal hyperaccumulator Arabidopsis halleri

2021 ◽  
Author(s):  
Serena Galati ◽  
Mariolina Gullì ◽  
Gianluigi Giannelli ◽  
Antonella Furini ◽  
Giovanni DalCorso ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Arabidopsis Halleri ◽  
Dna Compaction ◽  
Cpg Sites

scholarly journals Plants accumulating heavy metals in the Sudety Mts

2011 ◽  
Vol 75 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Teresa Brej ◽  
Jerzy Fabiszewski
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Vascular Plants ◽  
Dry Weight ◽  
Systematic Position ◽  
Plant Biodiversity ◽  
Arabidopsis Halleri ◽  
Ore Deposit ◽  
Nickel Zinc ◽  
Armeria Maritima

The Sudeten flora consists of some plants we can recognize as heavy metal accumulators. Between others there are: <em>Thlaspi caerulescens</em>, <em>Arabidopsis halleri</em>, <em>Armeria maritima</em> ssp. <em>halleri</em> s.l. and probably the endemic fern <em>Asplenium onopteris</em> var. <em>silesiaca</em>. The authors present the concentrations of some important heavy metals measured in aboveground plant dry weight. The highest concentration of zinc was 8220 ppm (<em>Thlaspi</em>), nickel - 3100 ppm (<em>Thlaspi</em>), lead - 83 ppm (<em>Armeria</em>), copper - 611 ppm (<em>Arabidopsis</em>) and cadmium - 28 ppm (<em>Thlaspi</em>). The concentrations depend rather on species or population specification than on ore deposit quality. There are no typical hyperaccumulator among plants we have examined, but some signs of hyperaccumulation of nickel, zinc and lead could be observed. There are no typical endemic taxa, only <em>Asplenium onopteris</em> var. <em>silesiaca</em> and <em>Armeria maritima</em> ssp. <em>halleri</em> may be recognized as neoendemic taxa, but still of unclear systematic position. During the study we tried to find out why some Sudeten vascular plants do not develop heavy metals hyperaccumulation and why they are rather latent hyperaccumulators. Finally, we suggest to protect some metallicolous areas in spite they are rather territories with low plant biodiversity.

Proteomic analysis in Daphnia magna exposed to As(III), As(V) and Cd heavy metals and their binary mixtures for screening potential biomarkers

Chemosphere ◽  
2013 ◽  
Vol 93 (10) ◽  
pp. 2341-2348 ◽  
Author(s):  
Thai-Hoang Le ◽  
Eun-Suk Lim ◽  
Nam-Hui Hong ◽  
Sung-Kyu Lee ◽  
Yon Sik Shim ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Daphnia Magna ◽  
Binary Mixtures ◽  
Proteomic Analysis ◽  
Potential Biomarkers

scholarly journals Nuclear microsatellite loci for Arabidopsis halleri (Brassicaceae), a model species to study plant adaptation to heavy metals

2012 ◽  
Vol 99 (2) ◽  
pp. e49-e52 ◽  
Author(s):  
Cécile Godé ◽  
Isabelle Decombeix ◽  
Alicja Kostecka ◽  
Pawel Wasowicz ◽  
Maxime Pauwels ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Microsatellite Loci ◽  
Arabidopsis Halleri ◽  
Model Species ◽  
Plant Adaptation ◽  
Nuclear Microsatellite

Sampling and analysis of the air-water interface with SEM and EDS of microlayers

1989 ◽  
Vol 47 ◽  
pp. 1004-1005
Author(s):  
Randall W. Smith ◽  
John Dash
Keyword(s):  
Heavy Metals ◽  
Surface Microlayer ◽  
Surface Films ◽  
Water Interface ◽  
Physical Processes ◽  
Infrared Spectroscopic Analysis ◽  
Eds Analysis ◽  
Air Water Interface ◽  
Significant Area ◽  
Bulk Chemical

The structure of the air-water interface forms a boundary layer that involves biological ,chemical geological and physical processes in its formation. Freshwater and sea surface microlayers form at the air-water interface and include a diverse assemblage of organic matter, detritus, microorganisms, plankton and heavy metals. The sampling of microlayers and the examination of components is presently a significant area of study because of the input of anthropogenic materials and their accumulation at the air-water interface. The neustonic organisms present in this environment may be sensitive to the toxic components of these inputs. Hardy reports that over 20 different methods have been developed for sampling of microlayers, primarily for bulk chemical analysis. We report here the examination of microlayer films for the documentation of structure and composition.Baier and Gucinski reported the use of Langmuir-Blogett films obtained on germanium prisms for infrared spectroscopic analysis (IR-ATR) of components. The sampling of microlayers has been done by collecting fi1ms on glass plates and teflon drums, We found that microlayers could be collected on 11 mm glass cover slips by pulling a Langmuir-Blogett film from a surface microlayer. Comparative collections were made on methylcel1ulose filter pads. The films could be air-dried or preserved in Lugol's Iodine Several slicks or surface films were sampled in September, 1987 in Chesapeake Bay, Maryland and in August, 1988 in Sequim Bay, Washington, For glass coverslips the films were air-dried, mounted on SEM pegs, ringed with colloidal silver, and sputter coated with Au-Pd, The Langmuir-Blogett film technique maintained the structure of the microlayer intact for examination, SEM observation and EDS analysis were then used to determine organisms and relative concentrations of heavy metals, using a Link AN 10000 EDS system with an ISI SS40 SEM unit. Typical heavy microlayer films are shown in Figure 3.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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