scholarly journals Proteomic analysis of day–night variations in protein levels in the rat pineal gland

PROTEOMICS ◽  
2007 ◽  
Vol 7 (12) ◽  
pp. 2009-2018 ◽  
Author(s):  
Morten Møller ◽  
Thomas Sparre ◽  
Nicolai Bache ◽  
Peter Roepstorff ◽  
Henrik Vorum
Keyword(s):  
Pineal Gland ◽  
Proteomic Analysis ◽  
Protein Levels ◽  
Rat Pineal Gland

scholarly journals Melatonin: an endogenous negative modulator of 12-lipoxygenation in the rat pineal gland

1999 ◽  
Vol 344 (2) ◽  
pp. 487-493 ◽  
Author(s):  
Hongjian ZHANG ◽  
Mohammed AKBAR ◽  
Hee-Yong KIM
Keyword(s):  
Pineal Gland ◽  
Negative Ion ◽  
Dark Phase ◽  
Exogenous Melatonin ◽  
Protein Levels ◽  
Endogenous Modulator ◽  
12 Lipoxygenase ◽  
Rat Pineal Gland ◽  
Melatonin Production

Major biochemical activities of the pineal gland include melatonin biosynthesis and 12-lipoxygenation. In this paper, we provide evidence in vivo that melatonin regulates 12-lipoxygenation via 12-lipoxygenase (LOX) expression. The relationship between these two biochemical activities was established by monitoring levels of endogenous melatonin and a 12-LOX metabolite, 12-hydroxyeicosatetraenoic acid (12-HETE), in the rat pineal gland both during the light-dark cycle and after isoproterenol injection using GC/MS with negative ion chemical ionization. As pineal melatonin production reflected a typical diurnal variation, 12-HETE levels showed an off-phase diurnal pattern in relation to melatonin levels. Intravenous administration of isoproterenol, which has been shown to elevate melatonin production, decreased the 12-HETE level significantly. The reduction of 12-HETE levels during the dark phase and after isoproterenol injection was accompanied by decreases in 12-LOX mRNA and protein levels. Direct administration of melatonin to rats by intravenous injection decreased pineal 12-LOX protein levels significantly, indicating that melatonin plays a role in down-regulating 12-LOX expression. When pineal glands were incubated with exogenous melatonin in culture, time-dependent reduction of 12-LOX protein levels was observed. The melatonin-induced reduction in 12-LOX protein was abolished in the presence of the melatonin receptor antagonist luzindole, establishing further the role of melatonin in this process. Incubation of pineal homogenates with exogenous melatonin partially inhibited 12-LOX activity. Taken together, an inverse relationship exists in the endogenous production of 12-HETE, 12-LOX mRNA and protein with respect to melatonin production in the rat pineal gland. Melatonin decreased both 12-LOX mRNA and protein levels in addition to 12-LOX enzyme activity, indicating that melatonin is an endogenous modulator of pineal 12-lipoxygenation.

Circadian Variation of Cyclic AMP in the Rat Pineal Gland

1981 ◽  
Vol 36 (3) ◽  
pp. 1295-1297 ◽  
Author(s):  
Masahiko Mikuni ◽  
Yoshiro Saito ◽  
Tsukasa Koyama ◽  
Itaru Yamashita
Keyword(s):  
Cyclic Amp ◽  
Pineal Gland ◽  
Circadian Variation ◽  
Rat Pineal Gland

Daily rhythm and regulation of clock gene expression in the rat pineal gland

2004 ◽  
Vol 120 (2) ◽  
pp. 164-172 ◽  
Author(s):  
V Simonneaux ◽  
V.-J Poirel ◽  
M.-L Garidou ◽  
D Nguyen ◽  
E Diaz-Rodriguez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pineal Gland ◽  
Clock Gene ◽  
Daily Rhythm ◽  
Clock Gene Expression ◽  
Rat Pineal Gland

Presynaptic effects of melatonin on norepinephrine release and uptake in rat pineal gland

1991 ◽  
Vol 10 (4) ◽  
pp. 165-173 ◽  
Author(s):  
Héctor E. Chuluyan ◽  
Ruth E. Rosenstein ◽  
Su Min Chang ◽  
María M. Gálvez ◽  
Daniel P. Cardinali
Keyword(s):  
Pineal Gland ◽  
Norepinephrine Release ◽  
Rat Pineal Gland

Estrogen modulation of adrenoceptor responsiveness in the female rat pineal gland: differential expression of intracellular estrogen receptors

2004 ◽  
Vol 37 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Juan J. Sanchez ◽  
Pedro Abreu ◽  
Tomas Gonzalez-Hernandez ◽  
Alexis Hernandez ◽  
Luis Prieto ◽  
...  
Keyword(s):  
Pineal Gland ◽  
Estrogen Receptors ◽  
Differential Expression ◽  
Female Rat ◽  
Rat Pineal Gland

scholarly journals Pretranslational Regulation of Rhythmic Type II Iodothyronine Deiodinase Expression by β-Adrenergic Mechanism in the Rat Pineal Gland*

Endocrinology ◽  
1999 ◽  
Vol 140 (3) ◽  
pp. 1272-1278 ◽  
Author(s):  
Yuji Kamiya ◽  
Masami Murakami ◽  
Osamu Araki ◽  
Yasuhiro Hosoi ◽  
Takayuki Ogiwara ◽  
...  
Keyword(s):  
Pineal Gland ◽  
Type Ii ◽  
Iodothyronine Deiodinase ◽  
Adrenergic Mechanism ◽  
Rat Pineal Gland

Rat Pineal Gland Suppresses the Injection Stress-Reactive ACTH Outflow

1993 ◽  
Vol 25 (03) ◽  
pp. 149-151 ◽  
Author(s):  
J. Milin ◽  
K. Djakovic-Svajcer ◽  
M. Demajo
Keyword(s):  
Pineal Gland ◽  
Rat Pineal Gland
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