Proteomic analysis of human very low-density lipoprotein by two-dimensional gel electrophoresis and MALDI-TOF/TOF

PROTEOMICS ◽  
2007 ◽  
Vol 7 (1) ◽  
pp. 143-154 ◽  
Author(s):  
Carmine Mancone ◽  
Laura Amicone ◽  
Gian Maria Fimia ◽  
Elena Bravo ◽  
Mauro Piacentini ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Maldi Tof

scholarly journals Identification and distribution of proteins in isolated endosomal fractions of rat liver: involvement in endocytosis, recycling and transcytosis

1997 ◽  
Vol 323 (2) ◽  
pp. 435-443 ◽  
Author(s):  
Albert POL ◽  
David ORTEGA ◽  
Carlos ENRICH
Keyword(s):  
Gel Electrophoresis ◽  
Low Density Lipoprotein ◽  
Growth Factor Receptor ◽  
Density Lipoprotein ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Immunoglobulin Receptor ◽  
Early Endosomes

1. The polypeptides of the three endosomal fractions isolated from livers of oestradiol-treated rats were analysed by two-dimensional gel electrophoresis. Silver-stained gels revealed that although the three endosomal fractions shared a generally similar pattern of approx. 120 components, qualitative and quantitative differences between the three endocytic fractions could be demonstrated. 2. The ‘early’ endosomes [compartment of uncoupling of receptors and ligands (CURL)] comprised the most complex fraction and contained most of the polypeptides found in the ‘late’ endosomes [multivesicular bodies (MVBs)] and the receptor recycling compartment (RRC). When CURL was analysed by two-dimensional gel electrophoresis after partition with Triton X-114, it showed the largest number of integral membrane polypeptides. 3. Some of the major receptors (polymeric immunoglobulin receptor, transferrin receptor, low-density lipoprotein receptor, asialoglycoprotein receptor, β1-integrin, mannose 6-phosphate receptor, epidermal growth factor receptor and AGp110) and internalized ligands (IgA, IgG, albumin, haptoglobin, transferrin and α2-macroglobulin) were further studied by Western blotting. 4. The distribution of the identified receptors and ligands among the three endosomal fractions was in agreement with their expected functionalities. 5. The polypeptide composition of the bile was also examined and compared with ligands and proteins identified in the different endocytic fractions. 6. Finally, an electron microscopy study confirms the distinctive physical and ultrastructural features of the three isolated endosomal fractions.

scholarly journals Proteomic Analysis of the Very Low Density Lipoprotein (VLDL) transport vesicles

2012 ◽  
Vol 75 (7) ◽  
pp. 2225-2235 ◽  
Author(s):  
Abdul Rahim ◽  
Erika Nafi-valencia ◽  
Shaila Siddiqi ◽  
Riyaz Basha ◽  
Chukwuemeka C. Runyon ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Transport Vesicles

Studies on the Thromboplastic Effect of Human Plasma Lipoproteins

1976 ◽  
Vol 35 (01) ◽  
pp. 178-185 ◽  
Author(s):  
Helena Sandberg ◽  
Lars-Olov Andersson
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Platelet Rich Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Free Plasma ◽  
Good Agreement

SummaryHuman plasma lipoprotein fractions were prepared by flotation in the ultracentrifuge. Addition of these fractions to platelet-rich, platelet-poor and platelet-free plasma affected the partial thromboplastin and Stypven clotting times to various degrees. Addition of high density lipoprotein (HDL) to platelet-poor and platelet-free plasma shortened both the partial thromboplastin and the Stypven time, whereas addition of low density lipoprotein and very low density lipoprotein (LDL + VLDL) fractions only shortened the Stypven time. The additions had little or no effect in platelet-rich plasma.Experiments involving the addition of anti-HDL antibodies to plasmas with different platelet contents and measuring of clotting times produced results that were in good agreement with those noted when lipoprotein was added. The relation between structure and the clot-promoting activity of various phospholipid components is discussed.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

Sign in / Sign up

Export Citation Format

Share Document