Plasma proteomics of lung cancer by a linkage of multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis

PROTEOMICS ◽  
2006 ◽  
Vol 6 (13) ◽  
pp. 3938-3948 ◽  
Author(s):  
Tetsuya Okano ◽  
Tadashi Kondo ◽  
Tatsuhiko Kakisaka ◽  
Kiyonaga Fujii ◽  
Masayo Yamada ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Liquid Chromatography ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis ◽  
Plasma Proteomics

scholarly journals Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Tatsuo Hagiwara ◽  
Yumi Saito ◽  
Yukiko Nakamura ◽  
Takeshi Tomonaga ◽  
Yasufumi Murakami ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Large Scale ◽  
Protein Identification ◽  
Solid Phase ◽  
Mass Spectrometric ◽  
Two Dimensional ◽  
Anion Exchange Column ◽  
Difference Gel Electrophoresis ◽  
Plasma Proteomics

The intact plasma proteome is of great interest in biomarker studies because intact proteins reflect posttranslational protein processing such as phosphorylation that may correspond to disease status. We examined the utility of a solid-phase hexapeptide ligand library in combination with conventional plasma proteomics modalities for comprehensive profiling of intact plasma proteins. Plasma proteins were sequentially fractionated using depletion columns for albumin and immunoglobulin, and separated using an anion-exchange column. Proteins in each fraction were treated with a solid-phase hexapeptide ligand library and compared to those without treatment. Two-dimensional difference gel electrophoresis demonstrated an increased number of protein spots in the treated samples. Mass spectrometric studies of these protein spots with unique intensity in the treated samples resulted in the identification of high- and medium-abundance proteins. Our results demonstrated the possible utility of a solid-phase hexapeptide ligand library to reveal greater number of intact plasma proteins. The characteristics of proteins with unique affinity to the library remain to be clarified by more extensive mass spectrometric protein identification, and optimized protocols should be established for large-scale plasma biomarker studies.

Two-Dimensional Difference Gel Electrophoresis

2018 ◽  
pp. 229-247 ◽  
Author(s):  
Malachi Blundon ◽  
Vinitha Ganesan ◽  
Brendan Redler ◽  
Phu T. Van ◽  
Jonathan S. Minden
Keyword(s):  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis

scholarly journals Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis

2007 ◽  
Vol 6 (10) ◽  
pp. 1798-1808 ◽  
Author(s):  
Wei Sun ◽  
Baocai Xing ◽  
Yi Sun ◽  
Xiaojuan Du ◽  
Min Lu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Difference Gel Electrophoresis
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