Gel-free analysis of the human brain proteome: Application of liquid chromatography and mass spectrometry on biopsy and autopsy samples

PROTEOMICS ◽  
2006 ◽  
Vol 6 (18) ◽  
pp. 4967-4977 ◽  
Author(s):  
Debora Dumont ◽  
Jean-Paul Noben ◽  
Peter Verhaert ◽  
Piet Stinissen ◽  
Johan Robben
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Human Brain ◽  
Brain Proteome

scholarly journals Recent Developments in Data Independent Acquisition (DIA) Mass Spectrometry: Application of Quantitative Analysis of the Brain Proteome

2020 ◽  
Vol 13 ◽  
Author(s):  
Ka Wan Li ◽  
Miguel A. Gonzalez-Lozano ◽  
Frank Koopmans ◽  
August B. Smit
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Brain Research ◽  
Proteome Analysis ◽  
Protein Isolate ◽  
Elution Time ◽  
Data Independent Acquisition ◽  
Peptide Precursor ◽  
Brain Proteome

Mass spectrometry is the driving force behind current brain proteome analysis. In a typical proteomics approach, a protein isolate is digested into tryptic peptides and then analyzed by liquid chromatography–mass spectrometry. The recent advancements in data independent acquisition (DIA) mass spectrometry provide higher sensitivity and protein coverage than the classic data dependent acquisition. DIA cycles through a pre-defined set of peptide precursor isolation windows stepping through 400–1,200 m/z across the whole liquid chromatography gradient. All peptides within an isolation window are fragmented simultaneously and detected by tandem mass spectrometry. Peptides are identified by matching the ion peaks in a mass spectrum to a spectral library that contains information of the peptide fragment ions' pattern and its chromatography elution time. Currently, there are several reports on DIA in brain research, in particular the quantitative analysis of cellular and synaptic proteomes to reveal the spatial and/or temporal changes of proteins that underlie neuronal plasticity and disease mechanisms. Protocols in DIA are continuously improving in both acquisition and data analysis. The depth of analysis is currently approaching proteome-wide coverage, while maintaining high reproducibility in a stable and standardisable MS environment. DIA can be positioned as the method of choice for routine proteome analysis in basic brain research and clinical applications.

888: Metabolomic Urine Samples Profiling Using High Performance Liquid Chromatography Coupled with Mass Spectrometry to Detect Bladder Cancer

2007 ◽  
Vol 177 (4S) ◽  
pp. 295-295
Author(s):  
Michael Mullerad ◽  
Haleem J. Issaq ◽  
Alexander Kravtsov ◽  
Timothy Waybright ◽  
Brian Luke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Bladder Cancer ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Urine Samples
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