scholarly journals Precision enhancement of MALDI-TOF MS using high resolution peak detection and label-free alignment

PROTEOMICS ◽  
2008 ◽  
Vol 8 (8) ◽  
pp. 1530-1538 ◽  
Author(s):  
Maureen B. Tracy ◽  
Haijian Chen ◽  
Dennis M. Weaver ◽  
Dariya I. Malyarenko ◽  
Maciek Sasinowski ◽  
...  
Keyword(s):  
High Resolution ◽  
Peak Detection ◽  
Label Free ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals MALDI-TOF MS as a label-free approach to rapid inhibitor screening

2006 ◽  
Vol 17 (6) ◽  
pp. 815-822 ◽  
Author(s):  
Kenneth D. Greis ◽  
Songtao Zhou ◽  
Thomas M. Burt ◽  
Andrew N. Carr ◽  
Elizabeth Dolan ◽  
...  
Keyword(s):  
Label Free ◽  
Maldi Tof Ms ◽  
Inhibitor Screening ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid identification of bacterial isolates from wheat roots by high resolution whole cell MALDI-TOF MS analysis

2013 ◽  
Vol 165 (3-4) ◽  
pp. 167-174 ◽  
Author(s):  
Maria Isabel Stets ◽  
Artur Soares Pinto ◽  
Luciano Fernandes Huergo ◽  
Emanuel Maltempi de Souza ◽  
Vandeir Francisco Guimarães ◽  
...  
Keyword(s):  
High Resolution ◽  
Rapid Identification ◽  
Bacterial Isolates ◽  
Wheat Roots ◽  
Whole Cell ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Decoration of trastuzumab with short oligonucleotides: synthesis and detailed characterization

2017 ◽  
Vol 15 (42) ◽  
pp. 8923-8928 ◽  
Author(s):  
A. M. Carvalho ◽  
A. Manicardi ◽  
C. Véliz Montes ◽  
S. B. Gunnoo ◽  
R. J. Schneider ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
High Resolution ◽  
Metastatic Breast ◽  
Detailed Characterization ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Oligonucleotides Synthesis ◽  
Tof Ms

Trastuzumab, an antibody prescribed for the treatment of metastatic breast cancer, was covalently conjugated to oligonucleotides and characterized by high resolution MALDI-ToF MS.

High-resolution MALDI-TOF MS study on analysis of low-molecular-weight products from photo-oxidation of poly(3-hexylthiophene)

2015 ◽  
Vol 50 (8) ◽  
pp. 1006-1012 ◽  
Author(s):  
Junji Mizukado ◽  
Hiroaki Sato ◽  
Liang Chen ◽  
Yasumasa Suzuki ◽  
Shogo Yamane ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Resolution ◽  
Low Molecular Weight ◽  
Maldi Tof Ms ◽  
Photo Oxidation ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals MALDI-TOF mass spectrometry and high-resolution melting PCR for the identification of Mycoplasma bovis isolates

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Aric J. McDaniel ◽  
Rachel J. Derscheid
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Melting ◽  
Clinical Manifestations ◽  
Mycoplasma Bovis ◽  
Pcr Assay ◽  
Accurate Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Abstract Background Mycoplasma bovis is an important pathogen of cattle worldwide. Many different clinical manifestations of infection can occur, including respiratory disease, arthritis, and mastitis, causing heavy losses to beef and dairy industries. Because Mycoplasma species are slow-growing and fastidious, traditional identification methods are not cost- or time-effective, and improved methods are sought to streamline laboratory processes. High-resolution melting PCR (HRM-PCR) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) are 2 relatively recent tools that are rapid and inexpensive to use; we tested 9 isolates of M. bovis using both assays. The HRM-PCR assay used universal mycoplasma primers for the 16S–23S intergenic spacer region (IGSR). Results The resulting melting profiles of the field isolates were indistinguishable from the reference strain, indicating accurate identification. For the MALDI-TOF MS, each M. bovis isolate was accurately identified. Mycoplasma arginini and Mycoplasma alkalescens isolates did not identify as M. bovis when tested by either assay. Conclusions Our work shows that either assay could be used to identify unknown M. bovis isolates. For future work, the MALDI-TOF MS library should be expanded to include more mycoplasmas, and the HRM-PCR assay should be tested on additional mycoplasmas to ensure that the melting profiles are sufficiently distinctive.

Assessment of high-throughput high-resolution MALDI-TOF-MS of urinary peptides for the detection of muscle-invasive bladder cancer

2011 ◽  
Vol 5 (9-10) ◽  
pp. 493-503 ◽  
Author(s):  
Richard T. Bryan ◽  
Wenbin Wei ◽  
Neil J. Shimwell ◽  
Stuart I. Collins ◽  
Syed A. Hussain ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
High Resolution ◽  
High Throughput ◽  
Invasive Bladder Cancer ◽  
Muscle Invasive Bladder Cancer ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Muscle Invasive ◽  
Urinary Peptides ◽  
Tof Ms
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