A proteomic approach to identify differentially expressed plasma proteins between the fed and prolonged fasted states

PROTEOMICS ◽  
2009 ◽  
Vol 9 (1) ◽  
pp. 148-158 ◽  
Author(s):  
Fabrice Bertile ◽  
Christine Schaeffer ◽  
Yvon Le Maho ◽  
Thierry Raclot ◽  
Alain Van Dorsselaer
Keyword(s):  
Plasma Proteins ◽  
Differentially Expressed ◽  
Proteomic Approach

Identification of differentially expressed proteins in retinoblastoma tumors using mass spectrometry-based comparative proteomic approach

2017 ◽  
Vol 159 ◽  
pp. 77-91 ◽  
Author(s):  
Jasmine Naru ◽  
Ritu Aggarwal ◽  
Ashok Kumar Mohanty ◽  
Usha Singh ◽  
Deepak Bansal ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Proteomic Approach

scholarly journals 2-DE-MS based proteomic investigation of dairy cows with footrot

2016 ◽  
Vol 60 (1) ◽  
pp. 63-69
Author(s):  
Jiasan Zheng ◽  
Shi Shu ◽  
Cheng Xia ◽  
Chuang Xu ◽  
Hongyou Zhang ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Differentially Expressed ◽  
Control Group ◽  
Differentially Expressed Proteins ◽  
Defence Mechanisms ◽  
Flight Mass Spectrometry ◽  
Mass Spectrum Analysis ◽  
Proteomic Investigation ◽  
Dimensional Electrophoresis ◽  
Control Group Group

Abstract Introduction: The differentially expressed proteins between healthy cows and those with footrot were identified to explore changes in protein profiles associated with the disease. Material and Methods: Out of 36 cows selected for the experiment, 18 footrot-affected cows were included in the treatment group (group T) and 18 unaffected cows were included in the control group (group C). Plasma samples from groups T and C were subjected to two-dimensional electrophoresis analysis and differentially expressed proteins were identified by matrix-assisted laser desorption/ionisation tandem time-of-flight mass spectrometry. Bioinformatics, including gene ontology analysis and pathway analysis, was used for analysing all proteins. Results: Out of 63 spots identified by 2DE, 33 were selected for mass spectrum analysis, which identified 11 differentially expressed proteins in 26 spots. Footrot led to changes in profiles in plasma proteins that were classified to the pathway of inflammatory response, complement, and blood coagulation, among others. Conclusion: This study provides evidence of the defence mechanisms of cows with footrot to explore strategies for treatment.

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

2007 ◽  
Vol 30 (12) ◽  
pp. 1979-1988 ◽  
Author(s):  
Md Imtaiyaz Hassan ◽  
Vijay Kumar ◽  
Tara Kashav ◽  
Neelima Alam ◽  
Tej P. Singh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Tumor Proliferation ◽  
Proteomic Approach ◽  
Seminal Plasma Proteins

Proteomic analysis of the effects of lutein on mammary gland metabolism in dairy cows

2018 ◽  
Vol 85 (2) ◽  
pp. 152-156
Author(s):  
Caihong Wang ◽  
Chong Wang ◽  
Jianxin Liu ◽  
Hongyun Liu
Keyword(s):  
Blood Flow ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Control Diet ◽  
Differentially Expressed ◽  
Acid Oxidation ◽  
Differentially Expressed Proteins ◽  
Proteomic Approach ◽  
Increased Blood Flow

The aim of the research reported in this Research Communication was to identify differentially expressed proteins in dairy cows with normal and lutein diet and to elucidate the mechanisms of lutein-induced effects on bovine mammary gland metabolism using a comparative proteomic approach. Thirty-three differentially expressed proteins were identified from mammary gland of control diet-fed and lutein diet-fed dairy cows. Among these proteins, 15 were upregulated and 18 were downregulated in the lutein group. Functional analysis of the differentially expressed proteins showed that increased blood flow, depressed glycolysis, enhanced lactose anabolism, decreased fatty acid oxidation and up-regulated beta lactoglobulin expression were connected with lutein addition. These results suggested that the increased blood flow, reduced glucose catabolism, enhanced capacity for milk lactose synthesis, depressed fatty acid catabolism and increased expression of antioxidantion related protein may be the prime factors contributing to the increased milk production and enhanced immune status in lutein-fed dairy cows. This study provides molecular mechanism of dietary lutein in regulating lactation of dairy cows.

Proteomic approach to coronary atherosclerosis shows ferritin light chain as a significant marker: evidence consistent with iron hypothesis in atherosclerosis

2003 ◽  
Vol 13 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Sun-Ah You ◽  
Stephen R. Archacki ◽  
George Angheloiu ◽  
Christine S. Moravec ◽  
Shaoqi Rao ◽  
...  
Keyword(s):  
Coronary Arteries ◽  
Light Chain ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Spectrometric Analysis ◽  
Iron Storage ◽  
Mortality And Morbidity ◽  
Proteomic Approach ◽  
Normal Individuals ◽  
Artery Disease

Coronary artery disease (CAD) is the leading cause of mortality and morbidity in developed nations. We hypothesized that CAD is associated with distinct patterns of protein expression in the coronary arteries, and we have begun to employ proteomics to identify differentially expressed proteins in diseased coronary arteries. Two-dimensional (2-D) gel electrophoresis of proteins and subsequent mass spectrometric analysis identified the ferritin light chain as differentially expressed between 10 coronary arteries from patients with CAD and 7 coronary arteries from normal individuals. Western blot analysis indicated significantly increased expression of the ferritin light chain in the diseased coronary arteries (1.41 vs. 0.75; P = 0.01). Quantitative real-time PCR analysis showed that expression of ferritin light chain mRNA was decreased in diseased tissues (0.70 vs. 1.17; P = 0.013), suggesting that increased expression of ferritin light chain in CAD coronary arteries may be related to increased protein stability or upregulation of expression at the posttranscriptional level in the diseased tissues. Ferritin light chain protein mediates storage of iron in cells. We speculate that increased expression of the ferritin light chain may contribute to pathogenesis of CAD by modulating oxidation of lipids within the vessel wall through the generation of reactive oxygen species. Our results provide in situ proteomic evidence consistent with the “iron hypothesis,” which proposes an association between excessive iron storage and a high risk of CAD. However, it is also possible that the increased ferritin expression in diseased coronary arteries is a consequence, rather than a cause, of CAD.

scholarly journals Proteomics in Deaths by Drowning: Diagnostic Efficacy of Apolipoprotein A1 and α-1 Antitrypsin, Pilot Study

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 747
Author(s):  
Diana Hernández-Romero ◽  
Encarnación Sánchez-Rodríguez ◽  
Eduardo Osuna ◽  
Agustín Sibón ◽  
Miriam Martínez-Villanueva ◽  
...  
Keyword(s):  
Roc Curves ◽  
Apolipoprotein A1 ◽  
Differentially Expressed ◽  
Correct Classification ◽  
Postmortem Diagnosis ◽  
Diagnostic Efficacy ◽  
Proteomic Approach ◽  
Validation Experiment ◽  
Diagnosis Criteria

Drowning is one of the leading causes of death worldwide. The pathophysiology of drowning is complex and, sometimes, interpretation of the circumstances of death in the autopsy becomes the main source of information in its diagnosis. New advances in medical research, such as proteomics, especially in forensic pathology, are still in the development. We proposed to investigate the application of Mass Spectrometry-based technologies, to identify differentially expressed proteins that may act as potential biomarkers in the postmortem diagnosis of drowning. We performed a pilot proteomic experiment with the inclusion of two drowned and two control forensic cases. After applying restrictive parameters, we identified apolipoprotein A1 (ApoA1) and α-1 antitrypsin as differentially expressed between the two diagnostic groups. A validation experiment, with the determination of both proteins in 25 forensic cases (16 drowned and 9 controls) was performed, and we corroborated ApoA1 higher values in the drowning group, whereas α-1 antitrypsin showed lower levels. After adjusting by confounder factors, both remained as predictive independent factors for diagnosis of drowning (p = 0.010 and p = 0.022, respectively). We constructed ROC curves for biomarkers’ levels attending at the origin of death and established an ApoA1 cut-off point of 100 mg/dL. Correct classification based on the diagnosis criteria was reached for 73.9% of the cases in a discriminant analysis. We propose apolipoprotein A1 (with our cutoff value for correct classification) and α-1 antitrypsin as valuable biomarkers of drowning. Our study, based on forensic cases, reveals our proteomic approach as a new complementary tool in the forensic diagnosis of drowning and, perhaps, in clinical future implications in drowned patients. However, this is a pilot approach, and future studies are necessary to consolidate our promising preliminary data.

Identification of differentially expressed plasma proteins in atherosclerotic patients with type 2 diabetes

2016 ◽  
Vol 30 (5) ◽  
pp. 880-886 ◽  
Author(s):  
Antonio Junior Lepedda ◽  
Omar Lobina ◽  
Silvia Rocchiccioli ◽  
Gabriele Nieddu ◽  
Nadia Ucciferri ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Plasma Proteins ◽  
Differentially Expressed

scholarly journals Nitrogen Fertilizer Induced Alterations in The Root Proteome of Two Rice Cultivars

2019 ◽  
Vol 20 (15) ◽  
pp. 3674 ◽  
Author(s):  
Tang ◽  
Sun ◽  
Chen ◽  
Damaris ◽  
Lu ◽  
...  
Keyword(s):  
Crop Production ◽  
Oryza Sativa L ◽  
N Fertilizer ◽  
Differentially Expressed ◽  
Growth And Yield ◽  
Limiting Factor ◽  
Fertilizer Treatment ◽  
Rice Cultivars ◽  
Differentially Expressed Proteins ◽  
Proteomic Approach

Nitrogen (N) is an essential nutrient for plants and a key limiting factor of crop production. However, excessive application of N fertilizers and the low nitrogen use efficiency (NUE) have brought in severe damage to the environment. Therefore, improving NUE is urgent and critical for the reductions of N fertilizer pollution and production cost. In the present study, we investigated the effects of N nutrition on the growth and yield of the two rice (Oryza sativa L.) cultivars, conventional rice Huanghuazhan and indica hybrid rice Quanliangyou 681, which were grown at three levels of N fertilizer (including 135, 180 and 225 kg/hm2, labeled as N9, N12, N15, respectively). Then, a proteomic approach was employed in the roots of the two rice cultivars treated with N fertilizer at the level of N15. A total of 6728 proteins were identified, among which 6093 proteins were quantified, and 511 differentially expressed proteins were found in the two rice cultivars after N fertilizer treatment. These differentially expressed proteins were mainly involved in ammonium assimilation, amino acid metabolism, carbohydrate metabolism, lipid metabolism, signal transduction, energy production/regulation, material transport, and stress/defense response. Together, this study provides new insights into the regulatory mechanism of nitrogen fertilization in cereal crops.

scholarly journals Prioritizing Candidates of Post–Myocardial Infarction Heart Failure Using Plasma Proteomics and Single-Cell Transcriptomics

Circulation ◽  
2020 ◽  
Vol 142 (15) ◽  
pp. 1408-1421 ◽  
Author(s):  
Mark Y. Chan ◽  
Motakis Efthymios ◽  
Sock Hwee Tan ◽  
John W. Pickering ◽  
Richard Troughton ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Single Cell ◽  
Plasma Proteins ◽  
Meta Analysis ◽  
Left Ventricular ◽  
Differentially Expressed ◽  
Left Ventricular Ejection ◽  
Data Set ◽  
Ventricular Ejection Fraction ◽  
Plasma Proteomics

Background: Heart failure (HF) is the most common long-term complication of acute myocardial infarction (MI). Understanding plasma proteins associated with post-MI HF and their gene expression may identify new candidates for biomarker and drug target discovery. Methods: We used aptamer-based affinity-capture plasma proteomics to measure 1305 plasma proteins at 1 month post-MI in a New Zealand cohort (CDCS [Coronary Disease Cohort Study]) including 181 patients post-MI who were subsequently hospitalized for HF in comparison with 250 patients post-MI who remained event free over a median follow-up of 4.9 years. We then correlated plasma proteins with left ventricular ejection fraction measured at 4 months post-MI and identified proteins potentially coregulated in post-MI HF using weighted gene co-expression network analysis. A Singapore cohort (IMMACULATE [Improving Outcomes in Myocardial Infarction through Reversal of Cardiac Remodelling]) of 223 patients post-MI, of which 33 patients were hospitalized for HF (median follow-up, 2.0 years), was used for further candidate enrichment of plasma proteins by using Fisher meta-analysis, resampling-based statistical testing, and machine learning. We then cross-referenced differentially expressed proteins with their differentially expressed genes from single-cell transcriptomes of nonmyocyte cardiac cells isolated from a murine MI model, and single-cell and single-nucleus transcriptomes of cardiac myocytes from murine HF models and human patients with HF. Results: In the CDCS cohort, 212 differentially expressed plasma proteins were significantly associated with subsequent HF events. Of these, 96 correlated with left ventricular ejection fraction measured at 4 months post-MI. Weighted gene co-expression network analysis prioritized 63 of the 212 proteins that demonstrated significantly higher correlations among patients who developed post-MI HF in comparison with event-free controls (data set 1). Cross-cohort meta-analysis of the IMMACULATE cohort identified 36 plasma proteins associated with post-MI HF (data set 2), whereas single-cell transcriptomes identified 15 gene-protein candidates (data set 3). The majority of prioritized proteins were of matricellular origin. The 6 most highly enriched proteins that were common to all 3 data sets included well-established biomarkers of post-MI HF: N-terminal B-type natriuretic peptide and troponin T, and newly emergent biomarkers, angiopoietin-2, thrombospondin-2, latent transforming growth factor-β binding protein-4, and follistatin-related protein-3, as well. Conclusions: Large-scale human plasma proteomics, cross-referenced to unbiased cardiac transcriptomics at single-cell resolution, prioritized protein candidates associated with post-MI HF for further mechanistic and clinical validation.

scholarly journals Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails

Hematology ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 300-307 ◽  
Author(s):  
Jirawan Panachan ◽  
Daranee Chokchaichamnankit ◽  
Churat Weeraphan ◽  
Chantragan Srisomsap ◽  
Patarabutr Masaratana ◽  
...  
Keyword(s):  
Vitamin E ◽  
Plasma Proteins ◽  
Differentially Expressed ◽  
Hemoglobin E
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