The Fusarium oxysporum cell wall proteome under adhesion-inducing conditions

PROTEOMICS ◽  
2009 ◽  
Vol 9 (20) ◽  
pp. 4755-4769 ◽  
Author(s):  
Rafael Prados-Rosales ◽  
Jose L. Luque-Garcia ◽  
Raquel Martínez-López ◽  
Concha Gil ◽  
Antonio Di Pietro
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Cell Wall Proteome

In vitro Cell Wall Stress Assay for Fusarium oxysporum

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (17) ◽  
Author(s):  
Elena Pérez-Nadales ◽  
Antonio Di Pietro
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Wall Stress ◽  
Cell Wall Stress

Rho1 has distinct functions in morphogenesis, cell wall biosynthesis and virulence of Fusarium oxysporum

2008 ◽  
Vol 10 (6) ◽  
pp. 1339-1351 ◽  
Author(s):  
Ana Lilia Martínez-Rocha ◽  
M. Isabel G. Roncero ◽  
Adriana López-Ramirez ◽  
Marçal Mariné ◽  
Josep Guarro ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Cell Wall Biosynthesis

scholarly journals The Transcription Factor Con7-1 Is a Master Regulator of Morphogenesis and Virulence in Fusarium oxysporum

2015 ◽  
Vol 28 (1) ◽  
pp. 55-68 ◽  
Author(s):  
Carmen Ruiz-Roldán ◽  
Yolanda Pareja-Jaime ◽  
José Antonio González-Reyes ◽  
M. Isabel G. Roncero
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Gene Expression Analysis ◽  
Wall Structure ◽  
Targeted Deletion ◽  
Signal Perception ◽  
Cell Wall Biogenesis ◽  
Primary And Secondary Metabolism ◽  
Genes Encoding

Previous studies have demonstrated the essential role of morphogenetic regulation in Fusarium oxysporum pathogenesis, including processes such as cell-wall biogenesis, cell division, and differentiation of infection-like structures. We identified three F. oxysporum genes encoding predicted transcription factors showing significant identities to Magnaporthe oryzae Con7p, Con7-1, plus two identical copies of Con7-2. Targeted deletion of con7-1 produced nonpathogenic mutants with altered morphogenesis, including defects in cell wall structure, polar growth, hyphal branching, and conidiation. By contrast, simultaneous inactivation of both con7-2 copies caused no detectable defects in the resulting mutants. Comparative microarray-based gene expression analysis indicated that Con7-1 modulates the expression of a large number of genes involved in different biological functions, including host–pathogen interactions, morphogenesis and development, signal perception and transduction, transcriptional regulation, and primary and secondary metabolism. Taken together, our results point to Con7-1 as general regulator of morphogenesis and virulence in F. oxysporum.

Cell wall proteome analysis of banana fruit softening using iTRAQ technology

2019 ◽  
Vol 209 ◽  
pp. 103506 ◽  
Author(s):  
Lu Xiao ◽  
Taotao Li ◽  
Guoxiang Jiang ◽  
Yueming Jiang ◽  
Xuewu Duan
Keyword(s):  
Cell Wall ◽  
Proteome Analysis ◽  
Banana Fruit ◽  
Fruit Softening ◽  
Cell Wall Proteome

scholarly journals The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems

Biology ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 60 ◽  
Author(s):  
Sergeant ◽  
Printz ◽  
Guerriero ◽  
Renaut ◽  
Lutts ◽  
...  
Keyword(s):  
Cell Wall ◽  
Apical Region ◽  
Stem Segment ◽  
Burp Domain ◽  
Composition And Structure ◽  
Striking Change ◽  
Stem Development ◽  
Cell Wall Proteome ◽  
Beta Galactosidase

In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared. Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained. This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure. The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis. Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region. Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region. Beta-amylase and ferritin likewise accumulate more in the basal stem segment. Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment. The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit. However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.

Arabidopsis cell wall proteome defined using multidimensional protein identification technology

PROTEOMICS ◽  
2006 ◽  
Vol 6 (1) ◽  
pp. 301-311 ◽  
Author(s):  
Emmanuelle M. Bayer ◽  
Andrew R. Bottrill ◽  
John Walshaw ◽  
Marielle Vigouroux ◽  
Mike J. Naldrett ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Identification ◽  
Multidimensional Protein Identification Technology ◽  
Cell Wall Proteome

scholarly journals Comparative Transcriptomic Analysis of Race 1 and Race 4 of Fusarium oxysporum f. sp. cubense Induced with Different Carbon Sources

2017 ◽  
Vol 7 (7) ◽  
pp. 2125-2138 ◽  
Author(s):  
Shiwen Qin ◽  
Chunyan Ji ◽  
Yunfeng Li ◽  
Zhenzhong Wang
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Host Cell ◽  
Expression Profiles ◽  
Cell Wall Polysaccharide ◽  
Host Cell Wall ◽  
Race 1 ◽  
Race 4 ◽  
Banana Cultivar

Abstract The fungal pathogen Fusarium oxysporum f. sp. cubense causes Fusarium wilt, one of the most destructive diseases in banana and plantain cultivars. Pathogenic race 1 attacks the “Gros Michel” banana cultivar, and race 4 is pathogenic to the Cavendish banana cultivar and those cultivars that are susceptible to Foc1. To understand the divergence in gene expression modules between the two races during degradation of the host cell wall, we performed RNA sequencing to compare the genome-wide transcriptional profiles of the two races grown in media containing banana cell wall, pectin, or glucose as the sole carbon source. Overall, the gene expression profiles of Foc1 and Foc4 in response to host cell wall or pectin appeared remarkably different. When grown with host cell wall, a much larger number of genes showed altered levels of expression in Foc4 in comparison with Foc1, including genes encoding carbohydrate-active enzymes (CAZymes) and other virulence-related genes. Additionally, the levels of gene expression were higher in Foc4 than in Foc1 when grown with host cell wall or pectin. Furthermore, a great majority of genes were differentially expressed in a variety-specific manner when induced by host cell wall or pectin. More specific CAZymes and other pathogenesis-related genes were expressed in Foc4 than in Foc1 when grown with host cell wall. The first transcriptome profiles obtained for Foc during degradation of the host cell wall may provide new insights into the mechanism of banana cell wall polysaccharide decomposition and the genetic basis of Foc host specificity.

The cell wall proteome from two strains of Pseudocercospora fijiensis with differences in virulence

2019 ◽  
Vol 35 (7) ◽  
Author(s):  
Yamily Y. Burgos-Canul ◽  
Blondy Canto-Canché ◽  
Maxim V. Berezovski ◽  
Gleb Mironov ◽  
Víctor M. Loyola-Vargas ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Wall Proteome ◽  
Pseudocercospora Fijiensis

Dual-specificity protein phosphatase Msg5 controls cell wall integrity and virulence in Fusarium oxysporum

2021 ◽  
Vol 146 ◽  
pp. 103486
Author(s):  
Tânia R. Fernandes ◽  
Encarnación Sánchez Salvador ◽  
Ángela G. Tapia ◽  
Antonio Di Pietro
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Protein Phosphatase ◽  
Cell Wall Integrity ◽  
Dual Specificity ◽  
Dual Specificity Protein Phosphatase ◽  
Specificity Protein
Sign in / Sign up

Export Citation Format

Share Document