Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalis

PROTEOMICS ◽  
2009 ◽  
Vol 10 (3) ◽  
pp. 555-563 ◽  
Author(s):  
Christina Liedert ◽  
Jörg Bernhardt ◽  
Dirk Albrecht ◽  
Birgit Voigt ◽  
Michael Hecker ◽  
...  
Keyword(s):  
Resistant Bacterium ◽  
Two Dimensional ◽  
Deinococcus Geothermalis ◽  
Radiation Resistant ◽  
Reference Map

scholarly journals An Antioxidant Defense System in Radiation-Resistant Bacterium Deinococcus geothermalis against Oxidative Stress

2021 ◽  
Author(s):  
Chanjae Lee ◽  
Min K. Bae ◽  
Sung-Jae Lee
Keyword(s):  
Oxidative Stress ◽  
Dna Binding ◽  
Carotenoid Biosynthesis ◽  
Antioxidant Defense System ◽  
Redox Balance ◽  
Primary Response ◽  
Resistant Bacterium ◽  
Loss Of Function ◽  
Deinococcus Geothermalis ◽  
Radiation Resistant

A radiation-resistant bacterium, Deinococcus geothermalis has various stress response mechanisms, including antioxidation. Features that maintain vitality at high radiation doses include the following: enzymatic scavengers of ROS such as catalase, SOD, and peroxidase; strain-specific DNA repair systems such as Deinococcal unique proteins; non-enzymatic responses such as manganese complexes, carotenoids, and DNA-binding proteins. This chapter summarizes the primary response mechanism by redox balance centered on the cystine transporter. It also reviews action characteristics of DNA-binding protein Dps and a putative LysR family protein, and effects on loss of function of the carotenoid biosynthesis genes by transposition of insertion sequences. Environmental adaptation and molecular evolution of radiation-resistant bacterium are also considered to explain the potentials of molecular behavior induced by oxidative stress.

scholarly journals Genome Plasticity by Insertion Sequences Learned From a Case of Radiation-Resistant Bacterium Deinococcus geothermalis

2021 ◽  
Vol 15 ◽  
pp. 117793222110374
Author(s):  
Chanjae Lee ◽  
Min K Bae ◽  
Nakjun Choi ◽  
Su Jeong Lee ◽  
Sung-Jae Lee
Keyword(s):  
Bacterial Genome ◽  
Single Species ◽  
Resistant Bacterium ◽  
Insertion Sequences ◽  
Genome Plasticity ◽  
Total Size ◽  
Deinococcus Geothermalis ◽  
Radiation Resistant ◽  
Is Integration ◽  
Main Chromosome

The genome of the radiation-resistant bacterium Deinococcus geothermalis contains 19 types of insertion sequences (ISs), including 93 total transposases (Tpases) in 73 full-length ISs from the main chromosome and 2 mega plasmids. In this study, 68 ISs from the D. geothermalis genome were extracted to implicate the earlier genome before its mutation by transposition of ISs. The total size of eliminated ISs from genome was 78.85 kb. From these in silico corrections of mutation by the ISs, we have become aware of some bioinformatics factualness as follows: (1) can reassemble the disrupted genes if the exact IS region was eliminated, (2) can configure the schematic clustering of major DDE type Tpases, (3) can determine IS integration order across multiple hot spots, and (4) can compare genetic relativeness by the partial synteny analysis between D. geothermalis and Deinococcus strain S9. Recently, we found that several IS elements actively transferred to other genomic sites under hydrogen peroxide-induced oxidative stress conditions, resulting in the inactivation of functional genes. Therefore, the single species genome’s mobilome study provides significant support to define bacterial genome plasticity and molecular evolution from past and present progressive transposition events.

Complete genome sequence of Microvirga sp. 17mud 1–3, a radiation-resistant bacterium

2018 ◽  
Vol 14 (3) ◽  
pp. 347-352 ◽  
Author(s):  
Gayathri Sathiyaraj ◽  
Myung Kyum Kim ◽  
Ju-Young Kim ◽  
Su-Jeong Kim ◽  
Jun Hwee Jang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Resistant Bacterium ◽  
Radiation Resistant

Deinococcus swuensis sp. nov., a gamma-radiation-resistant bacterium isolated from soil

2013 ◽  
Vol 51 (3) ◽  
pp. 305-311 ◽  
Author(s):  
Jae-Jin Lee ◽  
Hyun Ji Lee ◽  
Gi Seon Jang ◽  
Ja Myoung Yu ◽  
Ji Yoon Cha ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Resistant Bacterium ◽  
Radiation Resistant

scholarly journals Transposition of Insertion Sequences was Triggered by Oxidative Stress in Radiation-Resistant Bacterium Deinococcus geothermalis

2019 ◽  
Vol 7 (10) ◽  
pp. 446 ◽  
Author(s):  
Chanjae Lee ◽  
Nakjun Choi ◽  
Min K. Bae ◽  
Kyungsil Choo ◽  
Sung-Jae Lee
Keyword(s):  
Oxidative Stress ◽  
Family Member ◽  
Mutant Strain ◽  
Mutant Cell ◽  
Carotenoid Biosynthesis ◽  
Wild Type ◽  
Is Element ◽  
Deinococcus Geothermalis ◽  
Key Enzyme ◽  
Radiation Resistant

During an oxidative stress-response assay on a putative Dps-like gene-disrupted Δdgeo_0257 mutant strain of radiation-resistant bacterium Deinococcus geothermalis, a non-pigmented colony was observed among the normal reddish color colonies. This non-pigmented mutant cell subsequently displayed higher sensitivity to H2O2. While carotenoid has a role in protecting as scavenger of reactive oxygen species the reddish wild-type strain from radiation and oxidative stresses, it is hypothesized that the carotenoid biosynthesis pathway has been disrupted in the mutant D. geothermalis cell. Here, we show that, in the non-pigmented mutant cell of interest, phytoene desaturase (Dgeo_0524, crtI), a key enzyme in carotenoid biosynthesis, was interrupted by transposition of an ISDge7 family member insertion sequence (IS) element. RNA-Seq analysis between wild-type and Δdgeo_0257 mutant strains revealed that the expression level of ISDge5 family transposases, but not ISDge7 family members, were substantially up-regulated in the Δdgeo_0257 mutant strain. We revealed that the non-pigmented strain resulted from the genomic integration of ISDge7 family member IS elements, which were also highly up-regulated, particularly following oxidative stress. The transposition path for both transposases is a replicative mode. When exposed to oxidative stress in the absence of the putative DNA binding protein Dgeo_0257, a reddish D. geothermalis strain became non-pigmented. This transformation was facilitated by transposition of an ISDge7 family IS element into a gene encoding a key enzyme of carotenoid biosynthesis. Further, we present evidence of additional active transposition by the ISDge5 family IS elements, a gene that was up-regulated during the stationary phase regardless of the presence of oxidative stress.

Spirosoma taeanense sp. nov., a radiation resistant bacterium isolated from a coastal sand dune

2021 ◽  
Vol 114 (2) ◽  
pp. 151-159
Author(s):  
Ji Hee Lee ◽  
Jong-Hyun Jung ◽  
Min-Kyu Kim ◽  
Han Na Choe ◽  
Chi Nam Seong ◽  
...  
Keyword(s):  
Sand Dune ◽  
Resistant Bacterium ◽  
Coastal Sand Dune ◽  
Radiation Resistant ◽  
Coastal Sand

scholarly journals Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments

2003 ◽  
Vol 69 (8) ◽  
pp. 4575-4582 ◽  
Author(s):  
Hassan Brim ◽  
Amudhan Venkateswaran ◽  
Heather M. Kostandarithes ◽  
James K. Fredrickson ◽  
Michael J. Daly
Keyword(s):  
High Temperature ◽  
Radioactive Waste ◽  
Elevated Temperatures ◽  
Deinococcus Radiodurans ◽  
Nitrilotriacetic Acid ◽  
Thermophilic Bacterium ◽  
Prospective Development ◽  
Deinococcus Geothermalis ◽  
Radiation Resistant

ABSTRACT Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated a Hg(II)-resistant D. geothermalis strain capable of reducing Hg(II) at elevated temperatures and in the presence of 50 Gy/h. Additionally, D. geothermalis is capable of reducing Fe(III)-nitrilotriacetic acid, U(VI), and Cr(VI). These characteristics support the prospective development of this thermophilic radiophile for bioremediation of radioactive mixed waste environments with temperatures as high as 55°C.

Sign in / Sign up

Export Citation Format

Share Document